A Bima Array Survey of Molecules in Comets Linear (C/2002 T7) and Neat (C/2001 Q4)

We present an interferometric search for large molecules, including methanol, methyl cyanide, ethyl cyanide, ethanol, and methyl formate in comets LINEAR (C/2002 T7) and NEAT (C/2001 Q4) with the Berkeley-Illinois-Maryland Association (BIMA) array. In addition, we also searched for transitions of the simpler molecules CS, SiO, HNC, HN13C and 13CO . We detected transitions of methanol and CS around Comet LINEAR and one transition of methanol around Comet NEAT within a synthesized beam of ~20''. We calculated the total column density and production rate of each molecular species using the variable temperature and outflow velocity (VTOV) model described by Friedel et al.(2005).Considering the molecular production rate ratios with respect to water, Comet T7 LINEAR is more similar to Comet Hale-Bopp while Comet Q4 NEAT is more similar to Comet Hyakutake. It is unclear, however, due to such a small sample size, whether there is a clear distinction between a Hale-Bopp and Hyakutake class of comet or whether comets have a continuous range of molecular production rate ratios.Comment: Accepted for Publication in the Astrophysical Journa

BIMA Array Detections of HCN in Comets LINEAR (C/2002 T7) and NEAT (C/2001 Q4)

We present interferometric detections of HCN in comets LINEAR (C/2002 T7) and NEAT (C/2001 Q4) with the Berkeley-Illinois-Maryland Association (BIMA) Array. With a 25.4"x20.3" synthesized beam around Comet LINEAR and using a variable temperature and outflow velocity (VTOV) model, we found an HCN column density of N_T=6.4+-2.1x10^12 cm^-2, and a production rate of Q(HCN)=6.5+-2.2x10^26 s^-1, giving a production rate ratio of HCN relative to H_2O of ~3.3+-1.1x10^-3 and relative to CN of ~4.6+-1.5. With a 21.3"x17.5" synthesized beam around Comet NEAT and using a VTOV model, we found an HCN column density of N_T=8.5+-4.5x10^11 cm^-2, and a production rate of Q(HCN)=8.9+-4.7x10^25 s^-1, giving a production rate ratio of HCN relative to H_2O of ~7.4+-3.9x10^-4 and relative to CN of ~0.3+-0.2. For both comets, the production rates relative to H_2O are similar to those found in previous comet observations. For Comet LINEAR the production rate relative to CN is consistent with HCN being the primary parent species of CN, while for Comet NEAT it is too low for this to be the case.Comment: 20 pages, 2 figures, to be published in the Astrophysical Journa

Evaluating the relationship between amyloid-β and α-synuclein phosphorylated at Ser129 in dementia with Lewy bodies and Parkinson’s disease

INTRODUCTION: Lewy body and Alzheimer-type pathologies often co-exist. Several studies suggest a synergistic relationship between amyloid-β (Aβ) and α-synuclein (α-syn) accumulation. We have explored the relationship between Aβ accumulation and the phosphorylation of α-syn at serine-129 (pSer129 α-syn), in post-mortem human brain tissue and in SH-SY5Y neuroblastoma cells transfected to overexpress human α-syn. METHODS: We measured levels of Aβ40, Aβ42, α-syn and pSer129 α-syn by sandwich enzyme-linked immunosorbent assay, in soluble and insoluble fractions of midfrontal, cingulate and parahippocampal cortex and thalamus, from cases of Parkinson’s disease (PD) with (PDD; n = 12) and without dementia (PDND; n = 23), dementia with Lewy bodies (DLB; n = 10) and age-matched controls (n = 17). We also examined the relationship of these measurements to cognitive decline, as measured by time-to-dementia and the mini-mental state examination (MMSE) score in the PD patients, and to Braak tangle stage. RESULTS: In most brain regions, the concentration of insoluble pSer129 α-syn correlated positively, and soluble pSer129 α-syn negatively, with the levels of soluble and insoluble Aβ. Insoluble pSer129 α-syn also correlated positively with Braak stage. In most regions, the levels of insoluble and soluble Aβ and the proportion of insoluble α-syn that was phosphorylated at Ser129 were significantly higher in the PD and DLB groups than the controls, and higher in the PDD and DLB groups than the PDND brains. In PD, the MMSE score correlated negatively with the level of insoluble pSer129 α-syn. Exposure of SH-SY5Y cells to aggregated Aβ42 significantly increased the proportion of α-syn that was phosphorylated at Ser129 (aggregated Aβ40 exposure had a smaller, non-significant effect). CONCLUSIONS: Together, these data show that the concentration of pSer129 α-syn in brain tissue homogenates is directly related to the level of Aβ and Braak tangle stage, and predicts cognitive status in Lewy body diseases. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13195-014-0077-y) contains supplementary material, which is available to authorized users