INSTITUTIONAL SECURITY IN RELATIONS OF OWNERSHIP OF NATURAL RESOURCES: STATE ENVIRONMENTAL AND ECONOMIC POLICY AND DECENTRALIZATION

The article defines the natural resource sphere of the national economy as the largest territorial and sectoral complex, which includes land, water, forest, mineral, natural and recreational and other types of resources. The lack of implementation of the allocation of a sufficient amount of funds aimed at the rationalization of nature use and the modernization of environmental infrastructure facilities, as well as the uneven distribution of capital investments for the protection and rational use of natural resources in the region for the period 2016-2022, was established. It is substantiated that in the conditions of decentralization of power, the general basis of institutional support for property relations over natural resources should be corporatization, development of rent relations, improvement of fiscal mechanisms in resource use, market-economic evaluation and capitalization of all-natural resources without exception at the local, regional and national levels. The theoretical foundations of the problems of institutional support in property relations in the conditions of decentralization of power have been deepened by determining the need to create an institutional system of property. This system provides for the development of lease relationships, approaches to investment projects, improvement of natural resource taxation mechanisms by improving the system of local taxes on the use of resources, and assessment of the value of natural resources at the micro, meso, and macro levels. The definition of ecological and economic problems of property relations is formulated, which, unlike the existing ones, are focused on the problems of attracting capital investments and current costs of the state for the protection of the natural environment in conditions of decentralization, as well as the fundamental basis for the formation of an institutional system of property in nature management, taking into account foreign experience. The results of the research can be used in the development of regional and national programs and forecasts on issues of nature management and environmental protection

Entrepreneurial initiative as a factor for the development of the innovation activity of country enterprises

The purpose of the study is to determine the impact of entrepreneurial initiative on the development of innovation activity of country enterprises. The theory of entrepreneurship by clarifying the essence of entrepreneurial initiative got further elaboration in the article. The methodical approach to the analysis and evaluation of the entrepreneurial initiative and its impact on the innovation activity of the enterprises, the results of which are the basis for further development of proposals to stimulate entrepreneurial initiative and development of their innovation activity is suggested. The estimation of innovation activity of 1758 enterprises of Ukraine by calculating the integral indicator of innovation activity revealed its low level for innovation active enterprises. The method of one-dimensional linear regression has determined that an increase in the number of business structures per 10 thousand people of the country's population at a rate of 1.0 causes an increase in the indicator of innovation activity by 0.172. It is proved in the article that development of infrastructure of enterprenerial environment and creation of centers for entrepreneurship development act as an important mechanism of increasing innovation activity of enterprises of the country

How Small Organic Osmolytes Modulate Actin Filament Assembly Kinetics and Mechanics

Actin filament assembly and mechanics are crucial for muscle contraction, maintenance of cell structure, motility, and division. Actin filament assembly occurs in a crowded intracellular environment consisting of various types of molecules, including small organic molecules known as osmolytes. Ample evidence highlights the protective functions of osmolytes such as trimethylamine-N-oxide (TMAO), including their effects on protein stability and their ability to counteract cellular osmotic stress. Recently, TMAO has been shown to counteract the denaturing effects of urea on actin filament assembly based on bulk fluorescence assays. Yet, how TMAO affects individual actin filament assembly dynamics and mechanics is not well understood. We hypothesize that, owing to its protective nature, TMAO will enhance filament assembly kinetics and stiffen actin filaments due to increased stability. In this study, we investigate osmolyte-dependent actin filament assembly kinetics and bending mechanics by measuring filament elongation rates and bending persistence lengths in the presence of TMAO using total internal reflection fluorescence (TIRF) microscopy imaging and pyrene fluorescence assays. We demonstrate that TMAO enhances increases the elongation rates of individual filaments as well as steady-state average filament lengths and enhances filament bending stiffness. Taken together, our results show that the physiochemical properties of the intracellular environment can regulate actin assembly dynamics and mechanics. This study will help identify molecular mechanisms of how small organic osmolytes modulate cytoskeletal protein filament assembly and mechanics in living cells

Atrial Reentry Tachycardia: Mechanisms and Diagnosis

Atrial tachycardia (AT) is a group of arrhythmias that differ in electrophysiological mechanisms and clinical course. There are three main forms of AT: focal AT, macroreentries, and localized reentries also known as “microreentries”. Macroreentries, as a rule, occur in the presence of fibrous changes in the myocardium, for example, in “atrial” cardiomyopathy or as a result of catheter or surgical interventions in the atria. Focal AT can also occur in the absence of structural pathology of the heart. The aim. To analyze modern ideas about electrophysiological mechanisms and diagnosis of atrial reentry tachycardia. In our research we used data from the literature as well as findings of our own previous studies. The work analyzed global data on the distribution of various types of AT. The data of electrocardiographic (ECG) diagnosis, electrophysiological methods of diagnosis and pharmacological tests were also analyzed to determine the mechanism of occurrence and course of AT. The main ECG differences of different types and localizations of AT were determined. The diagnostic value of various stimulation protocols in the differential diagnosis of focal and macro-reentrant AT was also analyzed. Conclusions. Atrial tachycardias with a reentrant mechanism are common in the general population. Differential diagnosis with other types of tachycardia is carried out on the basis of ECG data, the response to adenosine administration, the nature of the response during entrainment, and endocardial, particularly electroanatomical, mapping

Features of Catheter Treatment of Non-Isthmus-Dependent Atrial Flutter

Atrial flutter (AF) is caused by the re-circulation of the wave of electrical excitation of the myocardium (reentry) around the anatomical substrate which circulates within the atrial tissues. This is always a macro re-entry. Such an anatomical barrier, around which circulation can occur, can be the superior or inferior vena cava, rings of the tricuspid or mitral valves, the mouth of the coronary sinus, pulmonary veins, postoperative scar. The aim. To determine the specifics of elimination, success rate, and long-term outcome with various preablation and postablation diagnostic techniques for non-isthmus-dependent atrial flutter (NIDAFL). Materials and methods. The study included 26 patients who underwent radiofrequency ablation of atypical NIDAFL. Results. As a result of radiofrequency ablation, a sinus rhythm was restored in 17 patients during the procedure. In 7 cases, when the typical, isthmus-dependent AF was removed, the tachycardia cycle and the morphology of the R wave changed. Mapping showed that in 7 cases the direction of the re-entry front changed, and instead of the circulation of excitation through the cavatricuspid isthmus, it then passed around the atriotomy scar. In 2 cases, a change in the cardiac cycle was observed after radiofrequency ablation, but the excitation circulation was the same around the atriotomy scar, only the tachycardia cycle increased. As a result of the use of our techniques, arrhythmia was eliminated in all 21 patients with an atriotomy AF during one procedure. Five patients with AF of a different localization of the re-entry circuit also had their arrhythmia eliminated, although 8 procedures (for five patients) were performed (on average 1.6). There were no complications. During the follow-up period of 1.8±0.7 years, 2 patients had a recurrence of arrhythmia, and they underwent a repeat procedure to eliminate the arrhythmia. One patient developed typical AF that had not been observed before, which was successfully eliminated. Conclusion. Catheter treatment of atypical NIDAFL is quite a non-trivial task, because, as our experience shows, several types of tachycardia occur in a significant number of patients. In cases of restoration of sinus rhythm as a result of the application, it is necessary to check the inducibility of another arrhythmia. But despite everything, catheter removal of NIDAFL is quite effective, especially for atriotomy AF, although it requires more X-ray exposure and a relatively large number of applications. The use of navigation systems has helped to improve the results of such interventions in more complex cases