22 research outputs found
Caracterização citogenética para identificação dos níveis de ploidia em cinco espécies do gênero Mentha L.
Full text linkSomatic hybridization in mint: identification and characterization of Mentha piperita (+) M. spicata hybrid plants
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Transformation of the limonene synthase gene into peppermint (Mentha piperita L.) and preliminary studies on the essential oil profiles of single transgenic plants
No full textDevelopment of regeneration system and study of the transformation potential of a commercial tomato variety
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Towards reducing genotype specificity in regeneration protocols after somatic hybridization between cultivated sunflower and wild helianthus species
No full textSuccessful application of somatic hybridization in sunflower breeding is limited by low regeneration percentage from fusion products. The effect of pre-treatment media composition on shoot regeneration from electrofusion products of wild and cultivated sunflower was investigated in this paper, with the aim to determine if there is room for improvement of the existing regeneration protocols in order to make them less genotype-dependent. Protoplasts isolated from hypocotyls of two inbred lines of cultivated sunflower were electrofused with mesophyll protoplasts of H. mollis and H. maximiliani. The regeneration was induced only on calluses that were grown on the media with low auxin and high cytokinin contents during the development of calluses from microcalluses (pre-treatment), with silver nitrate as a medium component. Regeneration from fusion products was induced by treatment with a high concentration of TDZ or 2,4 D for a short period of time
Two critical factors are required for efficient transformation of multiple soybean cultivars: Agrobacterium strain and orientation of immature cotyledonary explant
No full textEffect of an enhanced CaMV 35S promoter and a fruit-specific promoter on uida gene expression in transgenic tomato plants
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