The Effect of Diel Temperature and Light Cycles on the Growth of Nannochloropsis oculata in a Photobioreactor Matrix

A matrix of photobioreactors integrated with metabolic sensors was used to examine the combined impact of light and temperature variations on the growth and physiology of the biofuel candidate microalgal species Nannochloropsis oculata. The experiments were performed with algal cultures maintained at a constant 20u C versus a 15°C to 25°C diel temperature cycle, where light intensity also followed a diel cycle with a maximum irradiance of 1920 μmol photons m-2 s-1. No differences in algal growth (Chlorophyll a) were found between the two environmental regimes; however, the metabolic processes responded differently throughout the day to the change in environmental conditions. The variable temperature treatment resulted in greater damage to photosystem II due to the combined effect of strong light and high temperature. Cellular functions responded differently to conditions before midday as opposed to the afternoon, leading to strong hysteresis in dissolved oxygen concentration, quantum yield of photosystem II and net photosynthesis. Overnight metabolism performed differently, probably as a result of the temperature impact on respiration. Our photobioreactor matrix has produced novel insights into the physiological response of Nannochloropsis oculata to simulated environmental conditions. This information can be used to predict the effectiveness of deploying Nannochloropsis oculata in similar field conditions for commercial biofuel production. © 2014 Tamburic et al

Characterization of Lunar Swirls at Mare Ingenii: A Model for Space Weathering at Magnetic Anomalies

Analysis of spectra from the Clementine ultraviolet-visible and near-infrared cameras of small, immature craters and surface soils both on and adjacent to the lunar swirls at Marc Ingenii has yielded the following conclusions about space weathering at a magnetic anomaly. (l) Despite having spectral characteristics of immaturity, the lunar swirls arc not freshly exposed surfaces. (2) The swirl surfaces arc regions of retarded weathering, while immediately adjacent regions experience accelerated weathering, (3) Weathering in the off-swirl regions darkens and flattens the spectrum with little to no reddening, which suggests that the production of larger (greater than 40 nm) nanophase iron dominates in these locations as a result of charged particle sorting by the magnetic field. Preliminaty analysis of two other lunar swirl regions, Reiner Gamma and Mare Marginis, is consistent with our observations at Mare Ingenii. Our results indicate that sputtering/vapor deposition, implanted solar wind hydrogen, and agglutination share responsibility for creating the range in npFe(sup 0) particle sizes responsible for the spectral effects of space weathering