Synthesis and in vitro antitumoral activity of novel O,O '-di-2-alkyl-(S,S)-ethylenediamine-N,N '-di-2-propanoate ligands and corresponding platinum(II/IV) complexes

Syntheses of two novel ligand precursors O,O'-diisopropyl- (1a) and O,O'-diisobutyl-(S,S)-ethylenediamine-N,N'-di-2-propanoate dihydrochloride monohydrate (1b) and the corresponding dichloroplatinum(II) (2a and 2b) and tetrachloroplatinum(IV) complexes (3a and 3b) are described here. The substances were characterized by IR, H-1 and C-13 spectroscopy and elemental analysis. Crystal structures were determined for la and the corresponding platinum(IV) complex, 3a. In vitro antiproliferative activity was determined against tumor cell lines: human adenocarcinoma HeLa, human myelogenous leukemia K562, human malignant melanoma Fem-x, rested and stimulated normal immunocompetent cells (human peripheral blood mononuclear PBMC cells) using KBR test (Kenacid Blue Dye binding test). The IC50(mu M) values for the most active compound 3a were: 30.48 +/- 2.54; 12.26 +/- 2.60; 13.68 +/- 3.22; 80.18 +/- 24.07 and 71.30 +/- 21.70, respectively. (C) 2008 Elsevier Inc. All rights reserved

Subgingival periodontopathogens and host-response factors in GCF as co-biomarkers for assessment of periodontal diseases

To emphasize the anisotropy induced by damage on a ceramic matrix composite subjected to a tensile solicitation at 30° from one of the fiber directions, the changes of all the stiffness tensor components are determined from wave speed measurements of obliquely incident ultrasonic bulk waves. Using the additive form of the stiffness tensor of the damaged material, the crack orientation is determined from the damage tensor, i.e., the part relative to the loss of stiffness caused by the microcracks. The search for the principal frame of this tensor from wave speed measurements allows the assessment of the microcrack orientation