5 research outputs found
Folding of the Triangular Lattice with Quenched Random Bending Rigidity
We study the problem of folding of the regular triangular lattice in the
presence of a quenched random bending rigidity + or - K and a magnetic field h
(conjugate to the local normal vectors to the triangles). The randomness in the
bending energy can be understood as arising from a prior marking of the lattice
with quenched creases on which folds are favored. We consider three types of
quenched randomness: (1) a ``physical'' randomness where the creases arise from
some prior random folding; (2) a Mattis-like randomness where creases are
domain walls of some quenched spin system; (3) an Edwards-Anderson-like
randomness where the bending energy is + or - K at random independently on each
bond. The corresponding (K,h) phase diagrams are determined in the hexagon
approximation of the cluster variation method. Depending on the type of
randomness, the system shows essentially different behaviors.Comment: uses harvmac (l), epsf, 17 figs included, uuencoded, tar compresse
Understanding cell fate acquisition in stem-cell-derived pancreatic islets using single-cell multiome-inferred regulomes
Pancreatic islet cells derived from human pluripotent stem cells hold great promise for modeling and treating diabetes. Differences between stem-cell-derived and primary islets remain, but molecular insights to inform improvements are limited. Here, we acquire single-cell transcriptomes and accessible chromatin profiles during in vitro islet differentiation and pancreas from childhood and adult donors for comparison. We delineate major cell types, define their regulomes, and describe spatiotemporal gene regulatory relationships between transcription factors. CDX2 emerged as a regulator of enterochromaffin-like cells, which we show resemble a transient, previously unrecognized, serotonin-producing pre-β cell population in fetal pancreas, arguing against a proposed non-pancreatic origin. Furthermore, we observe insufficient activation of signal-dependent transcriptional programs during in vitro β cell maturation and identify sex hormones as drivers of β cell proliferation in childhood. Altogether, our analysis provides a comprehensive understanding of cell fate acquisition in stem-cell-derived islets and a framework for manipulating cell identities and maturity
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Understanding cell fate acquisition in stem-cell-derived pancreatic islets using single-cell multiome-inferred regulomes
Pancreatic islet cells derived from human pluripotent stem cells hold great promise for modeling and treating diabetes. Differences between stem-cell-derived and primary islets remain, but molecular insights to inform improvements are limited. Here, we acquire single-cell transcriptomes and accessible chromatin profiles during in vitro islet differentiation and pancreas from childhood and adult donors for comparison. We delineate major cell types, define their regulomes, and describe spatiotemporal gene regulatory relationships between transcription factors. CDX2 emerged as a regulator of enterochromaffin-like cells, which we show resemble a transient, previously unrecognized, serotonin-producing pre-β cell population in fetal pancreas, arguing against a proposed non-pancreatic origin. Furthermore, we observe insufficient activation of signal-dependent transcriptional programs during in vitro β cell maturation and identify sex hormones as drivers of β cell proliferation in childhood. Altogether, our analysis provides a comprehensive understanding of cell fate acquisition in stem-cell-derived islets and a framework for manipulating cell identities and maturity