Protein-Coated Magnetic Nanoparticles: Creation and Investigation

A novel universal approach to cross-linking of protein macromolecules on the surface of magnetite na-noparticles has been developed. The approach is based on protein liability to free radical modification, leading to the formation of intermolecular covalent cross links. Free radicals are locally generated on the surface of nanoparticles. Using a set of physicochemical methods, it has been proven that stable coatings composed of protein macromolecules are formed around individual nanoparticles. The proteins fixed on nanoparticles do not lose their activity as a result of adsorption and free radical modification. Fluorescent probe approach for evaluation of the native functional properties of serum albumin as a part of coating is suggested. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3537