19 research outputs found
Regional dissemination of Salmonella enterica serovar Enteritidis is season dependent
ObjectiveTo carry out epidemiological typing of clinical isolates of Salmonella enterica serovar Enteritidis by pulsed-field gel electrophoresis (PFGE), random amplified polymorphic DNA (RAPD) and analysis of their antibiotic resistance.MethodsOver a 12-month period, 44 Salmonella Enteritidis isolates, recovered from 40 patients admitted to the University Hospital Center of Amiens, France and from three outpatients, were characterized by the analysis of phenotypic and genotypic traits and clinical data from medical reports.ResultsForty nontyphoidal salmonellosis episodes were diagnosed in hospitalized patients (34 episodes of gastroenteritis, two episodes of bacteremia not affecting other organs, one episodes of bacteremia plus urinary infection, one episodes of bacteremia plus gastroenteritis, one episodes of chronic colitis plus gastroenteritis and one episode of peritonitis), and three carriers were observed in outpatients. By means of PFGE, RAPD and antibiotic susceptibility patterns 44 isolates were subdivided into 16 clonally related groups. Two of them were predominantly implicated in the course of these infections, being responsible for two successive waves of infection, while the others were encountered sporadically
Subconjunctival zoonotic Onchocerca in an Albanian man
A case of subconjunctival infection with a zoonotic species of
Onchocerca is described, in a 16-year-old Albanian man who had
immigrated to Greece. This is the first report of human infection with
Onchocerca in this tissue location and only the eighth report of
zoonotic Onchocerca in man
First report of ampicillin and glycopeptide resistant Enterococcus faecium VanA bacteraemia in Greece
Pneumococcal acute otitis media in children
Objectives To evaluate the clinical and laboratory findings of
Streptococcus pneumoniae acute otitis media in children during a 1 year
period.
Methods From October 1995 to September 1996, 113 children aged 2 months
to 14 years (median 18 months), with S. pneumoniae acute otitis media
were studied. Susceptibility testing was performed by the Kirby-Bauer
method and the E-test, and serotyping by the Quellung reaction.
Results E-test assays detected five Isolates (4.4%) to be highly
resistant to penicillin and 13 (11.5%) that had intermediate
resistance. All isolates were found to be susceptible to vancomycin,
rifampicin and cefotaxime. In total, 25 isolates (22.1%) were resistant
to one or more drugs. Fifty per cent of the penicillin-resistant or
intermediately resistant S. pneumoniae isolates were resistant to
multiple drugs, whereas only 2.1% of the penicillin-susceptible
isolates were resistant to multiple drugs. The predominating serogroups
of the isolates with reduced susceptibility to penicillin were the 19
(61.1%), 9 (16.7%), 23 (11.1%), 6 (5.5%) and 14 (5.5%) whereas
those of the susceptible isolates were the 19 (26.3%), 14 (13.7%), 3
(11.6%), 6 (11.6%), 9 (8.4%), 1 (5.3%) and 12 (5.3%).
Conclusions Streptococcus pneumoniae isolates from children with acute
otitis media were penicillin-insensitive in 15.9%. The multiresistant
S. pneumoniae isolates belonged to serogroups: 19 (45.4%), 9 (27.3%),
6 (18.2%) and 23 (9.1%)
ANTIBIOTIC SENSITIVITIES OF NEISSERIA-MENINGITIDIS ISOLATES FROM PATIENTS AND CARRIERS IN GREECE
Usage of antibiotics in southern Europe is less well regulated than in
northern countries. The proportion (48%) of meningococci in Spain
insensitive to penicillin (MIC greater-than-or-equal-to 0.1 mg/l)
prompted this investigation of antibiotic sensitivities of isolates from
Greek patients with meningitis (31) and carriers (47 school-children and
472 recruits). The agar dilution method was used to determine MIC to
penicillin G (PN), sulphamethoxazole (SU), rifampicin (RF), cefaclor
(CF) and ciprofloxacin (CP).
The proportion of isolates insensitive to PN was 48% for isolates from
patients. 19% from school-children and 36.6% from recruits. Resistance
to SU (MIC greater-than-or-equal-to 16 mg/l) was found in 16% of those
from patients, 10.6% from children and 40% from recruits. None of the
isolates from patients was resistant to RF (greater-than-or-equal-to 1
mg/l) but 6% of those from carriers were. Resistance to CF
(greater-than-or-equal-to 4 mg/l) was found in 9.2% of patient
isolates, 6.4% from children and 23.7% from recruits. All isolates
except one were sensitive to CP (MIC range < 0.0015-0.125 mg/l).
Resistances to PN, SU and RF were analysed by serogroup, serotype and
subtype of the bacteria. The proportion of resistant isolates showed
some variation between different areas of Greece, but it was not
statistically significant
First report of ampicillin and glycopeptide resistant Enterococcus faecium VanA bacteraemia in Greece
Serotyping and antibiotic resistance of Streptococcus pneumoniae isolated from pediatric infections in central Greece
Objective: To determine the distribution of serogroups/serotypes and antibiotic resistance pattern of Streptococcus pneumoniae isolated from pediatric infections in central Greece. Methods: In total, 306 S. pneumoniae strains isolated from children, aged from 18 days to 14 years (median 18 months), during a 21-month period, from different specimen sources, were studied. Susceptibility testing was carried out by the Kirby-Bauer method and by the Etest, and serotyping by the Quellung reaction. Results: Of the S. pneumoniae isolates, 3.9% were highly resistant to penicillin (PR), while 17.6% were intermediately resistant (IPR). PR and IPR isolates were found to be, in general, more resistant to other antibiotics than penicillin-susceptible isolates. The PR and IPR isolates belonged to the serogroup/serotypes 19, 23, 9, 6 and 14 (in descending order of frequency). The penicillin-susceptible isolates belonged to 20 different groups/serotypes, the most common being 19, 6, 14, 9, 3, 23 and 1 (in descending order of frequency). Serogroup 23 was often found to be multiresistant. Conclusions: Resistance to penicillin in S. pneumoniae isolates is relatively low and differs according to the specimen type. All the pneumococcal serogroups/serotypes isolated from the children were found to be included in the 23-valent polysaccharide vaccine. Most of the children with a pneumococcal infection, however, were less than 2 years old and could not be protected by the existing vaccine