<i>In vitro</i> assays of radiolabeled anti-HER3 antibody Mab#58.

<p>(A) Cell binding assay for [⁸⁹Zr]Mab#58 comparing HER3 overexpressing HER3/RH7777 cells (closed triangles) and parent RH7777 cells (closed circles). (B) Competitive inhibition assay for DF-conjugated (closed circles) and unconjugated Mab#58 (closed squares). (C) Internalization assay for [⁸⁹Zr]Mab#58 at 37°C. Changes in the percentage of total radioactivity for each fraction are plotted against incubation time at 37°C (closed circles, internalized fraction; open triangles, membrane-bound fraction; open circles, protein-bound fraction in the culture medium; closed triangles, non-protein-bound fraction in the culture medium). These assays were conducted in duplicate. Data represent the mean.</p

CTOS growth in various culture conditions.

<p>(A) Growth curve of CTOS cultured in StemPro hESC (Invitrogen) or basal medium containing one of the growth factors as indicated. (B) Representative images of CTOS at day 14 in (A).</p

Representative PET images of mice bearing CTOS C45 xenografts.

<p>Serial PET images of mice bearing C45 xenograft (white arrow) injected with [⁸⁹Zr]Mab#58 (upper panels) or similarly ⁸⁹Zr-labeled control IgG.</p

Representative immunohistochemical staining of HER3/RH7777 (A) and RH7777 (B) xenografts for HER3 expression.

<p>Representative immunohistochemical staining of HER3/RH7777 (A) and RH7777 (B) xenografts for HER3 expression.</p

Representative PET images of mice bearing both HER3 overexpressing HER3/RH7777 and parent RH7777 xenografts.

<p>Serial PET images (maximum-intensity-projection (MIP)) of a mouse bearing HER3/RH7777 (right thigh, white arrow) and RH7777 (left thigh, orange arrow) tumors at days 1, 2, 4, and 6 after an intravenous injection of 3.7 MBq [⁸⁹Zr]Mab#58.</p

Representative immunohistochemical staining of CTOS C45 xenograft for HER3 expression.

<p>Representative immunohistochemical staining of CTOS C45 xenograft for HER3 expression.</p