9 research outputs found
Π£ΡΠ°ΡΡΠΈΠ΅ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΉ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² Π² ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Π·Π°ΡΠΈΡΡ ΠΎΡ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ
Immunization of mice by synthetic peptides fragments of meningococcus external membrane fibers caused the expressed protection of animals against infection caused by live virulent meningococcus culture serogroup A, B and C. There are leading part T-lymphocytes in protection of mice from this infection here. The analysis of T-cellular populations by means of a method flowing cytophluorometrics has shown increase CD4+ and CD8+ lymphocytes without change of parity CD4+/CD8+. The increase in number of these lymphocytes occurred in late terms after immunization of mice.Π¦Π΅Π»ΡΡ Π½Π°ΡΡΠΎΡΡΠ΅Π³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠ»ΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΉ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ², ΠΏΡΠΈΠ½ΠΈΠΌΠ°ΡΡΠΈΡ
ΡΡΠ°ΡΡΠΈΠ΅ Π² ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΈΠΌΠΌΡΠ½ΠΈΡΠ΅ΡΠ° ΠΏΡΠΎΡΠΈΠ² ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ ΠΏΡΠΈ ΠΈΠΌΠΌΡΠ½ΠΈΠ·Π°ΡΠΈΠΈ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΠΌΠΈ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ°ΠΌΠΈ Π±Π΅Π»ΠΊΠΎΠ² Π½Π°ΡΡΠΆΠ½ΠΎΠΉ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Ρ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠ° ΡΠ΅ΡΠΎΠ³ΡΡΠΏΠΏΡ Π. ΠΠΌΠΌΡΠ½ΠΈΠ·Π°ΡΠΈΡ ΠΌΡΡΠ΅ΠΉ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΠΌΠΈ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ°ΠΌΠΈ Π±Π΅Π»ΠΊΠΎΠ² Π½Π°ΡΡΠΆΠ½ΠΎΠΉ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Ρ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠ° Π²ΡΠ·ΡΠ²Π°Π»Π° Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΡ Π·Π°ΡΠΈΡΡ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΠΎΡ Π·Π°ΡΠ°ΠΆΠ΅Π½ΠΈΡ ΠΆΠΈΠ²ΠΎΠΉ Π²ΠΈΡΡΠ»Π΅Π½ΡΠ½ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΠΎΠΉ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ² ΡΠ΅ΡΠΎΠ³ΡΡΠΏΠΏ Π, Π ΠΈ Π‘. ΠΠΎΠΊΠ°Π·Π°Π½Π° Π²Π΅Π΄ΡΡΠ°Ρ ΡΠΎΠ»Ρ Π’-Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² Π² Π·Π°ΡΠΈΡΠ΅ ΠΌΡΡΠ΅ΠΉ ΠΎΡ ΡΡΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ. ΠΠ½Π°Π»ΠΈΠ· Π’-ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΉ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΡΠ»ΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΠΈ ΠΏΠΎΠΊΠ°Π·Π°Π» ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠΈΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ CD4+ ΠΈ CD8+ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² Π½Π° ΡΠ°Π·Π½ΡΡ
ΡΡΠ°ΠΏΠ°Ρ
ΠΈΠΌΠΌΡΠ½ΠΎΠ³Π΅Π½Π΅Π·Π° Π±Π΅Π· ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ CD4+/CD8+. Π£Π²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΠΈΡΠ»Π° ΡΡΠΈΡ
Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΠΈΠ»ΠΎ Π² ΠΏΠΎΠ·Π΄Π½ΠΈΠ΅ ΡΡΠΎΠΊΠΈ ΠΏΠΎΡΠ»Π΅ ΠΈΠΌΠΌΡΠ½ΠΈΠ·Π°ΡΠΈΠΈ ΠΌΡΡΠ΅ΠΉ. ΠΠ°Π½Π½ΡΠ΅ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ ΡΠΊΠ°Π·ΡΠ²Π°ΡΡ Π½Π° ΡΠΎΠ»Ρ PorA Π°Π½ΡΠΈΠ³Π΅Π½Π° Π² ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Π’-ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ°. Π’Π΅ΠΌ Π½Π΅ ΠΌΠ΅Π½Π΅Π΅ ΡΠΈΠΏ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ° Π½Π° Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΠΉ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΈΡ Π΄ΠΎ ΡΠΈΡ
ΠΏΠΎΡ Π΄ΠΎ ΠΊΠΎΠ½ΡΠ° Π½Π΅ ΡΡΠ΅Π½
ΠΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²Π°Ρ ΠΏΠΎΠ»ΠΈΠ²Π°ΠΊΡΠΈΠ½Π° Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΎΡΠΈΡΠ΅Π½Π½ΠΎΠΉ IgAI ΠΏΡΠΎΡΠ΅Π°Π·Ρ
IgA1-protease allocated from the culture N. meningitidis serogroup A. As original materials were used three different intermediate products of vaccine production: cultural fluid, cetavlon supernatant and cetavlon precipitate. IgA1-protease was used to evaluate their protectivity and immunogenity. It was shown, that isolated IgA1 protease from the meningococcus serogroup A is able to protect mice, infected by meningococcus serogroup B.IgA1 ΠΏΡΠΎΡΠ΅Π°Π·Π°, Π²ΡΠ΄Π΅Π»Π΅Π½Π½Π°Ρ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ Ρ
ΠΈΠΌΠΈΠΈ ΠΈΠ· ΠΏΡΠΎΠΌΠ΅ΠΆΡΡΠΎΡΠ½ΡΡ
ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² ΠΎΡΠΈΡΡΠΊΠΈ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΠΎΠΉ ΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄Π½ΠΎΠΉ ΡΠ΅ΡΠΎΠ³ΡΡΠΏΠΏΡ Π Π²Π°ΠΊΡΠΈΠ½Ρ, ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠΉ ΠΈΠΌΠΌΡΠ½ΠΎΠ³Π΅Π½Π½ΠΎΠΉ ΠΈ ΠΏΡΠΎΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ Π΄Π»Ρ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΌΡΡΠ΅ΠΉ, ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°Ρ ΠΈΡ
Π·Π°ΡΠΈΡΡ ΠΎΡ ΡΠΌΠ΅ΡΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ Π·Π°ΡΠ°ΠΆΠ΅Π½ΠΈΡ Π²ΠΈΡΡΠ»Π΅Π½ΡΠ½ΡΠΌΠΈ ΡΡΠ°ΠΌΠΌΠ°ΠΌΠΈ ΡΠ΅ΡΠΎΠ³ΡΡΠΏΠΏ Π ΠΈ Π ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ². ΠΡΠΎΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΉ ΡΡΡΠ΅ΠΊΡ ΠΈ Π½Π°ΡΠ°ΡΡΠ°Π½ΠΈΠ΅ Π°Π½ΡΠΈ-IgA1 Π°Π½ΡΠΈΡΠ΅Π» ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ ΠΏΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°ΡΡ Π²Π°ΠΊΡΠΈΠ½Π½ΡΠ΅ ΠΏΠΎΡΠ΅Π½ΡΠΈΠΈ ΠΈΠ·ΡΡΠ°Π΅ΠΌΠΎΠ³ΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°, ΠΊΠΎΡΠΎΡΡΠΉ ΠΌΠΎΠΆΠ΅Ρ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ, Π² ΠΏΠ΅ΡΠ²ΡΡ ΠΎΡΠ΅ΡΠ΅Π΄Ρ, Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΊΠ°Π½Π΄ΠΈΠ΄Π°ΡΠ° ΠΎΡΡΡΡΡΡΠ²ΡΡΡΠ΅ΠΉ Π΄ΠΎ Π½Π°ΡΡΠΎΡΡΠ΅Π³ΠΎ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΠΎΠΉ ΡΠ΅ΡΠΎΠ³ΡΡΠΏΠΏΡ Π Π²Π°ΠΊΡΠΈΠ½Ρ
ΠΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΡΠ΅ Π²Π°ΠΊΡΠΈΠ½Ρ: ΠΎΡ ΠΊΠ°ΠΏΡΡΠ»ΡΠ½ΡΡ ΠΏΠΎΠ»ΠΈΡΠ°Ρ Π°ΡΠΈΠ΄ΠΎΠ² Π΄ΠΎ ΠΏΡΠΎΡΠ΅Π°Π·
Microbial capsular polysaccharides for many years provided a highly practical public health vaccines for preventing meningococcal, pneumococcal and Haemophilus influenza infection, and typhoid fever. Their application in the form of conjugates with protein carriers eliminate the gap in protection against these infections in children under one year. Extremely promising turned out offered us a new generation of vaccines, which have synthetic peptides conjugated to a meningococcal polysaccharide. Thus, new approaches to the solution of the problem of meningococcal disease vaccination serogroup B were open. In recent years, Russian researchers first suggested to use IgA1 protease (one of the major virulence factors of microbes and almost identical for mentioned below infections) for prevention of such diseases as meningococcal of all serogroups, pneumococcus and hemophilia infections. Patented processes for producing of the vaccine define domestic priority of its production and use.ΠΠΈΠΊΡΠΎΠ±Π½ΡΠ΅ ΠΊΠ°ΠΏΡΡΠ»ΡΠ½ΡΠ΅ ΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄Ρ Π½Π° ΠΏΡΠΎΡΡΠΆΠ΅Π½ΠΈΠΈ ΠΌΠ½ΠΎΠ³ΠΈΡ
Π»Π΅Ρ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°Π»ΠΈ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π·Π΄ΡΠ°Π²ΠΎΠΎΡ
ΡΠ°Π½Π΅Π½ΠΈΠ΅ Π²ΡΡΠΎΠΊΠΎΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌΠΈ Π²Π°ΠΊΡΠΈΠ½Π°ΠΌΠΈ Π΄Π»Ρ ΠΏΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΠΊΠΈ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΡΡ
, ΠΏΠ½Π΅Π²ΠΌΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΡΡ
, Π³Π΅ΠΌΠΎΡΠΈΠ»ΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ ΠΈ Π±ΡΡΡΠ½ΠΎΠ³ΠΎ ΡΠΈΡΠ°. Π ΠΈΡ
ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π² Π²ΠΈΠ΄Π΅ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΎΠ² Ρ Π±Π΅Π»ΠΊΠΎΠ²ΡΠΌΠΈ Π½ΠΎΡΠΈΡΠ΅Π»ΡΠΌΠΈ Π»ΠΈΠΊΠ²ΠΈΠ΄ΠΈΡΠΎΠ²Π°Π»ΠΎ ΠΏΡΠΎΠ±Π΅Π» ΠΏΡΠΈ Π·Π°ΡΠΈΡΠ΅ ΠΎΡ ΡΡΠΈΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ Π΄Π΅ΡΠ΅ΠΉ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ Π΄ΠΎ ΠΎΠ΄Π½ΠΎΠ³ΠΎ Π³ΠΎΠ΄Π°. ΠΠ΅ΠΎΠ±ΡΡΠ°ΠΉΠ½ΠΎ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ ΠΎΠΊΠ°Π·Π°Π»ΠΎΡΡ ΠΏΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½Π½ΠΎΠ΅ Π½Π°ΠΌΠΈ Π½ΠΎΠ²ΠΎΠ΅ ΠΏΠΎΠΊΠΎΠ»Π΅Π½ΠΈΠ΅ Π²Π°ΠΊΡΠΈΠ½, Π³Π΄Π΅ ΡΠΆΠ΅ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠ΅ΠΏΡΠΈΠ΄Ρ ΠΊΠΎΠ½ΡΡΠ³ΠΈΡΠΎΠ²Π°Π»ΠΈΡΡ Ρ ΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄Π°ΠΌΠΈ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ². Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, Π±ΡΠ»ΠΈ ΠΎΡΠΊΡΡΡΡ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Ρ ΠΊ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΠΏΡΠΎΠ±Π»Π΅ΠΌΡ Π²Π°ΠΊΡΠΈΠ½ΠΎΠΏΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΠΊΠΈ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ ΡΠ΅ΡΠΎΠ³ΡΡΠΏΠΏΡ Π. Π ΠΏΠΎΡΠ»Π΅Π΄Π½ΠΈΠ΅ Π³ΠΎΠ΄Ρ ΠΎΡΠ΅ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΠΈ Π²ΠΏΠ΅ΡΠ²ΡΠ΅ ΠΏΡΠ΅Π΄Π»ΠΎΠΆΠΈΠ»ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°ΡΡ Π΄Π»Ρ ΠΏΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΠΊΠΈ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ Π²ΡΠ΅Ρ
ΡΠ΅ΡΠΎΠ³ΡΡΠΏΠΏ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ, ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Π½ΡΡ
ΠΏΠ½Π΅Π²ΠΌΠΎΠΊΠΎΠΊΠΊΠ°ΠΌΠΈ ΠΈ Π³Π΅ΠΌΠΎΡΠΈΠ»Π°ΠΌΠΈ, ΠΎΠ΄ΠΈΠ½ ΠΈΠ· Π³Π»Π°Π²Π½ΡΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ² Π²ΠΈΡΡΠ»Π΅Π½ΡΠ½ΠΎΡΡΠΈ ΡΡΠΈΡ
ΠΌΠΈΠΊΡΠΎΠ±ΠΎΠ² β IgA1 ΠΏΡΠΎΡΠ΅Π°Π·Ρ, ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ ΠΈΠ΄Π΅Π½ΡΠΈΡΠ½ΡΡ Π΄Π»Ρ Π²ΡΠ΅ΠΉ ΡΠΊΠ°Π·Π°Π½Π½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ. ΠΠ°ΠΏΠ°ΡΠ΅Π½ΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΡΠΏΠΎΡΠΎΠ±Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΡΡΠΎΠΉ Π²Π°ΠΊΡΠΈΠ½Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡ ΠΎΡΠ΅ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΉ ΠΏΡΠΈΠΎΡΠΈΡΠ΅Ρ Π΅Π΅ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Π° ΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ
Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins
Using the genome sequence of IgA1 protease of N. meningitidis of serogroup B, four recombinant proteins of different structure and molecular weight were constructed. These proteins were equal in inducing the formation of specific antibodies to IgA1 protease and had protective properties against meningococci. In the sera of immunized mice, anti-IgA1 protease antibodies were detected by whole-cell ELISA, which indicated the presence of IgA1 protease on the surface of these bacteria. We hypothesized that the protective properties of IgA1 protease-based antigens and IgA1 protease analogs could be realized not only via impairment of bacterium adhesion to the mucosa, but also via suppression of this pathogen in the organism. The presented findings seem promising for using these proteins as the basis for anti-meningococcus vaccine. Β© 2016, Springer Science+Business Media New York
Induction of antimeningitis immunity by the synthetic peptides: I. The immunoactive synthetic fragments of porin A from Neisseria meningitidis
Fourteen peptides corresponding to sequences of all the exposed and some of the transmembrane protein regions of porin A from the outer membrane of Neisseria meningitidis strain B: 15:P1.7,16 were synthesized. Mice of various lines were immunized with the free peptides not conjugated with any protein carrier. It was shown that the majority of the peptides possess immunogenic properties. Two peptides were identified binding to antibodies present in the serum of mice after meningitis. Protective properties of a number of the synthesized peptides were studied, and three peptide sequences inducing mice protection to an experimental infection with N. meningitidis were identified. Β© 2000 MAIK "Nauka/Interperiodica"
Induction of antimeningitis immunity by the synthetic peptides. I. The immunoactive synthetic fragments of porin a from Neisseria meningitidis
Fourteen peptides corresponding to sequences of all the exposed and some of the transmembrane protein regions of porin A from the outer membrane of Neisseria meningitidis strain B:15:P1.7,16 were synthesized. Mice of various lines were immunized with the free peptides not conjugated with any protein carrier. It was shown that the majority of the peptides possess immunogenic properties. Two peptides were identified binding to antibodies present in the serum of mice after meningitis. Protective properties of a number of the synthesized peptides were studied, and three peptide sequences inducing mice protection from an experimental infection with N. meningitidis were identified
Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins
Using the genome sequence of IgA1 protease of N. meningitidis of serogroup B, four recombinant proteins of different structure and molecular weight were constructed. These proteins were equal in inducing the formation of specific antibodies to IgA1 protease and had protective properties against meningococci. In the sera of immunized mice, anti-IgA1 protease antibodies were detected by whole-cell ELISA, which indicated the presence of IgA1 protease on the surface of these bacteria. We hypothesized that the protective properties of IgA1 protease-based antigens and IgA1 protease analogs could be realized not only via impairment of bacterium adhesion to the mucosa, but also via suppression of this pathogen in the organism. The presented findings seem promising for using these proteins as the basis for anti-meningococcus vaccine. Β© 2016, Springer Science+Business Media New York
Induction of antimeningitis immunity by the synthetic peptides. I. The immunoactive synthetic fragments of porin a from Neisseria meningitidis
Fourteen peptides corresponding to sequences of all the exposed and some of the transmembrane protein regions of porin A from the outer membrane of Neisseria meningitidis strain B:15:P1.7,16 were synthesized. Mice of various lines were immunized with the free peptides not conjugated with any protein carrier. It was shown that the majority of the peptides possess immunogenic properties. Two peptides were identified binding to antibodies present in the serum of mice after meningitis. Protective properties of a number of the synthesized peptides were studied, and three peptide sequences inducing mice protection from an experimental infection with N. meningitidis were identified