Representative B-scan images of the porcine sclera.

<p>OCT intensity image (a), phase retardation image (b) birefringence image (c), DOAU images (d) and (e) are shown. In figure (e), red color indicates the region above the threshold of DOAU. Scale bars show 250 m.</p

OCT image with a rubber plate for the measurement of the cross-sectional area.

<p>Since the edges of the rubber plate at left and right sides of the sclera correspond to the edges of a back surface of the sclera, the manually-segmented region indicated by the dashed curve shows the cross-sectional area of the sample in air. Scale bars show 250 m.</p

The experimental system setup for the birefringence and elasticity measurements.

<p>The experimental system setup for the birefringence and elasticity measurements.</p

Porcine sclera dissected by scalpel blades.

<p>Strips in the lower and upper sides of the figure are peripapillary sclera and sclera near the limbus, respectively. The four strips of sclera between them were used for the measurement.</p

Correlation coefficients () and -values between birefringence and elastic parameters at each position.

<p>Statistically significant correlations are marked with *, **, and *** for 0.05, 0.01, and 0.001, respectively.</p

Transferases increased in h1299/CD82 cells (Expression levels of possible regulators of sialyl Lewis antigens analysed by DNA microarray).

<p>Transferases increased in h1299/CD82 cells (Expression levels of possible regulators of sialyl Lewis antigens analysed by DNA microarray).</p

Effects of CD82 on the protein expression of glycosyltransferases related to sialyl Lewis synthesis.

<p>Whole cell lysates (300 μg) of h1299 cells (h1299/zeo, zeo; h1299/CD82, CD82; h1299/CD82-sh.control, sh.cont; h1299/CD82-sh.CD82, sh.CD82) were resolved by 10% SDS-PAGE and analysed by immunoblotting with anti-ST3GAL primary antibodies. The same blots were stripped and re-probed for β-actin and CD82. Experiments were repeated in triplicate, and the most representative data are shown.</p

Effects of CD82 on the expression of genes encoding glycosyltransferases (ST3GALs) related to sialyl Lewis synthesis.

<p>Total RNA was isolated from h1299 cells and analysed by real-time RT-PCR. mRNA levels of glycosyltransferase-encoding genes <i>(ST3GALs)</i> were corrected relative to the levels of <i>GAPDH</i> mRNA, with h1299/zeo set at 1. Data are shown as the mean ± SEM.</p

Effects of CD82 on the expression of sialyl Lewis antigens.

<p>Whole cell lysates (200 μg) of h1299 cells (h1299/zeo, zeo; h1299/CD82, CD82; h1299/CD82-sh.control, sh.cont; h1299/CD82-sh.CD82, sh.CD82) were resolved by 7.5% SDS-PAGE and analysed by immunoblotting with anti-sLe^a (A) or anti-sLe^x (B) antibodies. The same blots were stripped and re-probed for β-actin as a loading control. Experiments were repeated in triplicate, and the most representative data are shown.</p

Inhibitory effect of CD82 on cancer cell metastasis in a mouse metastasis model.

<p>The tail veins of nude mice were injected with 1.0 × 10⁶ h1299/zeo or h1299/CD82 cells. Eight weeks after injection, the mice were killed and the lungs recovered. Metastatic foci were counted by eye. The injection of h1299/zeo cells resulted in 100% lung metastasis (7/7), whereas h1299/CD82 cells showed a significantly lower rate of metastasis (28.5%; 2/7).</p