1 research outputs found
Selection of the root endophyte Pseudomonas brassicacearum CDVBN10 as plant growth promoter for Brassica napus L. crops
[EN]Rapeseed (Brassica napus L.) is an important crop worldwide, due to its multiple uses,
such as a human food, animal feed and a bioenergetic crop. Traditionally, its cultivation is based on
the use of chemical fertilizers, known to lead to several negative e ects on human health and the
environment. Plant growth-promoting bacteria may be used to reduce the need for chemical fertilizers,
but e cient bacteria in controlled conditions frequently fail when applied to the fields. Bacterial
endophytes, protected from the rhizospheric competitors and extreme environmental conditions,
could overcome those problems and successfully promote the crops under field conditions. Here,
we present a screening process among rapeseed bacterial endophytes to search for an e cient
bacterial strain, which could be developed as an inoculant to biofertilize rapeseed crops. Based on
in vitro, in planta, and in silico tests, we selected the strain Pseudomonas brassicacearum CDVBN10
as a promising candidate; this strain produces siderophores, solubilizes P, synthesizes cellulose
and promotes plant height in 5 and 15 days-post-inoculation seedlings. The inoculation of strain
CDVBN10 in a field trial with no addition of fertilizers showed significant improvements in pod
numbers, pod dry weight and shoot dry weight. In addition, metagenome analysis of root endophytic
bacterial communities of plants from this field trial indicated no alteration of the plant root bacterial
microbiome; considering that the root microbiome plays an important role in plant fitness and
development, we suggest this maintenance of the plant and its bacterial microbiome homeostasis
as a positive result. Thus, Pseudomonas brassicacearum CDVBN10 seems to be a good biofertilizer to
improve canola crops with no addition of chemical fertilizers; this the first study in which a plant
growth-promoting (PGP) inoculant specifically designed for rapeseed crops significantly improves
this crop’s yields in field conditions