Carcinoma of the parathyroid gland: a case report

Carcinoma of the parathyroid gland causes 0.005% of all malignancies. Although the etiology remains unknown, possible contributing factors include neck radiation, chronic secondary hyperparathyroidism due to kidney failure, and vitamin D deficiencies. It occurs in syndromes such ahyperparathyroidism-jaw tumor syndrome, multiple endocrine neoplasia types 1 and 2A, and familial isolated hyperparathyroidism

UTICAJ PRAVA EVROPSKE UNIJE NA NOVO MEĐUNARODNO PRIVATNO PRAVO CRNE GORE SA POSEBNIM OSVRTOM NA OBLAST MJERODAVNOG PRAVA

Commitment to perform unification of the relevant EU law by adopting regulations in a significant number of areas of private international law entitles opinions of Europeanisation of private international law. The impact of this important legislative action at EU level was not without repercussions in the countries candidates for EU membership, a characteristic example being Montenegro and its draft law on Private International Law. Given the dynamics of integration processes and the fact that it might take many years for Montenegro to achieve full EU membership, the new codification of private international law of Montenegro took a differentiated approach to the EU legislation. Thus, in every case regard is had of the EU legislation, but at this very moment only those enactments which are considered to be of ultimate national interest are subject of reception and adjustment in order to become part of a national law. Thus, the reception has been made of the Rome I and the Rome II Regulations provisions (for contractual and non-contractual obligations), with necessary adjustments, as well as the provisions of the Hague Protocol on the law applicable to maintenance obligations. In the area of the law of inheritance, the starting point was the Regulation on inheritance. Unlike the above, in terms of rules determining the applicable law relating to the other fields account is taken of the new developments in the European Union and internationally, but primarily on national interests and needs of the national legal tradition and legal certainty. Three important concepts, deeply rooted in European Union law are underlying principles of the new codification of Private International Law of Montenegro: the overriding mandatory provisions, the concept of habitual residence, and a significant expansion in scope of conflicts of law autonomy.Predlogom novog Zakona o međunarodnom privatnom pravu izvršena je recepcija pravila Uredbi Rim I i Rim II (za ugovorne i vanugovorne obaveze), uz neophodna prilagođavanja, a takođe su preuzete odredbe Haškog protokola o jerodavnom pravu za obaveze izdržavanja. U domenu mjerodavnog prava za nasljeđivanje, polazna osnova su bila rješenja Predloga uredbe o nasljeđivanju. Za razliku od navedenog, u pogledu pravila o određivanju mjerodavnog prava za druge odnose vođeno je računa o novim tendencijama u pravu Evropske unije, kao i na međunarodnom planu, ali prevashodno o nacionalnim interesima, pravnoj tradiciji i potrebama pravne sigurnosti. Tri značajna koncepta, duboko utemeljena u pravo Evropske unije su detaljno razrađena u novoj kodifikaciji međunarodnog privatnog prava Crne Gore i predstavljaju osnov modernizacije nacionalnog zakonodavstva: norme neposredne primjene, koncept uobičajenog boravišta i značajno proširenje polja primjene kolizionopravne autonomije volje stranaka

Liver damage caused by hepatitis C viral infection and ethyl alcohol consumption

Background/Aim. Hepatitis C virus infection (HCV) is a complex disease, most commonly chronicle (80-85%). The aim of this research was to determinate the level of the liver damage in the patients cansed by HCV in conjunction with consuming ethyl alcohol. Methods. The research included 15 patients with chronic HCV infection supported by the misuse of ethyl alcohol, as well. The diagnosis of C infection hepatitis was proved using the ELISA test and PCR method. Results. The results of the study showed the liver damage by both HCV infection and ethyl alcohol, which was verified by the presence of biochemical changes and patohystological processing of the patients (liver biopsy and prosection). Patohystological changes were at the level of liver cirrhosis and carcinoma (2 patients). There was a signficant difference between the two subgroups (p < 0.001) regarding the examined values γ-GT, PLT and PTV. The basic therapeutic procedure was to introduce this category of patients into alcohol abstinence, and, in a few patients, to apply the antivirus therapy, as well. Conclusion. Based on the number of the examined patients (n = 15), we could conclude that a prolonged ethyl alcohol misuse with the presence of HCV infection was in a correlation with the liver disease progression

FOR THOSE WHO FIGHT, FALL AND RAISE AGAIN - IMPACT OF COVID-19 PANDEMIC ON MENTAL HEALTH OF HEALTH CARE PROVIDERS

Background: During the COVID-19 pandemic health care providers found themselves under increased demands in the work environment and in their professional and personal lives which created both physical and mental health challenges. Thus, we aim to provide an integrative review that identifies and summarizes the research published regarding mental health functioning in health care providers, in Serbia, since the beginning of the pandemic. Subjects and methods: A search of the published literature was conducted using Medline and SCIndex databases, applying key words “COVID-19” and “Serbia”. The search was limited to papers published since the beginning of the COVID-19 pandemic until January 2022. Two reviewers independently screened the retrieved papers. The study used pre-defined inclusion and exclusion criteria. Results: We identified eight papers on the subject of mental health functioning in health care providers. The studies were all original research papers with predominantly cross-sectional study design, using online assessments. Sample size varied in number of participants and profile of medical providers (physicians, nurses and medical technicians, community pharmacists). Dominantly, focus of interests of researchers were exploration of levels of anxiety, depression, sleep disturbances, burnout, as well as behavioural changes and environmental influences. Conclusions: The studies related to mental health of medical professionals show the importance of recognizing the psychological challenges posed by health crises caused by COVID-19. They raise awareness of recognizing differences and difficulties between wide range of medical sectors, and appeal for necessity for accessible and professional psychological support. Further studies should address the detailed exploration of the mental health of this specific population, as well as propose strategies needed to balance the challenges posed by the pandemic

Evaluation of glassionomer cement application for permanent binding of prosthetic dentures

Fixed dentures are created in order to rehabilitate the function of orofacial system, to correct disordered interdental relation, as well as the relation between jaws and to do esthetic correction. Binding of fixed dentures to the teeth carriers is done with a layer of binding biomaterials from the group of dental cements. Cement as the choice of dental biomaterials has to be the best cement for the most frequent, routine management for these type of works and most frequently used materials – metal, acrylate, ceramics. Cement must be safe to use with patients. Ideally, it must have longtime advantages such as to be for various uses, to find the right balance between results and simplicity of application, which are the most important for routine management. There are many types of cement. In this study, Zn-phosphate and glassionomer cement were used. In modern dental technology, production of cement is well developed and the most concerns are about non-toxicity, biocompatibility, bioinertion, biofunction. In some cases acidic balance disorder of saliva medium can bring to release of low doses of heavy metal ions after the reaction of cement and heavy metal ions from dental compounds. In this study, the best results were achieved with glassionomer cement, because the lowest percent of released heavy metal ions in saliva medium was noticed after cementing of fixed dentures by glassionomer cement. Because of its characteristics, glassionomer cement can be the choice material for permanent cementing of fixed prosthetic dentures

HIGH SENSITIVITY C-REACTIVE PROTEIN AS PREDICTION FACTOR OF DISEASE PROGRESSION IN PATIENTS WITH CHRONIC HEPATITIS C AND MILD LIVER STEATOSIS

Chronic hepatitis C (CHC) is a major cause of liver cirrhosis and hepatocellular carcinoma. Steatosis occurs in almost 50% of patients with CHC, who make a faster progression to cirrhosis. The "atypical " patients are registred too, with normal values of blood sugar, cholesterol, triglycerides, BMI, body weight having non-alcoholic steatohepatitis (NASH) and CHC. The hsCRP levels rise before and simultaneously with the chronic hepatitis and cirrhosis progression, therefore, it is a useful prognostic parameter. According to our knowledge, there are no sufficient data concerning hsCRP concentrations in CHC patients, although it predicts or detects different grades of cirrhosis. For that reason, the aim of our research was to assess the hsCRP in patients with CHC.The investigation involved 45 patients (28 males and 17 females), mean age 41±15 years, with CHC, without any accompanying disease. The control group consisted of 45 healthy volunteers (22 males, 23 females), mean age 34±10 years. The CHC patients' group was divided into two subgroups, the first, which consisted of 23 patients with evidenced histological signs of mild steatosis, and the second one, comprising 22 patients without the mentioned signs; hsCRP concentrations were measured in each patients' (sub)group.The findings indicate that the hsCRP value had a statistically significant increase in the CHC patients' group compared to the control group (p<0.05). In the CHC and mild liver steatosis patients subgroup, even more statistically significant hsCRP increase occured compared to the other subgroup (p<0.001).It can be concluded, based on the acquired results, that hsCRP should be considered as a CHC progression prognostic factor, in order to make a well-timed and special therapeutic approach to the CHC individuals even more prone to the disease progression

Genotoksični učinak ekstrakta zelenog čaja na ljudske stanice raka grkljana u kulturi

Green tea (Camellia sinensis) contains several bioactive compounds which protect the cell and prevent tumour development. Phytochemicals in green tea extract (mostly flavonoids) scavenge free radicals, but also induce pro-oxidative reactions in the cell. In this study, we evaluated the potential cytotoxic and prooxidative effects of green tea extract and its two main flavonoid constituents epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) on human laryngeal carcinoma cell line (HEp2) and its crossresistant cell line CK2. The aim was to see if the extract and its two flavonoids could increase the sensitivity of the cisplatin-resistant cell line CK2 in comparison to the parental cell line. The results show that EGCG and green tea extract increased the DNA damage in the CK2 cell line during short exposure. The cytotoxicity of EGCG and ECG increased with the time of incubation. Green tea extract induced lipid peroxidation in the CK2 cell line. The pro-oxidant effect of green tea was determined at concentrations higher than those found in traditionally prepared green tea infusions.Zeleni čaj, koji je vrlo popularno piće, proizvodi se iz biljke Camellia sinensis i bogat je fl avonoidima za koje se smatra da imaju važnu ulogu u sprečavanju nastanka raka. Kao najvažniji mehanizmi djelovanja fl avonoida najčešće se spominju vezanje slobodnih radikala te sprečavanje nastanka reaktivnih kisikovih skupina. Cilj ovog rada bio je istražiti potencijalni genotoksični učinak ekstrakta zelenog čaja te epigalokatehin galata (EGCG) i epikatehin galata (ECG), fl avonoida koji se u zelenom čaju nalaze u najvišoj koncentraciji. Dobiveni su rezultati pokazali da EGCG i ekstrakt zelenog čaja izazivaju povećanu citotoksičnost otporne stanične linije raka grkljana CK2 nakon kraće inkubacije. Produljenjem vremena inkubacije povećava se citotoksičnost istraživanih spojeva. Također, ekstrakt zelenog čaja izaziva lipidnu peroksidaciju u CK2-stanicama. Prooksidativni učinak ekstrakta zelenog čaja u koncentracijama višim od onih prisutnih u infuzijskoj otopini dobivenoj tradicionalnim načinom, imaju prooksidativno djelovanje

ATYPICAL, CHOLESTATIC FORM OF HEPATITIS A WITH AN EXTRAHEPATIC, CUTANEOUS MANIFESTATION – A CASE REPORT

Hepatitis A sometimes can present through atypical forms and merged extrahepatic manifestations. Most atypical forms include relapsing hepatitis and cholestatic hepatitis. Extrahepatic manifestations of HAV are rare, and include evanescent skin rash and transient arthralgias. A 25-year-old male, previously healthy, developed acute hepatitis. Hepatitis A virus was serologically confirmed. No skin efflorescences were seen at the beginning. Over the course of the illness which extended up to fifteen weeks, bilirubin and aminotransferase levels were continuously highly elevated. Nine weeks after the initial presentation he developed a painless, non-pruritic, purpura-like maculopapular erythematous rash over both lower extremities. Five weeks later, the rash had completely disappeared and the patient’s clinical status much improved. This case demonstrates two unusual and unrelated manifestations: prolonged cholestatic form of hepatitis and cutaneous vasculitis. Possible unusual manifestations of this disease should be a reminder of a brief diagnostic examination, which is usually not done in hepatitis A

Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin

Silicon (Si) is the major constituent of soil present in various fractions, i.e., mobile, adsorbed, occluded (in pedogenic oxides and hydroxides), amorphous (biogenic and lithogenic) and crystalline (primary and secondary silicates, and quartz). Different soil factors such as pH, temperatures, microbial activity, the presence of cations, Al/Fe oxides and hydroxides and organic compounds, influence Si transformation, thereby modifying plant availably of Si. Silicon mobility and transformation in the soil have mainly been studied in the context of pedogenesis or biogeochemical Si cycling. However, research on Si mobility, transformation, and plant availability in the rhizosphere is still lacking. Here, we investigated the root potential of white lupine (Lupinus albus L.), known as a phosphorus (P)-efficient model plant (e.g., root release of H+ and carboxylates), to mobilize Si from the soil. Plants were grown in the rhizoboxes filled with low P soil (control) and fertilized with different N-forms (NO3, NH4 and NO3NH4). The control, NO3- and NO3NH4-fertilized plants accumulated significantly lower amounts of Si than the NH4-fertilized ones. All applied N-forms influenced Si availability in the bulk soil, but Si fractions have further been modified in the rhizosphere, what was crucial for Si accumulation in plants. For instance, NO3 supply slightly decreased Si availability in the bulk soil, but lupine plants accumulated a similar amount of Si as the control plants. A strong gradient of decreasing Si concentrations between bulk and rhizosphere soils was observed in mobile, adsorbed, and amorphous biogenic Si pools in the control and in all N treatments, while occluded and lithogenic amorphous Si pools were recalcitrant. Interestingly, a gradient of increasing concentrations of the amorphous biogenic Si pool between bulk and rhizosphere soils was recorded in the NH4 treatment, concomitantly with the strongest rhizosphere acidification

The role of FasR/FasL system in pathogenesis of myeloprolyferative neoplasms

Mijeloproliferativne neoplazije (MPN) su hematološki maligniteti koji se karakterišu nekontrolisanom ćelijskom proliferacijom i poremećajem u procesu apoptoze. Sistem FasR/FasL je uključen u kontrolu apoptoze u različitim tipovima ćelija. U ovom radu je izučavana uloga sistema FasR/FasL u patogenezi mijeloproliferativnih neoplazija. Upoređena je ekspresija FasR i FasL između pacijenata sa MPN (24) i zdravih kontrola korišćenjem metode 'real-time' PCR. Detektovana je povećana ekspresija FasR kod pacijenata sa MPN. Nije utvrđena razlika u ekspresiji FasL. Mutacija B617F u JAK2 genu, karakteristična za MPN, je nađena kod 13 od 24 pacijenta. Pokazano je da ekspresija FasR i FasL nije povezana sa prisustvom B617F JAK2 mutacije.Myeloproliferative neoplasms (MPN) are hematological malignancies characterized by uncontrolled cell proliferation and impaired apoptosis. The FasR/FasL system is involved in the control of apoptosis in different cell types. Here we have investigated the role of FasR/FasL in the pathogenesis of MPNs. We compared FasR/FasL expression between MPN patients (24) and healthy individuals using the real-time PCR assay. We found an increase of FasR expression in MPN patients. No difference was detected in FasL expression. Mutation V617F in the JAK2 gene, a hallmark of MPN, was detected in 13/24 patients. We found that neither FasR nor FasL expression were related to the presence of JAK2 V617F mutation