2 research outputs found
バラの低温耐性遺伝子のクローニング
Rose is a garden species loved all over the world and is designated as “the flower of Maebashi-shi”. The production of roses with wonderful flowers requires to some extent the regulation of temperature. On the other hand, the cost of fuels has gone up in recent years. Therefore, the balance of maintaining high quality and productivity of roses in winter and keeping down the cost of cultivation is a big issue. In this study, we have cloned two cDNA fragments probably related to cold resistance of rose. One fragment (691 bp) encodes a putative cold shock protein (CSP) which is up-regulated by cold stress and functions as an RNA chaperone through the binding to RNA molecules under conditions of low temperature. The other cDNA fragment (524 bp) encodes a putative plant-specific transcription factor, DREB. DREB is stress-inducible transcription factor which induces various stress-resistance genes. In some species such as Arabidopsis and rice, high level of DREB gene expression has conferred augmented stress tolerance on the plant. We expect that regulating the expression of isolated genes through the genetical modification technique will enable us to breed novel roses which are highly resistant to cold stress