5 research outputs found
Rare neurogenic retinal tumors in adults: morphological features and diagnostic challenges
The histological diagnosis of neurogenic tumors remains a challenge,
which may be indicated particularly by the fact that new entities appeared in the new
edition of the World Health organization (WHO) classification.
Purpose: To review the histomorphologic and immunohistochemic features of rare
variants of neurogenic ocular (retinal) tumors in adults.
Material and Methods: Six rare ocular tumors were selected for the study from all
clinical material submitted for pathohistological examination from 2017 to 2020 based
on the presence of morphological evidence of neurogenic differentiation.
Results: The study sample of six rare neurogenic retinal tumors in adults was
conventionally divided into three types: (1) retinal tumors immunohistochemically
similar to cellular ependymoma, but histologically similar to retinoblastoma;
(2) tumors showing no histological pattern characteristic for dictyoma, but the
immunohistochemical features of neuroepithelial differentiation; and (3) tumors showing
histological patterns similar to medulloepithelioma, but the immunohistochemical
features of glial markers.
Conclusion: Obviously, when dividing these tumors into histogenetic groups, not only
the histological structure and immunohistochemical profile, but also tumor location
and typical patient age should be taken into account
ΠΡΡ ΠΈΠ΅ΠΏΠΈΡΠΊΠΎΠΏ, Ρ ΠΈΡΡΡΠ³, ΡΠΈΠ»ΠΎΡΠΎΡ Π±ΠΎΠΆΡΠ΅ΠΉ ΠΌΠΈΠ»ΠΎΡΡΡΡ
ΠΠ°Π»Π΅Π½ΡΠΈΠ½ Π€Π΅Π»ΠΈΠΊΡΠΎΠ²ΠΈΡ ΠΠΎΠΉΠ½ΠΎ-Π―ΡΠ΅Π½Π΅ΡΠΊΠΈΠΉ (Π‘Π²ΡΡΠΈΡΠ΅Π»Ρ ΠΡΠΊΠ°) (1877β1961) β Π°ΡΡ
ΠΈΠ΅ΠΏΠΈΡΠΊΠΎΠΏ Π‘ΠΈΠΌΡΠ΅ΡΠΎΠΏΠΎΠ»ΡΡΠΊΠΈΠΉ ΠΈ ΠΡΡΠΌΡΠΊΠΈΠΉ, Π° Π² ΠΌΠΈΡΡ β Π²ΡΠ΄Π°ΡΡΠΈΠΉΡΡ Ρ
ΠΈΡΡΡΠ³, ΡΠΎΠΏΠΎΠ³ΡΠ°ΡΠΎΠ°Π½Π°ΡΠΎΠΌ ΠΌΠΈΡΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠΎΠ²Π½Ρ, Π΄ΠΎΠΊΡΠΎΡ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½Ρ, ΠΏΡΠΎΡΠ΅ΡΡΠΎΡ. ΠΠ° ΡΡΡΠ΄Ρ ΠΏΠΎ Ρ
ΠΈΡΡΡΠ³ΠΈΠΈ, ΠΊΠΎΡΠΎΡΡΠ΅ ΡΡΠ°Π»ΠΈ Π½Π°ΡΡΠΎΠ»ΡΠ½ΡΠΌΠΈ ΠΊΠ½ΠΈΠ³Π°ΠΌΠΈ ΠΌΠ½ΠΎΠ³ΠΈΡ
ΠΏΠΎΠΊΠΎΠ»Π΅Π½ΠΈΠΉ Π²ΡΠ°ΡΠ΅ΠΉ, Π°ΡΡ
ΠΈΠ΅ΠΏΠΈΡΠΊΠΎΠΏ ΡΡΠ°Π» Π»Π°ΡΡΠ΅Π°ΡΠΎΠΌ Π‘ΡΠ°Π»ΠΈΠ½ΡΠΊΠΎΠΉ (ΠΠΎΡΡΠ΄Π°ΡΡΡΠ²Π΅Π½Π½ΠΎΠΉ) ΠΏΡΠ΅ΠΌΠΈΠΈ I ΡΡΠ΅ΠΏΠ΅Π½ΠΈ.
Π 1999 Π³. Π±ΡΠ» ΠΏΡΠΈΡΠΈΡΠ»Π΅Π½ ΠΊ Π»ΠΈΠΊΡ Π½ΠΎΠ²ΠΎΠΌΡΡΠ΅Π½Π½ΠΈΠΊΠΎΠ² ΠΈ ΠΈΡΠΏΠΎΠ²Π΅Π΄Π½ΠΈΠΊΠΎΠ² Π΄Π»Ρ ΠΎΠ±ΡΠ΅ΡΠ΅ΡΠΊΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΏΠΎΡΠΈΡΠ°Π½ΠΈΡ.
ΠΠ²ΡΠΎΡ ΠΌΠ½ΠΎΠ³ΠΈΡ
ΠΏΡΠΎΠΏΠΎΠ²Π΅Π΄Π΅ΠΉ, ΡΡΠ°ΡΠ΅ΠΉ ΠΈ ΠΊΠ½ΠΈΠ³ Π΄ΡΡ
ΠΎΠ²Π½ΠΎ-ΠΌΠΎΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈ ΠΏΠ°ΡΡΠΈΠΎΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ.V. F. Voyno-Yasenetsky (St. Luke) (1877β1961) is the Archbishop of Simferopol and Crimean regions,
a famous surgeon and topographoanatomist, doctor of medicine, professor. The author of scientific
works on surgery, which are used for constant reference of doctors. The archbishop was a laureate of
the Stalin (State) Prize of the 1st degree.
He was canonized by Orthodox Church in 1999 year. He is the author of numerous preachings, works
and books on scientific, religious, moral and patriotic topics
The influence of immunodeficiency on the level of CD34-positive cells in the cervix
There are many studies on changes in cervix,
however, there are no data about property of cervical tissue to keep
potential for regeneration under influence of immunodeficiency,
which is very important since it allows escaping severe
irreversible changes in tissue. CD34 stem cells are one of
significant indicator for property of tissual regeneration. So, the
purpose of our study was to identify amount of CD34 stem cells
in the cervix under influence of immunodeficiency of infectious
and non-infectious origin.
Materials and methods. Sectional material of reproductive
women was studied. All subjects were divided into 3 groups:
women who were diagnosed with HIV infection; women
who have identified anamnestic and postmortem signs of
alcohol abuse; group of comparison. After routine testing and
immunohistochemical (IHC) staining to CD34, morphometric
measure was performed. We evaluate presence activities of
stromal cells for detection of connection and relationship
between expression of CD34 and the thickness of the cervical
epithelium, relative volumes of condyloma, cervical dysplasia
severity, degree of infiltration of the mucosal lamina propria
by immunocompetent cells. The obtained digital data were
statistically processed.
Results. Morphological investigation revealed changes of
thickness of the cervical stratified squamous nonkeratinized
epithelium up to 714.23Β±59.21 x 10-6 m in group of HIVinfected women. Relative volumes of condylomas were increased in both investigated groups with presence of pointed,
flat and inverted types. Both low- and high-grade squamous
intraepithelial lesions were detected more often in investigated
goups. Assessment of the degree of infiltration of the mucosal
lamina propria by immunocompetent cells was changed
unevenly with reducing in HIV-group and increasing in alcohol
group. Results of ICH reaction CD34 realized in cytoplasmic
staining with membranous accentuation in all cases of control
group with strong, but reduced level in investigated groups.
Most close connection was observed for cervical dysplasia
severity and CD34 expression.
Conclusions.HIV infection and alcohol abuse have pronounced
pathological effects with cervical changes. The expression of
CD34 is present in 96% of women with immunodeficiency
mainly with strong reaction. It is statistically likely that it does
not depend on such morphological indicators as thickness of the
cervical epithelium, relative volumes of condyloma, degree of
infiltration of the mucosal lamina propria by immunocompetent
cells. The expression of CD34 has statistically close negative
connection with cervical dysplasia severity (r=-0,81) and can be
used for detection of early potential of tissual transformation in
women with immunodeficiency
The status of cervical cytology in HIV-infected women
ΠΡΡΠ²Π»Π΅Π½ΠΈΠ΅ ΡΠΈΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΡΠ΅ΡΠ²ΠΈΠΊΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΏΠΈΡΠ΅Π»ΠΈΡ Ρ ΠΠΠ§-ΠΈΠ½ΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΆΠ΅Π½ΡΠΈΠ½ Π² ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠΈ Ρ ΡΡΠΎΠ²Π½Π΅ΠΌ CD4-ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠΎΡΠ»ΡΠΆΠΈΠ»ΠΎ ΡΠ΅Π»ΡΡ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ.
ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΠΈΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· 60 ΠΆΠ΅Π½ΡΠΈΠ½ ΡΠ΅ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π²ΠΎΠ·ΡΠ°ΡΡΠ°, ΠΊΠΎΡΠΎΡΡΠ΅ Π±ΡΠ»ΠΈ
ΡΠ°Π·Π΄Π΅Π»Π΅Π½Ρ Π½Π° 2 Π³ΡΡΠΏΠΏΡ ΠΏΠΎ 30 ΠΆΠ΅Π½ΡΠΈΠ½ (ΠΠΠ§-Π½Π΅Π³Π°ΡΠΈΠ²Π½ΡΠ΅ ΠΈ ΠΠΠ§-ΠΏΠΎΠ·ΠΈΡΠΈΠ²Π½ΡΠ΅). ΠΡΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠΈ
ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² CD4 ΠΌΠ΅Π½ΡΡΠ΅, Π³ΡΠ°Π΄Π°ΡΠΈΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡ Ρ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌΠΈ 500-350-100 ΠΊΠ»Π΅ΡΠΎΠΊ Π² ΠΌΠΈΠΊΡΠΎΠ»ΠΈΡΡΠ΅.
Π Ρ
ΠΎΠ΄Π΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ ΠΎΠ± ΡΡΠΊΠΎΡΠ΅Π½ΠΈΠ΅ Π½Π΅ΠΎΠΏΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ Ρ ΠΠΠ§-
ΠΈΠ½ΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΏΡΠΈ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠΈ ΡΡΠΎΠ²Π½Ρ CD4-ΠΊΠ»Π΅ΡΠΎΠΊ, ΡΡΠΎ ΠΏΡΠΎΡΠ²Π»ΡΠ΅ΡΡΡ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠΌ ΡΠΈΠΏΠΎΠΌ ΠΌΠ°Π·ΠΊΠ°,
ΠΊΠΎΠΉΠ»ΠΎΡΠΈΡΠΎΠ·ΠΎΠΌ, Π½Π°Π»ΠΈΡΠΈΠ΅ΠΌ Π΄Π²ΡΡ
- ΠΈ ΠΌΠ½ΠΎΠ³ΠΎΡΠ΄Π΅ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ, ΠΊΠ΅ΡΠ°ΡΠΈΠ½ΠΎΡΠΈΡΠΎΠ², ΠΏΠ°ΡΠ°ΠΊΠ΅ΡΠ°ΡΠΎΠ·Π°, Π°ΠΌΡΠΎΡΠΈΠ»ΠΈΠΈ
ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΡ, ΠΈ, ΠΊΠ»ΡΡΠ΅Π²ΠΎΠ΅, ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ Π°ΡΡ
ΠΈΡΠ΅ΠΊΡΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅ΡΠ²ΠΈΠΊΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΏΠΈΡΠ΅Π»ΠΈΡ. ΠΠΎ ΠΌΠ½Π΅Π½ΠΈΡ
Π°Π²ΡΠΎΡΠΎΠ² Ρ ΠΠΠ§-ΠΈΠ½ΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΡΠΈΡΠ»ΠΎ CD4-ΠΊΠ»Π΅ΡΠΎΠΊ Π½ΠΈΠΆΠ΅ 100 Π² ΠΌΠΌ3 ΡΠ»Π΅Π΄ΡΠ΅Ρ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡ ΠΊΠ°ΠΊ
Π½Π΅Π±Π»Π°Π³ΠΎΠΏΡΠΈΡΡΠ½ΡΠΉ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠ°ΠΊΡΠΎΡ Π΄Π»Ρ ΡΠ°Π·Π²ΠΈΡΠΈΡ Π½Π΅ΠΎΠΏΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ.The scientific research mentioned in the article was conducted to identify the cytological features of the
cervical epithelium and the level of CD4 cells in HIV-infected women. Cytological analysis was performed
on 60 reproductive-aged women, they were divided into 2 groups equally. 1st group consist of HIV-negative,
2nd group consists of HIV-positive women. When determining low numbers CD4 lymphocytes gradation was
performed with 500-350-100 cells per microliter.
This research works results show the acceleration of neoplastic processes of the cervix in HIV-infected
women with a decreased number of CD4 cells. It is manifested by inflammatory processes in the smear,
koilocytosis, the presence of binuclear and multinucleated cells, keratinocytes, parakeratosis, amphophilic
cytoplasm and changes in cervical epithelial cells.
In HIV-infected people, the CD4 cell count below 100 per cubic millimeter should be considered an
unfavourable prognostic factor for the development of neoplastic transformatio
Morphofunctional state of the fallopian tube in HIV-infected
ΠΠ°Π½Π½Π°Ρ ΡΠ°Π±ΠΎΡΠ° ΠΏΠΎΡΠ²ΡΡΠ΅Π½Π° ΡΠΊΠ°Π½Π΅Π²ΠΎΠΉ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΠΌΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΡΡΠ±Ρ ΠΏΡΠΈ Π½Π°Π»ΠΈΡΠΈΠΈ ΠΠΠ§ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ ΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° Π½Π° ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΠΎΠΌ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π΅ ΠΆΠ΅Π½ΡΠΈΠ½ ΡΠ΅ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π²ΠΎΠ·ΡΠ°ΡΡΠ° Ρ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠ΅ΠΌ ΠΌΠ°ΠΊΡΠΎΡΠΊΠΎΠΏΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΠ·ΠΌΠ΅ΡΠ΅Π½ΠΈΠΉ, Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ Ρ ΠΌΠΎΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ
Π°Π½Π°Π»ΠΈΠ·ΠΎΠΌ, ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ (Hsp70, Hsp90, Bcl-2, BAX, p53).
Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Ρ ΠΠΠ§-ΠΈΠ½ΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΡΠΊΠ°Π½Π΅Π²Π°Ρ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΡ ΠΌΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΡΡΠ±Ρ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΠ΅ΡΡΡ ΡΡΠΊΠΎΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΈΠ½Π²ΠΎΠ»ΡΡΠΈΠ²Π½ΡΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² Ρ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΠΌΠΈ ΡΠΊΠ»Π΅ΡΠΎΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡΠΌΠΈ, Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠ΅ΠΉ Π±Π΅Π»ΠΊΠΎΠ² ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° ΡΠ°ΠΏΠ΅ΡΠΎΠ½ΠΎΠ² ΠΈ Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠ΅ΠΉ Π°ΠΏΠΎΠΏΡΠΎΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ². ΠΠ° ΡΠΎΠ½Π΅ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΡ ΡΠ°Π·ΠΌΠ΅ΡΠΎΠ² ΠΎΡΠ³Π°Π½Π°, ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΡΠΉ
ΠΎΠ±ΡΠ΅ΠΌ ΡΠΎΠ΅Π΄ΠΈΠ½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π² ΠΌΡΡΠ΅ΡΠ½ΠΎΠΌ ΡΠ»ΠΎΠ΅ ΡΠ²Π΅Π»ΠΈΡΠΈΠ²Π°Π΅ΡΡΡ Ρ 5,61Β±1,01% Π΄ΠΎ 21,33Β±2,82%,
Π²ΡΡΠΎΡΠ° ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ»ΠΎΡ ΡΠΌΠ΅Π½ΡΡΠ°Π΅ΡΡΡ Ρ 92,01Β±4,03 Ρ
10-6 ΠΌ Π΄ΠΎ 67,30Β±3,83 Ρ
10-6 ΠΌ.
Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, ΠΌΠΎΠΆΠ½ΠΎ ΠΊΠΎΠ½ΡΡΠ°ΡΠΈΡΠΎΠ²Π°ΡΡ, ΡΡΠΎ Ρ ΠΆΠ΅Π½ΡΠΈΠ½ Ρ ΠΠΠ§-ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠ΅ΠΉ Π² ΠΌΠ°ΡΠΎΡΠ½ΠΎΠΉ
ΡΡΡΠ±Π΅ ΡΠΎΡΠΌΠΈΡΡΡΡΡΡ ΡΠΊΠ°Π½Π΅Π²ΡΠ΅ ΠΏΡΠ΅Π΄ΠΏΠΎΡΡΠ»ΠΊΠΈ Π΄Π»Ρ Π½Π΅ΠΎΠΏΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ.This work is devoted to tissue transformation of the fallopian tube in the presence of
HIV infection and was performed on sectional material of women of reproductive age with macroscopic measurements, histological examination with morphometric analysis, immunohistochemical
studies (Hsp70, Hsp90, Bcl-2, BAX, p53).
As a result of the study, it was found that tissue transformation of the fallopian tube is characterized by acceleration of involutive processes with pronounced sclerotic changes, activation of proteins of the chaperone family and activation of apoptotic processes in HIV-infected patients. The
size of the organ is decreased. Tthe relative volume of connective tissue in the muscle layer increases from 5.61 Β± 1.01% to 21.33 Β± 2.82%, the height of the epithelial layer decreases from 92.01 Β±
4.03 x10-6 m to 67.30 Β± 3.83 x10-6 m.
Thus, it can be stated that in women with HIV infection, tissue prerequisites for neoplastic transformation are formed in the fallopian tube