Nonideal quantum detectors in Bayesian formalism

The Bayesian formalism for a continuous measurement of solid-state qubits is derived for a model which takes into account several factors of the detector nonideality. In particular, we consider additional classical output and backaction noises (with finite correlation), together with quantum-limited output and backaction noises, and take into account possible asymmetry of the detector coupling. The formalism is first derived for a single qubit and then generalized to the measurement of entangled qubits.Comment: 10 page

Spectrum of qubit oscillations from Bloch equations

We have developed a formalism suitable for calculation of the output spectrum of a detector continuously measuring quantum coherent oscillations in a solid-state qubit, starting from microscopic Bloch equations. The results coincide with that obtained using Bayesian and master equation approaches. The previous results are generalized to the cases of arbitrary detector response and finite detector temperature.Comment: 8 page

Targeting an Essential GTPase Obg for the Development of Broad-Spectrum Antibiotics

A promising new drug target for the development of novel broad-spectrum antibiotics is the highly conserved small GTPase Obg (YhbZ, CgtA), a protein essential for the survival of all bacteria including Neisseria gonorrhoeae (GC). GC is the agent of gonorrhea, a prevalent sexually transmitted disease resulting in serious consequences on reproductive and neonatal health. A preventive anti-gonorrhea vaccine does not exist, and options for effective antibiotic treatments are increasingly limited. To address the dire need for alternative antimicrobial strategies, we have designed and optimized a 384-well GTPase assay to identify inhibitors of Obg using as a model Obg protein from GC, ObgGC. The assay was validated with a pilot screen of 40,000 compounds and achieved an average Z’ value of 0.58 ± 0.02, which suggests a robust assay amenable to high-throughput screening. We developed secondary assessments for identified lead compounds that utilize the interaction between ObgGC and fluorescent guanine nucleotide analogs, mant-GTP and mant-GDP, and an ObgGC variant with multiple alterations in the G-domains that prevent nucleotide binding. To evaluate the broad-spectrum potential of ObgGC inhibitors, Obg proteins of Klebsiella pneumoniae and methicillin-resistant Staphylococcus aureus were assessed using the colorimetric and fluorescence-based activity assays. These approaches can be useful in identifying broad-spectrum Obg inhibitors and advancing the therapeutic battle against multidrug resistant bacteria

Functional and Structural Studies on the \u3cem\u3eNeisseria gonorrhoeae\u3c/em\u3e GmhA, the First Enzyme in the \u3cem\u3eglycero-manno\u3c/em\u3e-heptose Biosynthesis Pathways, Demonstrate a Critical Role in Lipooligosaccharide Synthesis and Gonococcal Viability

Sedoheptulose-7-phosphate isomerase, GmhA, is the first enzyme in the biosynthesis of nucleotide-activated-glycero-manno-heptoses and an attractive, yet underexploited, target for development of broad-spectrum antibiotics. We demonstrated that GmhA homologs in Neisseria gonorrhoeae and N. meningitidis (hereafter called GmhAGC and GmhANM, respectively) were interchangeable proteins essential for lipooligosaccharide (LOS) synthesis, and their depletion had adverse effects on neisserial viability. In contrast, the Escherichia coli ortholog failed to complement GmhAGC depletion. Furthermore, we showed that GmhAGC is a cytoplasmic enzyme with induced expression at mid-logarithmic phase, upon iron deprivation and anaerobiosis, and conserved in contemporary gonococcal clinical isolates including the 2016 WHO reference strains. The untagged GmhAGCcrystallized as a tetramer in the closed conformation with four zinc ions in the active site, supporting that this is most likely the catalytically active conformation of the enzyme. Finally, site-directed mutagenesis studies showed that the active site residues E65 and H183 were important for LOS synthesis but not for GmhAGC function in bacterial viability. Our studies bring insights into the importance and mechanism of action of GmhA and may ultimately facilitate targeting the enzyme with small molecule inhibitors

Quantum Zeno stabilization in weak continuous measurement of two qubits

We have studied quantum coherent oscillations of two qubits under continuous measurement by a symmetrically coupled mesoscopic detector. The analysis is based on a Bayesian formalism that is applicable to individual quantum systems. Measurement continuously collapses the two-qubit system to one of the sub-spaces of the Bell basis. For a detector with linear response this corresponds to measurement of the total spin of the qubits. In the other extreme of purely quadratic response the operator \sigma_y^1 \sigma_y^2 + \sigma_z^1 \sigma_z^2 is measured. In both cases, collapse naturally leads to spontaneous entanglement which can be identified by measurement of the power spectrum and/or the average current of the detector. Asymmetry between the two qubits results in evolution between the different measurement subspaces. However, when the qubits are even weakly coupled to the detector, a kind of quantum Zeno effect cancels the gradual evolution and replaces it with rare, abrupt switching events. We obtain the asymptotic switching rates for these events and confirm them with numerical simulations. We show how such switching affects the observable power spectrum on different time scales.Comment: 18 pages, 8 eps figures, reference adde