Участие различных популяций лимфоцитов в формировании защиты от менингококковой инфекции

Immunization of mice by synthetic peptides fragments of meningococcus external membrane fibers caused the expressed protection of animals against infection caused by live virulent meningococcus culture serogroup A, B and C. There are leading part T-lymphocytes in protection of mice from this infection here. The analysis of T-cellular populations by means of a method flowing cytophluorometrics has shown increase CD4+ and CD8+ lymphocytes without change of parity CD4+/CD8+. The increase in number of these lymphocytes occurred in late terms after immunization of mice.Целью настоящего исследования является изучение роли различных популяций лимфоцитов, принимающих участие в формировании иммунитета против менингококковой инфекции при иммунизации синтетическими пептидными фрагментами белков наружной мембраны менингококка серогруппы В. Иммунизация мышей синтетическими пептидными фрагментами белков наружной мембраны менингококка вызывала выраженную защиту животных от заражения живой вирулентной культурой менингококков серогрупп А, В и С. Показана ведущая роль Т-лимфоцитов в защите мышей от этой инфекции. Анализ Т-клеточных популяций с помощью метода проточной цитофлюорометрии показал изменение численности CD4+ и CD8+ лимфоцитов на разных этапах иммуногенеза без изменения соотношения CD4+/CD8+. Увеличение числа этих лимфоцитов происходило в поздние сроки после иммунизации мышей. Данные литературы указывают на роль PorA антигена в формировании Т-клеточного ответа. Тем не менее тип иммунного ответа на бактериальный менингит до сих пор до конца не ясен

Mechanisms of defense formation against meningococcal infection in mice immunized with synthetic peptides

Immunization of mice with synthetic peptide fragments of conservative sites of meningococcal outer membrane proteins led to defense formation against infection with virulent serogroup A and B Meningococci. The role of cellular immunity in the formation of defense against meningococcal infection after immunization with the peptides and the possibility of stimulating lymphocyte population with these peptides were demonstrated. © Springer Science+Business Media, Inc. 2007

Mechanisms of defense formation against meningococcal infection in mice immunized with synthetic peptides

Immunization of mice with synthetic peptide fragments of conservative sites of meningococcal outer membrane proteins led to defense formation against infection with virulent serogroup A and B Meningococci. The role of cellular immunity in the formation of defense against meningococcal infection after immunization with the peptides and the possibility of stimulating lymphocyte population with these peptides were demonstrated. © Springer Science+Business Media, Inc. 2007

Protective properties of synthetic peptides of outer meningococcal membrane

Peptide fragments of conservative sites of PorA, OpaB, and NspA proteins of the outer membrane of serogroup B meningococci were synthesized. These peptides caused a pronounced protective effect in immunized mice infected with virulent homologous and heterologous strains of serogroups B and A meningococci. The protective effect appreciably increased, if the studied peptides were associated in a polycomponent preparation, which can be used in the construction of meningococcal bivalent B+A vaccine. © Springer Science+Business Media, Inc. 2005

Induction of Anti-Meningitis Immunity by Synthetic Peptides: II. Immunoactive Synthetic Fragments of the OpaB Protein from Neisseria meningitidis

Mice of various lines were immunized by 11 synthetic peptides that correspond to the sequences of fragments of the OpaB protein from the outer membrane of Neisseria meningitidis involving the known human T-helper epitopes and all the potential mouse T-helper epitopes calculated for the protein. The mice were immunized with the free peptides without their conjugation with a protein carrier. Most of the peptides were found to induce in mice the production of antipeptide antibodies. Mice protection against the experimental infection by a virulent strain of N. meningitidis of the B serotype was studied, and two peptides were shown to exert the most pronounced protective effect

Protective properties of synthetic peptides of outer meningococcal membrane

Peptide fragments of conservative sites of PorA, OpaB, and NspA proteins of the outer membrane of serogroup B meningococci were synthesized. These peptides caused a pronounced protective effect in immunized mice infected with virulent homologous and heterologous strains of serogroups B and A meningococci. The protective effect appreciably increased, if the studied peptides were associated in a polycomponent preparation, which can be used in the construction of meningococcal bivalent B+A vaccine. © Springer Science+Business Media, Inc. 2005

Induction of antimeningitis immunity by synthetic peptides. II. Immunoactive synthetic fragments of the OpaB protein from Neisseria meningitidis

Mice of various lines were immunized by 11 synthetic peptides that correspond to the sequences of fragments of the OpaB protein from the outer membrane of Neisseria meningitidis involving the known human T-helper epitopes and all the potential mouse T-helper epitopes calculated for the protein. The mice were immunized with the free peptides without their conjugation with a protein carrier. Most of the peptides were found to induce in mice the production of antipeptide antibodies. The mice protection against the experimental infection by a virulent strain of N. meningitidis of the B serotype was studied, and two peptides were shown to exert the most pronounced protective effect

Induction of antimeningitis immunity by the synthetic peptides. I. The immunoactive synthetic fragments of porin a from Neisseria meningitidis

Fourteen peptides corresponding to sequences of all the exposed and some of the transmembrane protein regions of porin A from the outer membrane of Neisseria meningitidis strain B:15:P1.7,16 were synthesized. Mice of various lines were immunized with the free peptides not conjugated with any protein carrier. It was shown that the majority of the peptides possess immunogenic properties. Two peptides were identified binding to antibodies present in the serum of mice after meningitis. Protective properties of a number of the synthesized peptides were studied, and three peptide sequences inducing mice protection from an experimental infection with N. meningitidis were identified

Synthesis and biological properties of polysaccharide-peptide conjugates as potential antigens for a vaccine against meningococci of serogroups A and B

A new approach to the development of a vaccine against meningococci of serogroups A and B was proposed. It involves the synthesis of conjugates of high-molecular capsule polysaccharides of the serogroup A meningococcus (PsA) with earlier synthesized protective fragments of membrane proteins from serogroup B meningococci. The conjugates were synthesized using a method that consists of the generation of aldehyde groups by oxidizing free vicinal hydroxyl groups of PsA and subsequent reaction of these groups with amino groups of the peptide. The reaction proceeds with the intermediate formation of the Schiff base, which is reduced to the stable secondary amine. The main parameters of the reaction were optimized in the synthesis of a PsA conjugate with a model peptide and methods of their characterization were developed. The reproducibility and efficiency of the synthetic procedure were demonstrated by the example of synthesis of PsA conjugates with fragments of protein PorA from the outer membrane of the serogroup B meningococcus. It was shown that, when administered without adjuvant, a conjugate of PsA with a protective peptide, which represents an exposed conserved fragment 306-332 of protein PorA, stimulates the formation of antibodies to the peptide and polysaccharide moieties of the molecule and is also capable of decreasing the degree of bacteremia in animals infected with serogroup A and serogroup B meningococci. The approach can be applied to the development of a complex vaccine for serogroup A and serogroup B meningococci. © 2008 MAIK Nauka

Induction of antimeningitis immunity by the synthetic peptides: I. The immunoactive synthetic fragments of porin A from Neisseria meningitidis

Fourteen peptides corresponding to sequences of all the exposed and some of the transmembrane protein regions of porin A from the outer membrane of Neisseria meningitidis strain B: 15:P1.7,16 were synthesized. Mice of various lines were immunized with the free peptides not conjugated with any protein carrier. It was shown that the majority of the peptides possess immunogenic properties. Two peptides were identified binding to antibodies present in the serum of mice after meningitis. Protective properties of a number of the synthesized peptides were studied, and three peptide sequences inducing mice protection to an experimental infection with N. meningitidis were identified. © 2000 MAIK "Nauka/Interperiodica"