Impact of carbon and nitrogen sources on pectinase production of post-harvest fungi

In the present investigation, emphasis has been given on to study the pectinase enzyme production of post-harvest fungi isolated from mango and papaya fruits, under the influence of carbon and nitrogen sources. It was found that, among carbon sources fructose and sucrose induced pectinase activity, while lactose, CMC and starch inhibited the pectinase activity of test fungi. Nitrate source like sodium nitrate, sodium nitrite and calcium nitrate were found to be stimulated the pectinase activity while, ammonium sources in the form of nitrate, phosphate and sulphate were proved inhibitory for pectinase production of all post-harvest fungi

A Survey of some Medicinal Plants for Fungal Diseases from Osmanabad District of Maharashtra State

The present investigation was carried out in various Talukas of Osmanabad District, India during the years 2008 & 2009. In the present research we have selected commonly found and economically important three medicinal plants viz. Aloe vera (L.) Burm. F, Datura metel L. and Withania somnifera (L.) Dunal from various locations of Osmanabad District for the survey of fungal diseases. Plants were examined In situ for disease symptoms as well as samples being collected for laboratory analyses. Fungi were identified using morphological characteristics, and where necessary with molecular techniques. The survey resulted in a range of fungi identified from the target plants. Common diseases on all the three plants were leaf spots, leaf blight and leaf rust causes harmful effects on medicinal value of the plant parts. Alternaria spp. was attacked on Aloe and Datura leaf while Cercospora withaniae causes leaf spot diseases of Withania. Other fungi observed were Fusarium solani, Aspergillus niger, Penicillium spp., etc. These found to be very harmful for the medicinal uses of the plant parts and may be adversely affect to the body

Cellulase enzyme production of post-harvest fungi under the influence of carbon and nitrogen sources

Present research work embodied the effect of nutritional sources like carbon and nitrogen on cellulase enzyme production of post-harvest fungi. It was found that, among carbon sources fructose and sucrose stimulated cellulase activity, while lactose, CMC and starch inhibited the cellulase activity of test fungi. Nitrate source like sodium nitrate, sodium nitrite and calcium nitrate stimulated the cellulase activity while, ammonium sources in the form of nitrate, phosphate and sulphate were proved inhibitory for cellulase production of all post-harvest fungi

Attributes of Seabuckthorn (Hippophae rhamnoides L.) to Meet Nutritional Requirements in High Altitude.

The diet of humans living in different geographical and climatic regions of the earth varies greatly in both quantity and composition of foods. Evidence is accumulating that indicates that there is a high risk of malnutrition at high altitude because of the usual lack of fresh food and environmental factors. Lack of nutritious diet in the difficult terrain is a potential stressor that elicits oxidative stress. The excretion of minerals from the body is higher in high altitude condition. The altered nutritional requirement can be met to a large extend by regular consumption of locally grown fruits and vegetables. Results of analysis of Seabuckthorn growing in Leh valley of Trans-Himalaya showed the presence of high content of multivitamins including vitamin C (275 mg/100g), vitamin A (432.4 IU/100g), vitamin E (3.54 mg/100g), Riboflavin (1.45 mg/100g), Niacin (68.4 mg/100g), Pantothenic acid (0.85 mcg/100g), vitamin B-6 (1.12 mg/100g), and vitamin B-2 (5.4 mcg/100g). Similarly, mineral elements composition revealed high amount of minerals including potassium (647.2 mg/l), calcium (176.6 mg/l), iron (30.9 mg/l), magnesium (22.5 mg/l), phosphorous (84.2 mg/l), sodium (414.2 mg/l), zinc (1.4 mg/l), copper (0.7 mg/l), manganese (1.06 mg/l) and selenium (0.53 mg/l).Defence Science Journal, 2010, 60(2), pp.226-230, DOI:http://dx.doi.org/10.14429/dsj.60.34

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Phenolic content and antioxidant capacity of various solvent extracts from seabuckthorn (Hippophae rhamnoides L.) fruit pulp, seeds, leaves and stem bark

The outcome of various solvent extraction (water, methanol, acidic 50% methanol, 70% acetone, acidic 50% methanol followed by 70% acetone) on the total phenolic content (TPC) and antioxidant capacity of fruit pulp, seeds, leaves and stem bark of seabuckthorn (Hippophae rhamnoides L.) was investigated. The seabuckthorn extracts possess high phenolic content, 1666–13769 mg GAE/100 g d.w. The mean TPC was found highest in seeds (11148) followed by stem bark (10469), leaves (6330) and pulp (3579 mg GAE/100 g d.w.). In general, the 70% acetone and acidic 50% methanol followed by 70% acetone extracts was found to contain significantly higher TPC than those obtained in other extracting solvents. Antioxidant capacity in terms of IC50 value of pulp (3.39 mg ml−1) was up to 7.8 times higher than those reported for stem bark (0.43 mg ml−1) and up to 2.4 times higher than those found in seeds (1.4 mg ml−1). Further, antioxidant capacity by FRAP assay showed that the stem bark possess maximum antioxidant capacity (16.83) followed by seeds (15.26), leaves (12.73) and pulp (12.61), all as mM FeSO4. Significant correlation was found between TPC and antioxidant capacity by DPPH and FRAP assays

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