4 research outputs found
Characterization of serratia species and qualitative detection of serratia marcescens in raw and pasteurized milk by an analytical method based on polymerase chain reaction
Serratia species are opportunistic pathogenic microorganisms primarily known as nosocomial infectious agents, which can also cause food quality problems. The appearance of the extracellular pigment-producing Serratia marcescens in cow’s milk causes its red discoloration, posing a challenge to the dairy industry and food certification laboratories. The detection of the bacterium by conventional procedures based on microbiological methods is time-consuming and labor-intensive, and in many cases does not lead to satisfactory results due to the competitive inhibitory effect of the accompanying microflora. Following the analysis of the relevant literature, the published endpoint PCR methods and the primers used for the detection of S. marcescens were evaluated in in silico and in vitro assays, and then the procedure was tested on farm milk samples. Using the method, a total of 60 raw and pasteurized milk samples were analyzed, more than half of which (i.e., 32) were identified as S. marcescens positive. The significance of our work is mainly represented by the application of the published test methods in food industry practice. Our results highlight to the importance of detecting this bacterial species
Serratia fajok jellemzĂ©se, valamint Serratia marcescens kvalitatĂv kimutatása nyers Ă©s pasztĹ‘rözött tejbĹ‘l polimeráz láncreakciĂłn alapulĂł vizsgálati mĂłdszerrel
A Serratia fajok elsĹ‘sorban nozokomiális (kĂłrházhigiĂ©nĂ©s fertĹ‘zĂ©s – a Szerk.) fertĹ‘zĹ‘kĂ©nt ismert opportunista patogĂ©n mikroorganizmusok, amelyek Ă©lelmiszerminĹ‘sĂ©gi elváltozásokat is okozhatnak. Az extracelluláris pigment-termelĹ‘ Serratia marcescens tehĂ©ntejben valĂł megjelenĂ©se annak piros elszĂnezĹ‘dĂ©sĂ©t okozza, kihĂvások elĂ© állĂtva a tejipart Ă©s az Ă©lelmiszer-minĹ‘sĂtĹ‘ laboratĂłriumokat. A baktĂ©rium kimutatása hagyományos mikrobiolĂłgiai mĂłdszereken alapulĂł eljárásokkal idĹ‘Ă©s munkaigĂ©nyes, ezen tĂşlmenĹ‘en sok esetben nem is vezet eredmĂ©nyre, a kĂsĂ©rĹ‘ mikroflĂłra kompetitĂv gátlĂł hatása miatt. A vonatkozĂł szakirodalom elemzĂ©sĂ©t követĹ‘en a S. marcescens kimutatása kapcsán publikált vĂ©gpont PCR mĂłdszereket Ă©s alkalmazott primereket in silico Ă©s in vitro vizsgálatban Ă©rtĂ©keltĂĽk, majd az eljárást ĂĽzemi tejmintákon teszteltĂĽk. A mĂłdszer alkalmazásával összesen 60 db nyers, illetve pasztĹ‘rözött tejmintát vizsgáltunk meg, amelyeknek több mint felĂ©t (32 db-ot) azonosĂtottuk S. marcescens pozitĂvkĂ©nt. Munkánk jelentĹ‘sĂ©gĂ©t legfĹ‘kĂ©pp a publikált vizsgálati mĂłdszerek Ă©lelmiszeripari gyakorlatban valĂł alkalmazása adja. EredmĂ©nyeink felhĂvják a figyelmet e baktĂ©riumfaj detektálásának a fontosságára
Serratia fajok jellemzĂ©se, valamint Serratia marcescens kvalitatĂv kimutatása nyers Ă©s pasztĹ‘rözött tejbĹ‘l polimeráz láncreakciĂłn alapulĂł vizsgálati mĂłdszerrel
A Serratia fajok elsĹ‘sorban nozokomiális (kĂłrházhigiĂ©nĂ©s fertĹ‘zĂ©s – a Szerk.) fertĹ‘zĹ‘kĂ©nt ismert opportunista patogĂ©n mikroorganizmusok, amelyek Ă©lelmiszer-minĹ‘sĂ©gi elváltozásokat is okozhatnak. Az extracelluláris pigment-termelĹ‘ Serratia marcescens tehĂ©ntejben valĂł megjelenĂ©se annak piros elszĂnezĹ‘dĂ©sĂ©t okozza, kihĂvások elĂ© állĂtva a tejipart Ă©s az Ă©lelmiszer-minĹ‘sĂtĹ‘ laboratĂłriumokat. A baktĂ©rium kimutatása hagyományos mikrobiolĂłgiai mĂłdszereken alapulĂł eljárásokkal idĹ‘- Ă©s munkaigĂ©nyes, ezen tĂşlmenĹ‘en sok esetben nem is vezet eredmĂ©nyre, a kĂsĂ©rĹ‘ mikroflĂłra kompetitĂv gátlĂł hatása miatt. A vonatkozĂł szakirodalom elemzĂ©sĂ©t követĹ‘en a S. marcescens kimutatása kapcsán publikált vĂ©gpont PCR mĂłdszereket Ă©s alkalmazott primereket in silico Ă©s in vitro vizsgálatban Ă©rtĂ©keltĂĽk, majd az eljárást ĂĽzemi tejmintákon teszteltĂĽk. A mĂłdszer alkalmazásával összesen 60 db nyers, illetve pasztĹ‘rözött tejmintát vizsgáltunk meg, amelyeknek több mint felĂ©t (32 db-ot)azonosĂtottuk S. marcescens pozitĂvkĂ©nt. Munkánk jelentĹ‘sĂ©gĂ©t legfĹ‘kĂ©pp a publikált vizsgálati mĂłdszerek Ă©lelmiszeripari gyakorlatban valĂł alkalmazása adja. EredmĂ©nyeink felhĂvják a figyelmet e baktĂ©riumfaj detektálásának a fontosságára
Characterization of Serratia species and qualitative detection of Serratia marcescens in raw and pasteurized milk by an analytical method based on polymerase chain reaction
Serratia species are opportunistic pathogenic microorganisms primarily known as nosocomial infectious agents, which can also cause food quality problems. The appearance of the extracellular pigment-producing Serratia marcescens in cow’s milk causes its red discoloration, posing a challenge to the dairy industry and food certification laboratories. The detection of the bacterium by conventional procedures based on microbiological methods is time-consuming and labor-intensive, and in many cases does not lead to satisfactory results due to the competitive inhibitory effect of the accompanying microflora. Following the analysis of the relevant literature, the published endpoint PCR methods and the primers used for the detection of S. marcescens were evaluated in in silico and in vitro assays, and then the procedure was tested on farm milk samples. Using the method, a total of 60 raw and pasteurized milk samples were analyzed, more than half of which (i.e., 32) were identified as S. marcescens positive. The significance of our work is mainly represented by the application of the published test methods in food industry practice. Our results highlight to the importance of detecting this bacterial species