Microscopic view of protein bodies and starch granules arrangement under SEM.

<p>(A) <i>O2O2</i> genotype normal line, CML543 (B) <i>o2o2</i>-soft and opaque line, MGUQ-102 (C) <i>o2o2</i>-modified QPM, HKI193-1 (D) <i>o16o16</i> genotype (<i>opaque16</i> line) (E) <i>o2o2/o16o16</i> genotype (double mutant) (Yellow arrow: proteinaceous matrix spreading over the round starch granules).</p

Force (N) required in breaking F₃ seeds.

<p>Force (N) required in breaking F₃ seeds.</p

Segregation pattern of SSRs associated with <i>opaque16</i> and <i>opaque2</i>.

<p>Segregation pattern of SSRs associated with <i>opaque16</i> and <i>opaque2</i>.</p

Light box testing of F₂ seeds derived from crosses.

<p>(A) CML161 × QCL3024 (B) CML193 × QCL3024 (C) CML533 × QCL3024 and (D) CML537 × QCL3024.</p

Marker segregation of <i>o16</i>-linked SSR <i>umc1149</i>.

<p>(A) F₁s of the cross of CML161 × QCL3024 (B) F₂ population derived from F₁s of the cross of CML161 × QCL3024.</p

SDS-PAGE analysis of components of zein proteins in <i>o2o2</i>-soft and opaque line, MGUQ-102 (1 & 2 lane); <i>o2o2/o16o16</i> (3 & 4 lane); <i>o2o2</i>-modified QPM, HKI193-1 (5 & 6 lane); <i>O2O2</i> genotype normal line, CML543 (7 & 8 lane) and different <i>o16o16</i> lines (9–14 lane).

<p>The profiling had been done with two replications for each genotype.</p

Average degree of opaqueness (%) in F₂ seeds.

<p>Average degree of opaqueness (%) in F₂ seeds.</p

Ratio of hard and soft endosperm.

<p>(A) <i>o2o2</i>-soft and opaque line, MGUQ-102 (B)<i>O2O2</i> genotype normal line, CML543 (C)<i>o2o2</i>-modified QPM, HKI193-1 (D) <i>o2o2/o16o16</i> segregant (E-F) <i>O2O2/o16o16</i> segregants.</p

Light box testing of different F₃ families seeds in the cross CML161 × QCL3024.

<p>(A) <i>o2o2</i>/<i>o16o16</i>(B)<i>o2o2</i>/<i>O16O16</i> (C) <i>O2O2</i>/<i>o16o16</i> (D) <i>O2O2</i>/<i>O16O16</i>.</p

Average degree of opaqueness (%) of F₃ seeds.

<p>Average degree of opaqueness (%) of F₃ seeds.</p