Integra® - Dermal Regeneration Template and Split Thickness Skin Grafting – A Therapy Approach to Correct Aplasia Cutis Congenita and Epidermolysis Bullosa in Carmi Syndrome

<p><b>Article full text</b></p><p><br></p><p>The full text of this article can be found here<b>. </b><a href="https://link.springer.com/article/10.1007/s13555-018-0237-2">https://link.springer.com/article/10.1007/s13555-018-0237-2</a></p><p></p><p><br></p><p><b>Provide enhanced content for this article</b></p><p><br></p><p>If you are an author of this publication and would like to provide additional enhanced content for your article then please contact <a href="http://www.medengine.com/Redeem/”mailto:[email protected]”"><b>[email protected]</b></a>.</p><p><br></p><p>The journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are aware that the content has been reviewed to the same level as the articles they are being presented alongside. Moreover, all sponsorship and disclosure information is included to provide complete transparency and adherence to good publication practices. This ensures that however the content is reached the reader has a full understanding of its origin. No fees are charged for hosting additional open access content.</p><p><br></p><p>Other enhanced features include, but are not limited to:</p><p><br></p><p>• Slide decks</p><p>• Videos and animations</p><p>• Audio abstracts</p><p> </p><p>• Audio slides</p> <p> </p> <p> </p

A rare case of pseudohyponatremia in a type IA choledochal cyst due to biliary obstruction

A Type IA choledochal cyst is a cystic or fusiform dilatation of the common bile duct affecting the extrahepatic biliary tree. Here, we present a case report of a 3-year-old girl with a prenatal asymptomatic choledochal cyst that in size, leading to intermittent clay-colored stools and aggravated jaundice. Magnetic resonance cholangiopancreatography revealed a large choledochal cyst of 184 cm3 obstructing the extrahepatic biliary tree and pancreatic duct. Clinical chemistry screening revealed asymptomatic hyponatremia of 124 mmol/l, analysed by an indirect ion selective electron (ISE)- based method. Furthermore, the patient had extreme hypercholesterolemia (1975 mg/dl), and an elevated lipoprotein X level (11.9 g/dl). Serum sodium was confirmed by using two additional independent methods: (i) centrifugal ultrafilter with indirect ISE and (ii) blood gas analyze, In contrast to indirect ISE, both methods indicated normal sodium levels, consistent with pseudohyponatremia. Following Roux-en-Y-Hepatojejunostomy, sequential serum sodium levels measured by indirect ISE gradually normalized, but remained within the normal range by direct ISE method. This case shows that biliary obstruction can lead to falsely elevated cholesterol and lipoprotein X analyses and that alternative methods are needed to avoid unnecessary treatments .</p

Overall Survival.

<p>For Kaplan-Meier survival analysis patients were grouped according to positive and negative PGK1 expression. All patients that died during the follow-up period showed positive PGK1 expression, while none of the PGK1 negative patients died. Overall survival of neuroblastoma patients with a PGK1 negative expression was significantly better than that of PGK1 positive patients (<i>p = 0.003</i>).</p

PGK1 and CXCR4 expression, proliferation and inhibition of CXCR4 of neuroblastoma cell lines.

<p>Kelly (<b>A</b>) and SH-EP Tet-21/N (<b>B</b>) neuroblastoma cells were immunostained for PGK1 and CXCR4 expression (<b>Immunohistochemistry</b>). Both cell lines show a positivity for CXCR4 and react to treatment with 20 µg AMD3100 with an inhibition of proliferation (<b>MTT-assay</b>), although only SH-EP Tet-21/N cells reach a significant level of growth reduction. On examination of PGK1 protein expression levels (<b>Western blot</b>) after 48 h of CXCR4 receptor inhibition, treatment with 20 µg AMD3100 leads to a downregulation of PGK1 protein (45 kDa) in SH-EP Tet-21/N but not in Kelly cells. Tubulin (55 kDa) served as control.</p

Patient characteristics.

<p>Patient characteristics.</p

Metastatic sites.

<p>Metastatic sites.</p

DataSheet_1_DNases improve effectiveness of antibiotic treatment in murine polymicrobial sepsis.pdf

IntroductionNeutrophil extracellular traps (NETs) have various beneficial and detrimental effects in the body. It has been reported that some bacteria may evade the immune system when entangled in NETs. Thus, the aim of the current study was to evaluate the effects of a combined DNase and antibiotic therapy in a murine model of abdominal sepsis.MethodsC57BL/6 mice underwent a cecum-ligation-and-puncture procedure. We used wild-type and knockout mice with the same genetic background (PAD4-KO and DNase1-KO). Mice were treated with (I) antibiotics (Metronidazol/Cefuroxime), (II) DNAse1, or (III) with the combination of both; mock-treated mice served as controls. We employed a streak plate procedure and 16s-RNA analysis to evaluate bacterial translocation and quantified NETs formation by ELISA and immune fluorescence. Western blot and proteomics analysis were used to determine inflammation.ResultsA total of n=73 mice were used. Mice that were genetically unable to produce extended NETs or were treated with DNases displayed superior survival and bacterial clearance and reduced inflammation. DNase1 treatment significantly improved clearance of Gram-negative bacteria and survival rates. Importantly, the combination of DNase1 and antibiotics reduced tissue damage, neutrophil activation, and NETs formation in the affected intestinal tissue.ConclusionThe combination of antibiotics with DNase1 ameliorates abdominal sepsis. Gram-negative bacteria are cleared better when NETs are cleaved by DNase1. Future studies on antibiotic therapy should be combined with anti-NETs therapies.</p

Additional file 1: Figure S1. of Epidemiologic analysis of families with isolated anorectal malformations suggests high prevalence of autosomal dominant inheritance

Pedigrees of 13 families with ARM. Eight parent-offspring families with 16 children and five families with healthy parents with two affected children (PPTX 80 kb

Comparison of scratch wound healing of human primary keratinocytes of various origins and under various culture conditions.

<p>Closed wound area per visual field (mm²) of (A) diabetic versus non-diabetic keratinocytes at 4, 8, 12, 24 and 36 h after wounding using K-SFM medium. n = 4 in duplicates (non-diabetic keratinocytes) and n = 3 in duplicates (diabetic keratinocytes) (B) Keratinocytes from juvenile donors under euglycaemic (6 mM glucose), hyperglycaemic (25 mM glucose) and hyperosmolar (6 mM glucose + 19 mM mannitol) conditions at 12 and 24 h after wounding in K-SFM, Dermalife and Epilife medium. n = 4 in duplicates. (C) Keratinocytes from adult, non-diabetic donors under euglycaemic (6 mM) and hyperglycaemic (25 mM) conditions 4, 8, 12, 24 and 36 h after wounding in K-SFM medium. n = 4 in duplicates. (D) Keratinocytes from juvenile donors under euglycaemic, hyperglycaemic and hyperosmolar conditions at 12 and 24 h after wounding in K-SFM without supplements. n = 4 in duplicates; mean ± SEM; *: statistically significant with p < 0.05.</p

Chemical composition and physical characteristics of the TE used.

<p>Chemical composition and physical characteristics of the TE used.</p