Respiratory virus detection in the upper respiratory tract of asymptomatic, community‑dwelling older people

Background: The prevalence of virus positivity in the upper respiratory tract of asymptomatic community-dwelling older people remains elusive. Our objective was to investigate the prevalence of respiratory virus PCR positivity in asymptomatic community-dwelling older people using saliva samples and nasopharyngeal and oropharyngeal swabs.Methods: We analyzed 504 community-dwelling adults aged ≥ 65 years who were ambulatory and enrolled in a cross-sectional study conducted from February to December 2018 in Nagasaki city, Japan. Fourteen respiratory viruses were identified in saliva, nasopharyngeal and oropharyngeal samples using multiplex PCR assays.Results: The prevalences of PCR positivity for rhinovirus, influenza A, enterovirus and any respiratory virus were 12.9% (95% CI: 10.1–16.1%), 7.1% (95% CI: 5.1–9.8%), 6.9% (95% CI: 4.9–9.5%) and 25.2% (95% CI: 21.5–29.2%), respectively. Rhinovirus was detected in 21.5% of subjects, influenza A in 38.9% of subjects, enterovirus in 51.4% of subjects and any virus in 32.3% of subjects using only saliva sampling.Conclusions: The prevalences of several respiratory viruses were higher than the percentages reported previously in pharyngeal samples from younger adults. Saliva sampling is a potentially useful method for respiratory virus detection in asymptomatic populations

Development of a Highly Sensitive Method for the Detection of Cryptosporidium parvum Virus Type 1 (CSpV1)

Poster Session

Effects of heat-killed Enterococcus faecalis T-110 supplementation on gut immunity, gut flora, and intestinal infection in naturally aged hamsters.

Infectious diseases are a threat to elderly individuals, whose immune systems weaken with age. Among the various infectious diseases, Clostridium difficile infection is associated with a high rate of mortality in elderly individuals and is a serious health problem worldwide, owing to the increasing infection rates. Probiotic use has been proposed as an effective countermeasure for C. difficile infection. The aim of this study was to evaluate the effects of heat-killed Enterococcus faecalis T-110 on intestinal immunity, intestinal flora, and intestinal infections, especially C. difficile infections, in naturally ageing animals, for extrapolating the results to elderly human subjects. Twenty female hamsters were randomly distributed into two groups. Group 1 was fed a basal diet and group 2 was fed a basal diet supplemented with heat-killed E. faecalis for 7 days. Heat-killed E. faecalis T-110 improved the gut immunity and microflora, especially Clostridium perfringens and C. difficile, in naturally aged hamsters. Therefore, heat-killed E. faecalis T-110 use may be a countermeasure against age-related immune dysfunction and intestinal infections, especially C. difficile infection, in elderly humans. However, further investigation in this regard is needed in humans

Effect of dietary probiotics on the semen traits and antioxidative activity of male broiler breeders

Abstract This study aimed to investigate the effect of probiotics on the intestinal morphology, intestinal microflora, oxidative activity (biological antioxidant potential), and semen quality of male broiler breeders. For this, 180 Cobb male broiler breeders (60 weeks of age) were randomly distributed into two groups. The control group was fed a basal diet, and the probiotics group was fed basal diet supplemented with probiotics for 6 weeks. Probiotics containing Bacillus amyloliquefaciens TOA5001 improved the above mentioned characteristics of the male broiler breeders. Thus, B. amyloliquefaciens TOA5001 might improve the reproductive performance of male broiler breeders

Blood biochemical values in Japanese Black breeding cows in Kagoshima Prefecture, Japan

16 pág.Los programas de rehabilitación cardíaca incluyen principalmente trabajo aeróbico, es importante combinar este tipo de actividades con el entrenamiento de fuerza debido a la influencia ejercida sobre ciertas cualidades físicas que van a potencializar los beneficios del ejercicio en pacientes de rehabilitación cardíaca. Objetivo: Evaluar cambios en cualidades físicas de pacientes fase III de rehabilitación cardíaca, posterior a la aplicación de un programa de fuerza. Metodología: Estudio cuasi experimental. Se reclutaron 35 pacientes, mayores de 40 años, test banda igual o mayor de 6METs; se realizó una evaluación inicial de composición corporal, test de fuerza, propiocepción y flexibilidad; 36 sesiones de un programa de fuerza, con una evaluación final de las mismas. El análisis se hizo con medidas de frecuencia, medidas de tendencia de central y dispersión, análisis de Wilcoxon. El trabajo aprobado por el Comité de Ética en Investigación en seres humanos. Resultados: Hombres 19 (54,3%). Edad promedio de 65,2 años. Se observó diferencia significativa p=0,0000 en la mediana del peso levantado, número de repeticiones en la extensión de rodilla, fuerza de agarre y propiocepción después de la intervención. No se observó diferencia en composición corporal y flexibilidad. Conclusión: Se observaron cambio en la fuerza y propiocepción después del entrenamiento de fuerza en pacientes con comorbilidades y mayores de 40 años, en usuarios de un programa de rehabilitación cardíaca. Para ver cambios en flexibilidad es necesario realizar un entrenamiento a más grupos musculares. No se presentaron eventos adversos

Effects of vitamin C supplementation on the blood oxidative stress and antibody titre against Histophilus somni vaccination in calves

The purpose of this study was to determine the effects of vitamin C supplementation on blood oxidative stress biomarkers and antibody response to vaccination in calves