Molecular Characterization of β-1,3-Glucanase Gene in Pathogenic Fungi Tolerant and Susceptible Rubber Tree Clones

ABSTRACTβ-1,3-glucanase is a major lutoidic protein in lactiferous cells of rubber tree. It belongs to a pathogenesis-related (PR) protein family and is classified in PR2 group. It hasbeen reported that the expression of β-1,3-glucanase gene was induced by pathogenic fungi and wounding. In this research, to understand the relation of β-1,3-glucanase gene and pathogenic fungi tolerance in rubber tree, the expression pattern of β-1,3-glucanase gene was analyzed in non-infected plants of pathogenic fungi tolerance rubber tree clone (BPM24) and susceptible one (RRIM600) using quantitative real time polymerase chain reaction (qRT-PCR). In addition, the promoter of β-1,3-glucanase gene obtained from whole genome shotgun contigs in the National Center for Biotechnology Information (NCBI) database of BPM24 and RRIM600 rubber tree clones were analyzed in silico. The results showed that the expression of β-1,3-glucanase gene was significantly higher in BPM24 than RRIM600. Many cis-acting regulatory elements responsive to several hormones, biotic and abiotic stresses were found on β-1,3-glucanasepromoters. Higher number of defense responsive elements was found on the β-1,3-glucanase promoter of BPM24 than RRIM600. The knowledge obtained from this study leads to a betterunderstanding of the expression and regulation of β-1,3-glucanase gene in rubber tree which is beneficial for the improvement of rubber tree clone through pathogenic fungi resistance breeding program.Keywords: β-1,3-glucanase, Pathogenesis-related proteins, Rubber tre

Etude des mécanismes physiologiques de la coagulation du latex d'"Hevea" (identification et caractérisation des protéines impliquées dans l'agrégation des particules de caoutchouc)

MONTPELLIER-BU Sciences (341722106) / SudocSudocFranceF

A Cosmetic Perspective on the Antioxidant Flavonoids from <i>Nymphaea lotus</i> L.

Nymphaea lotus L. or water lily is a well-known traditional medicinal plant in Thailand, Indonesia, Vietnam, India, Sri Lanka, China, Nepal, Egypt and many African countries. This species has been reported as a promising flavonoid-rich raw material that can be used as an active ingredient for the development of cosmetic/cosmeceutical products. This review aims to illustrate the cosmetic potential of this species by providing botanical information, traditional uses, flavonoid accumulation, biological activities and future research challenges in the production of N. lotus extracts for cosmetic applications

A Cosmetic Perspective on the Antioxidant Flavonoids from Nymphaea lotus L.

Nymphaea lotus L. or water lily is a well-known traditional medicinal plant in Thailand, Indonesia, Vietnam, India, Sri Lanka, China, Nepal, Egypt and many African countries. This species has been reported as a promising flavonoid-rich raw material that can be used as an active ingredient for the development of cosmetic/cosmeceutical products. This review aims to illustrate the cosmetic potential of this species by providing botanical information, traditional uses, flavonoid accumulation, biological activities and future research challenges in the production of N. lotus extracts for cosmetic applications

Taxonomic revision of Clinacanthus (Acanthaceae) in Thailand

The taxonomy of Clinacanthus in Thailand is revised. Nomenclature, descriptions and a key to the species are provided. Clinacanthus nutans is lectotypified. Clinacanthus burmanni var. robinsoni from Vietnam is found to be distinct from both C. nutans and C. siamensis and is elevated to species status

Differential expression of mitochondrial pyruvate dehydrogenase gene correlates with latex yield and tapping in rubber tree

Natural rubber (cis-1,4-polyisoprene) is a product of the isoprenoid biosynthesis pathway which requires an allylic pyrophosphate and isopentenyl pyrophosphate (IPP) to initiate and elongate the rubber molecule. The biosynthesis of IPP occurs via two distinct routes: the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways. In this study, the expression of 34 genes related to rubber biosynthesis were compared between high and low latex yielding trees of two rubber tree clones, PB 217 and PB 260. Almost all tested genes revealed no significantly differential expression related to latex yield. Only mitochondrial pyruvate dehydrogenase (PDCE1) showed specific up-regulation in the high latex yielding trees of both tested clones. Interestingly, the expression of PDCE1 involving in the production of acetyl-CoA in mitochondria was also significantly induced by latex loss upon tapping. The increasing of acetyl-CoA and energy production may favor rubber tree to produce more latex. The in silico analysis showed that HbPDCE1 promoter contained ethylene and copper-responsive elements. Ethylene is worldwide used rubber stimulant while copper sulfate was also reported to be able to stimulate the latex yield. This suggested that HbPDCE1 may be transcriptionally regulated by these two compounds however the in vivo regulation of this gene should be further investigated