Auswertung von diallelen Kreuzungsversuchen zur Schaetzung von Kreuzungsparametern ergaenzt durch ein Beispiel aus der Schweinezucht

Es werden 5 Methoden zur Schaetzung von Kreuzungsparametern verglichen. Die Eigenschaften der verschiedenen genetischen Modelle und ihrer statistischen Auswertungsverfahren wurden an einem Zuchtbeispiel geprueft. Als optimales Verfahren wird die Anwendung desEinzeltiermodelles mit vollstaendiger Verwandtschaftmatrix herausgestelltAvailable from: Hohenheim Univ., Stuttgart (Germany, F.R.). Universitaetsbibliothek / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Genipin as a sporicidal agent for the treatment of cortical bone allografts

INTRODUCTION: Microbial bioburden in bone allografts can be reduced by gamma radiation; however, the radiation compromises collagen and increases the risk of graft failure. Genipin is an agent that may reduce bioburden by chemical crosslinking without causing mechanical detriment or cytotoxicity. METHODS: To evaluate genipin\u27s ability to penetrate cortical bone while maintaining sporicidal activity, Bacillus subtilis spore strips were isolated between slices of bovine femoral cortical bone and immersed in genipin solutions for up to one week; spore viability was assessed with media-based assays. The mechanical effects of genipin treatment were assessed by performing three-point bending tests on genipin-treated cortical beams. Cytotoxicity studies were conducted by evaluating the adhesion and proliferation of murine MC3T3-E1 (P21) preosteoblasts on cortical bone slices which were treated with genipin and rinsed to different extents. RESULTS: Genipin successfully penetrated cortical bone slices and sterilized B. subtilis populations after 48 hours (p\u3e0.05) and one week (p CONCLUSIONS: Genipin solutions can sterilize bacterial spore populations entrapped within the continuum of bone tissue while preserving mechanical properties of bone and allowing cell adhesion and proliferation. Provided that antimicrobial effects seen with bacterial spores extend broadly to all microbial and viral species, genipin holds strong potential for bone allograft sterilization