6 research outputs found
Mob as tumor suppressor is activated by Hippo kinase for growth inhibition in Drosophila
Tissue growth and organ size are determined by coordinated cell proliferation and apoptosis in development. Recent studies have demonstrated that Hippo (Hpo) signaling plays a crucial role in coordinating these processes by restricting cell proliferation and promoting apoptosis. Here we provide evidence that the Mob as tumor suppressor protein, Mats, functions as a key component of the Hpo signaling pathway. We found that Mats associates with Hpo in a protein complex and is a target of the Hpo serine/threonine protein kinase. Mats phosphorylation by Hpo increases its affinity with Warts (Wts)/large tumor suppressor (Lats) serine/threonine protein kinase and ability to upregulate Wts catalytic activity to target downstream molecules such as Yorkie (Yki). Consistently, our epistatic analysis suggests that mats acts downstream of hpo. Coexpression analysis indicated that Mats can indeed potentiate Hpo-mediated growth inhibition in vivo. Our results support a model in which Mats is activated by Hpo through phosphorylation for growth inhibition, and this regulatory mechanism is conserved from flies to mammals
CDK-dependent phosphorylation of Mob2 is essential for hyphal development in Candida albicans
In yeast, CDKs and the NDR kinase Cbk1 are regulators of polarized growth. It is found that the CDK Cdc28 regulates the function of Cbk1 in response to hypha-inducing conditions by direct phosphorylation of Mob2, a conserved regulatory subunit of Cbk1