5 research outputs found
Cell-penetrating peptide exploited syndecans
AbstractCell-penetrating peptides (CPPs) are short peptides capable of translocating across the plasma membrane of live cells and transporting conjugated compounds intracellularly. Fifteen years after discovering the first model cationic CPPs, penetratin and TAT, CPP internalization is still challenging many questions. Particularly it has been unknown whether CPPs enter the cells with or without mediation of a specific surface receptor. Here we report that syndecan-4, the universally expressed isoform of the syndecan family of transmembrane proteoglycans, binds and mediates transport of the three most frequently utilized cationic CPPs (penetratin, octaarginine and TAT) into the cells. Quantitative uptake studies and mutational analyses demonstrate that attachment of the cationic CPPs is mediated by specific interactions between the heparan sulfate chains of syndecan-4 and the CPPs. Protein kinase C alpha is also heavily involved in the uptake mechanism. The collected data give the first direct evidence on the receptor-mediated uptake of cationic CPPs and may replace the long-thought, but already contradicted membrane penetration hypothesis. Thus our study might give an answer for a decade long debate and foster the development of rationalized, syndecan-4 targeted novel delivery technologies
Vanillin Analogues o-Vanillin and 2,4,6-Trihydroxybenzaldehyde Inhibit NF kappa B Activation and Suppress Growth of A375 Human Melanoma
Background/Aim: Constitutive activation of nuclear factor kappa-B (NF kappa B) is a hallmark of various cancer types, including melanoma. Chemotherapy may further increase tumour NF kappa B activity, a phenomenon that, in turn, exacerbates drug resistance. This study aimed at preliminary screening of a panel of aromatic aldehydes, including vanillin, for cytotoxicity and suppression of tumour cell NF kappa B activity. Materials and Methods: The cytotoxic and NF kappa B-inhibitory effects of 10 aromatic aldehydes, including vanillin, were investigated in cultured A375 human melanoma cells. Each compound was assayed alone and in combination with the model NF kappa B-activating drug doxorubicin. The most promising analogues were then tested alone and in combination with 4-hydroperoxycyclophosphamide in vitro, and with cyclophosphamide in mice bearing A375 xenografts. Results: The vanillin analogues o-vanillin and 2,4,6-trihydroxybenzaldehyde exhibited cytotoxicity against cultured A375 cells, and inhibited doxorubicin-and 4-hydroperoxycyclophosphamide-induced NF kappa B activation. They also suppressed A375 cell growth in mice. Conclusion: o-vanillin and 2,4,6-trihydroxybenzaldehyde deserve further evaluation as potential anticancer drugs
Contribution of syndecans to lipoplex-mediated gene delivery.
The long awaited breakthrough of gene therapy significantly depends on the in vivo efficiency of targeted intracellular delivery. Hidden details of cellular uptake present a great hurdle for non-viral gene delivery with liposomes. Growing scientific evidence supports the involvement of polyanionic cell surface carbohydrates in cellular internalization of cationic liposomes. Syndecans, a highly conserved family of transmembrane heparan sulfate proteoglycans serve attachment sites for great variety of cationic ligands including growth factors, cytokines and even parasites. In the present study we quantitatively measured the contribution of various syndecan isoforms to liposome-mediated gene transfer. The obtained data show the superiority of syndecan-4, the ubiquitously expressed isoform of the syndecan family, in cellular uptake of liposomes. Applied mutational analysis demonstrated that gene delivery could be abolished by mutating the glycosaminoglycan attachment site of syndecans, highlighting the importance of polyanionic heparan sulfate side chains in the attachment of cationic liposomes. Blocking sulfation of syndecans also diminished gene delivery, a finding that confirms the essential role of polyanionic charges in binding cationic liposomes. Mutating other parts of the syndecan extracellular domain, including the cell-binding domain, had clearly smaller effect on liposome internalization. Mutational analyses also revealed that superiority of syndecan-4 in liposome-mediated gene delivery is significantly influenced by its cytoplasmic domain that orchestrates signaling pathways leading to macropinocytosis. In summary our study present a mechanistic insight into syndecan-mediated macropinocytic uptake of lipoplexes and highlights syndecan-4 as a superior target for cationic liposomes
Köszöntések Dr. Balázs L. György 65. születésnapjára
Rövid összefoglaló az ünnepelt 65. születésnapján elhangzott köszöntő előadásból
Divalent Heavy Metal Cations Block the TRPV1 Ca(2+) Channel
Transient receptor potential vanilloid 1 (TRPV1)
is a non-selective cation channel involved in pain sensation
and in a wide range of non-pain-related physiological and
pathological conditions. The aim of the present study was to
explore the effects of selected heavy metal cations on the
function of TRPV1. The cations ranked in the following
sequence of pore-blocking activity: Co2+ [half-maximal inhibitory
concentration (IC50)013 μM]>Cd2+ (IC500
38 μM)>Ni2+ (IC50062 μM)>Cu2+(IC500200 μM). Zn2+
proved to be a weak (IC50027 μM) and only partial inhibitor
of the channel function, whereas Mg2+, Mn2+ and La3+
did not exhibit any substantial effect. Co2+, the most potent
channel blocker, was able not only to compete with Ca2+ but
also to pass with it through the open channel of TRPV1. In
response to heat activation or vanilloid treatment, Co2+
accumulation was verified in TRPV1-transfected cell lines
and in the TRPV1+ dorsal root ganglion neurons. The
inhibitory effect was also demonstrated in vivo. Co2+ applied
together with vanilloid agonists attenuated the nocifensive
eye wipe response in mice. Different rat TRPV1
pore point mutants (Y627W, N628W, D646N and E651W)
were created that can validate the binding site of previously
used channel blockers in agonist-evoked 45Ca2+ influx
assays in cells expressing TRPV1. The IC50 of Co2+ on
these point mutants were determined to be reasonably comparable
to those on the wild type, which suggests that
divalent cations passing through the TRPV1 channel use
the same negatively charged amino acids as Ca2+