Treatment of Nonalcoholic Fatty Disease in Day Care Municipal Polyclinics

Treatment of patients NFDL in Municipal clinic allows comprehensive treatment of this disease on an autpatient basic, which leads to improved well-being of patients, normal of biocamical parameters

Creating a tobacco line with a weaker antifeedant property against colorado potato beetle

Background. Genetic modification of plants is one of the promising strategies to increase their resistance to insect pests. The development of metabolic or RNA interference systems for plant protection requires appropriate models of host-insect interactions. Nicotiana tabacum L. is a classical model plant used in molecular and metabolic engineering. We consider tobacco as a model for developing protective strategies against Colorado potato beetle (Leptinotarsa decemlineata Say, CPB). Normally, tobacco is toxic for CPB due to high content of nicotine and related alkaloids in leaves. Modification of the tobacco genome could provide tobacco genotypes with altered metabolism suitable for CPB feeding. It is known that different mutations in Berberine Bridge-Like (BBL) genes cause different alterations in tobacco leaf alkaloid levels. In the current study, the Cas9/gRNA system targeting members of the BBL gene family of tobacco was used to create a line which can serve as a diet for CPB. Results. In order to obtain tobacco with modified alkaloid content, two gRNAs matching target sequences in six BBL genes were selected. Each gRNA was cloned into a gRNA/Cas9 generic vector. The created constructs were mixed and used for biolistic transformation of tobacco leaf explants together with the pBI121 plasmid harboring the kanamycin resistance gene nptII and the reporter E.coli betaglucuronidase (GUS) gene. Regenerants were selected on 100 mg/l of kanamycin and checked for transgene presence by histochemical GUS-assay. Unexpectedly, the regenerated plants displayed a variety of adverse phenotypic effects including different degree of growth and rooting inhibition, early flowering, increased number of internodes, changes in leaf shape, fusion of flowers, longostyly, and partial sterility. Only one from seven obtained calli produced a population of regenerated plants without severe phenotypic abnormalities. The NtaBBL5-14 line of clonally propagated plants was selected from this population and used for a CPB feeding experiment. It was shown that CPB larvae consume the leaves of NtaBBL5-14 line ten times more efficiently than the leaves of control plants (97±0.5% vs. 9±3% in 24 h respectively). Conclusion. The NtaBBL5-14 tobacco line is suitable for CPB feeding and can be further used as a model for studies in plant-pest interaction. The modification of other genes regulating nicotine metabolism can be a promising strategy to obtain tobacco plants edible for CPB with less pleiotropic effects

ЗНАЧЕНИЕ ЛАБОРАТОРНОГО МОНИТОРИНГА ОПАСНЫХ ИНФЕКЦИОННЫХ ЗАБОЛЕВАНИЙ В АСПЕКТЕ ПРОТИВОЭПИДЕМИЧЕСКОЙ ЗАЩИТЫ ПРИ ЧРЕЗВЫЧАЙНЫХ СИТУАЦИЯХ БИОЛОГО-СОЦИАЛЬНОГО ХАРАКТЕРА

The most important direction in the system of anti-epidemic protection of the population and military personnel from dangerous infectious and natural focal diseases is epidemiological monitoring with the obligatory use of laboratory methods of detection of pathogens and their markers. On separate territories of placement of the military units which are a part of the southern military district, there are centers of natural and focal infections of dangerous infectious diseases (plague, tularemia, cholera). The article presents the results of laboratory studies during epidemiological monitoring of natural focal and dangerous infectious diseases, which can be used by the military medical service as an information basis for decision-making on the organization of anti-epidemic protection of troops in the event of emergency situations of biological and social nature in these areas.Важнейшим направлением в системе противоэпидемической защиты населения и военнослужащих от опасных инфекционных и природно-очаговых заболеваний является эпидемиологический мониторинг с обязательным использованием лабораторных методов обнаружения возбудителей и их маркеров. На отдельных территориях размещения воинских частей, входящих в состав Южного военного округа, существуют очаги природно-очаговых инфекций опасных инфекционных заболеваний (чума, туляремия, холера), а также природно-очаговых инфекций (клещевой энцефалит). В статье приведены результаты лабораторных исследований в рамках эпидемиологического мониторинга за опасными инфекционными и природно-очаговыми заболеваниями, которые могут быть использованы военно-медицинской службой как информационная основа для принятия решений по организации противоэпидемической защиты войск при возникновении на данных территориях чрезвычайных ситуаций биолого-социального характера

Особенности формирования заболеваемости военнослужащих острыми респираторными инфекциями верхних дыхательных путей

The article sets out the views on the causes of the incidence of various categories of military personnel with acute respiratory infections of the upper respiratory tract (hereinafter – ARI URT), as having the greatest military and epidemiological significance for almost all military contingents.According to the results of a study of literature data, two groups of reasons for the formation of the incidence of acute respiratory infections of the airborne diseases are identified: external (associated with the exposure of military personnel to specific factors of military service) and internal (associated with the characteristics of individual susceptibility to this group of infections).Based on the results of a retrospective epidemiological analysis of the incidence of military servicemen on conscription, the development features of the epidemic process of ARI URT in military units and compounds are shown.According to the results of a retrospective epidemiological analysis of the incidence rate of cadets at the S.M. Kirov Military Medical Academy for 2011-2017 and a comparative analysis of the reversal rate for completed cases, the presence and relatively stable proportion of military personnel exposed to frequent ARI diseases of the airborne diseases throughout the entire 6-year period has been shown period of study.The results of a combined socio-psychological study in groups of often and rarely ill cadets are shown, showing individual signs and psychological characteristics, according to which individual military personnel can be assigned to the risk contingents of increased susceptibility to ARI URT for the purpose of organizing personalized sanitary-antipyretic (preventive) measures.В статье изложены взгляды на причины формирования заболеваемости разных категорий военнослужащих острыми респираторными инфекциями верхних дыхательных путей как имеющими наибольшую военноэпидемиологическую значимость практически для всех воинских контингентов. По результатам изучения литературных данных выделены две группы причин формирования заболеваемости острых респираторных инфекций верхних дыхательных путей: внешние (связанные с воздействием на военнослужащих специфических факторов военной службы) и внутренние (связанные с особенностями индивидуальной восприимчивости к данной группе инфекций). На основе результатов ретроспективного эпидемиологического анализа заболеваемости военнослужащих по призыву показаны особенности развития эпидемического процесса острых респираторных инфекций верхних дыхательных путей в воинских частях и соединениях. По результатам ретроспективного эпидемиологического анализа персонифицированной заболеваемости курсантов Военномедицинской академии им. С.М. Кирова за 2011–2017 гг. и сравнительного анализа обращаемости по законченным случаям показаны наличие и относительно стабильная доля военнослужащих, подверженных частым заболеваниям острых респираторных инфекций верхних дыхательных путей на протяжении всего 6-летнего периода обучения. Приведены результаты комбинированного социопсихологического исследования в группах часто и редко болеющих курсантов, показывающие индивидуальные признаки и психологические особенности, по которым отдельные военнослужащие могут быть отнесены к контингентам риска повышенной восприимчивости к острым респираторным инфекциям верхних дыхательных путей для целей организации персонализированных санитарно-противоэпидемических (профилактических) мероприятий

Chronic kidney disease in patients with psoriasis, chronic opisthorchiasis and at their combination

The aim of the investigation was to study of features of сhronic kidney disease in patients with chronic opisthorchiasis (CO), psoriasis (Ps) and their combination. Examination was performed on 360 patients with CO, 100 patients with Ps, 50 patients with Ps combinated CO and 30 healthy subjects. In addition to complete clinical and instrumental examination accepted in specialised clinic, biopsy of kidney was conducted. The interrelation of intensity of sclerous processes in kidneys and renoprival syndrome with duration of CO and activity of Ps was found. Dehelmintization with biltricid promoted improvement of functional state of kidneys

Seedling Biometry of <i>nud</i> Knockout and <i>win1</i> Knockout Barley Lines under Ionizing Radiation

The genes NUD and WIN1 play a regulatory role in cuticle organization in barley. A knockout (KO) of each gene may alter plant mechanisms of adaptation to adverse environmental conditions. A putative pleiotropic effect of NUD or WIN1 gene mutations in barley can be assessed in a series of experiments in the presence or absence of a provoking factor. Ionizing radiation is widely used in research as a provoking factor for quantifying adaptive potential of living organisms. Our aim was to evaluate initial stages of growth and development of barley lines with a KO of NUD or WIN1 under radiation stress. Air-dried barley grains with different KOs and wild-type control (WT) were exposed to γ-radiation at 50, 100, or 200 Gy at a dose rate of 0.74 R/min. Approximately 30 physiological parameters were evaluated, combined into groups: (1) viability, (2) radiosensitivity, and (3) mutability of barley seed progeny. Seed germination, seedling survival, and shoot length were similar among all barley lines. Naked nud KO lines showed lower weights of seeds, roots, and seedlings and shorter root length as compared to win1 KO lines. The shoot-to-root length ratio of nud KO lines’ seedlings exceeded that of win1 KO and WT lines. In terms of the number of seedlings with leaves, all the KO lines were more sensitive to pre-sowing γ-irradiation. Meanwhile, the radioresistance of nud KO lines (50% growth reduction dose [RD50] = 318–356 Gy) and WT plants (RD50 = 414 Gy) judging by seedling weight was higher than that of win1 KO lines (RD50 = 201–300 Gy). Resistance of nud KO lines to radiation was also demonstrated by means of root length (RD50 = 202–254 Gy) and the shoot-to-root length ratio. WT seedlings had the fewest morphological anomalies. In nud KO lines, mainly alterations of root shape were found, whereas in win1 KO lines, changes in the color and shape of leaves were noted. Thus, seedlings of nud KO lines are characterized mainly by changes in the root system (root length, root number, and root anomalies). For win1 KO lines, other parameters are sensitive (shoot length and alterations of leaf shape and color). These data may indicate a pleiotropic effect of genes NUD and WIN1 in barley

The value of laboratory monitoring of dangerous infectious diseases in the aspect of anti-epidemic protection in emergency situations of biological character

The most important direction in the system of anti-epidemic protection of the population and military personnel from dangerous infectious and natural focal diseases is epidemiological monitoring with the obligatory use of laboratory methods of detection of pathogens and their markers. On separate territories of placement of the military units which are a part of the southern military district, there are centers of natural and focal infections of dangerous infectious diseases (plague, tularemia, cholera). The article presents the results of laboratory studies during epidemiological monitoring of natural focal and dangerous infectious diseases, which can be used by the military medical service as an information basis for decision-making on the organization of anti-epidemic protection of troops in the event of emergency situations of biological and social nature in these areas

Features of formation of the military personnel’s morbidity of acute respiratory infections of the upper respiratory tract

The article sets out the views on the causes of the incidence of various categories of military personnel with acute respiratory infections of the upper respiratory tract (hereinafter – ARI URT), as having the greatest military and epidemiological significance for almost all military contingents.According to the results of a study of literature data, two groups of reasons for the formation of the incidence of acute respiratory infections of the airborne diseases are identified: external (associated with the exposure of military personnel to specific factors of military service) and internal (associated with the characteristics of individual susceptibility to this group of infections).Based on the results of a retrospective epidemiological analysis of the incidence of military servicemen on conscription, the development features of the epidemic process of ARI URT in military units and compounds are shown.According to the results of a retrospective epidemiological analysis of the incidence rate of cadets at the S.M. Kirov Military Medical Academy for 2011-2017 and a comparative analysis of the reversal rate for completed cases, the presence and relatively stable proportion of military personnel exposed to frequent ARI diseases of the airborne diseases throughout the entire 6-year period has been shown period of study.The results of a combined socio-psychological study in groups of often and rarely ill cadets are shown, showing individual signs and psychological characteristics, according to which individual military personnel can be assigned to the risk contingents of increased susceptibility to ARI URT for the purpose of organizing personalized sanitary-antipyretic (preventive) measures