IT Competence in Internet Founder Teams - An Analysis of Preferences and Product Innovativity

In the Net Economy, numerous start-ups relying on Internet-based business models have been founded in the recent years. In these ventures IT experts are confronted with different requirements to those of traditional software development. It can thus be assumed that founders in the Net Economy prefer IT experts with a different competence profile. Based on an elaborate competence model for IT experts in Internet-based ventures, founder preferences are empirically analyzed and related to the novelty of the venture’s product. An adaptive conjoint analysis is applied to obtain utility values for single components of competence. Using cluster analysis, four different competence profiles are identified which correspond to prototypical IT experts bearing different core functions. Data analysis suggests that founders with more innovative products differ from founders with less innovative products in their perception of the optimal IT expert’s competence profile. The results have implications both for career decisions of IT experts and for founders of Internet start-ups who are looking for co-founding IT experts. This study is one of the first to explicitly focus on IT competence in Internet-based ventures. It therefore extends existing research on IT competence to a new and dynamic industry

Age-Related Gene Expression Differences in Monocytes from Human Neonates, Young Adults, and Older Adults.

A variety of age-related differences in the innate and adaptive immune systems have been proposed to contribute to the increased susceptibility to infection of human neonates and older adults. The emergence of RNA sequencing (RNA-seq) provides an opportunity to obtain an unbiased, comprehensive, and quantitative view of gene expression differences in defined cell types from different age groups. An examination of ex vivo human monocyte responses to lipopolysaccharide stimulation or Listeria monocytogenes infection by RNA-seq revealed extensive similarities between neonates, young adults, and older adults, with an unexpectedly small number of genes exhibiting statistically significant age-dependent differences. By examining the differentially induced genes in the context of transcription factor binding motifs and RNA-seq data sets from mutant mouse strains, a previously described deficiency in interferon response factor-3 activity could be implicated in most of the differences between newborns and young adults. Contrary to these observations, older adults exhibited elevated expression of inflammatory genes at baseline, yet the responses following stimulation correlated more closely with those observed in younger adults. Notably, major differences in the expression of constitutively expressed genes were not observed, suggesting that the age-related differences are driven by environmental influences rather than cell-autonomous differences in monocyte development

Flow cytometry data standards

Background: Flow cytometry is a widely used analytical technique for examining microscopic particles, such as cells. The Flow Cytometry Standard (FCS) was developed in 1984 for storing flow data and it is supported by all instrument and third party software vendors. However, FCS does not capture the full scope of flow cytometry (FCM)-related data and metadata, and data standards have recently been developed to address this shortcoming. Findings. The Data Standards Task Force (DSTF) of the International Society for the Advancement of Cytometry (ISAC) has developed several data standards to complement the raw data encoded in FCS files. Efforts started with the Minimum Information about a Flow Cytometry Experiment, a minimal data reporting standard of details necessary to include when publishing FCM experiments to facilitate third party understanding. MIFlowCyt is now being recommended to authors by publishers as part of manuscript submission, and manuscripts are being checked by reviewers and editors for compliance. Gating-ML was then introduced to capture gating descriptions - an essential part of FCM data analysis describing the selection of cell populations of interest. The Classification Results File Format was developed to accommodate results of the gating process, mostly within the context of automated clustering. Additionally, the Archival Cytometry Standard bundles data with all the other components describing experiments. Here, we introduce these recent standards and provide the very first example of how they can be used to report FCM data including analysis and results in a standardized, computationally exchangeable form. Conclusions: Reporting standards and open file formats are essential for scientific collaboration and independent validation. The recently developed FCM data standards are now being incorporated into third party software tools and data repositories, which will ultimately facilitate understanding and data reuse. © 2011 Brinkman et al; licensee BioMed Central Ltd

Personality composition and performance in entrepreneurial teams:understanding the impact of stability and plasticity traits in a relative contribution model

Considering that personalities of entrepreneurs are diverse, we examine how the personality composition of entrepreneurial teams affects team performance. Given the specific challenges of the entrepreneurship setting, we suggest a new understanding of the team composition-team performance link by (1) taking a meta-perspective to personality that considers the stability vs. plasticity underlying the Big Five traits and by (2) applying a relative contribution (minimum/maximum) conceptualisation of team composition. We conduct a quantitative study with 104 entrepreneurial teams. Our findings indicate that high entrepreneurial team performance requires all team members to have minimum levels of stability-related traits (agreeableness, emotional stability, conscientiousness), but only one team member with high plasticity-related traits (openness, extraversion)

Energy Demands of Early Life Drive a Disease Tolerant Phenotype and Dictate Outcome in Neonatal Bacterial Sepsis

Bacterial sepsis is one of the leading causes of death in newborns. In the face of growing antibiotic resistance, it is crucial to understand the pathology behind the disease in order to develop effective interventions. Neonatal susceptibility to sepsis can no longer be attributed to simple immune immaturity in the face of mounting evidence that the neonatal immune system is tightly regulated and well controlled. The neonatal immune response is consistent with a “disease tolerance” defense strategy (minimizing harm from immunopathology) whereas adults tend toward a “disease resistance” strategy (minimizing harm from pathogens). One major advantage of disease tolerance is that is less energetically demanding than disease resistance, consistent with the energetic limitations of early life. Immune effector cells enacting disease resistance responses switch to aerobic glycolysis upon TLR stimulation and require steady glycolytic flux to maintain the inflammatory phenotype. Rapid and intense upregulation of glucose uptake by immune cells necessitates an increased reliance on fatty acid metabolism to (a) fuel vital tissue function and (b) produce immunoregulatory intermediates which help control the magnitude of inflammation. Increasing disease resistance requires more energy: while adults have fat and protein stores to catabolize, neonates must reallocate resources away from critical growth and development. This understanding of sepsis pathology helps to explain many of the differences between neonatal and adult immune responses. Taking into account the central role of metabolism in the host response to infection and the severe metabolic demands of early life, it emerges that the striking clinical susceptibility to bacterial infection of the newborn is at its core a problem of metabolism. The evidence supporting this novel hypothesis, which has profound implications for interventions, is presented in this review

Eras of Digital Entrepreneurship

While recent research continues to emphasize the importance of digital entrepreneurship, the historical terminology of this field is often overlooked. Digital entrepreneurship tends to be considered a new phenomenon despite emerging in the early 1990s. Building on a scoping literature review, this study analyzes 1354 publications that use nine different terms interchangeably to describe the phenomenon of digital entrepreneurship. Based on the number of publications per year, three eras in the historical development of digital entrepreneurship research are outlined. Digital technologies are identified as external enablers, and certain practical events are considered to be influencing factors. The results show that recent research has not adequately recognized the contributions of previous publications and that the understanding of digital entrepreneurship is quite similar with regard to the terms used and over time. This study shows how emerging digital technologies, such as artificial intelligence, blockchain technology, and big data analytics, might shape the future of digital entrepreneurship research. The study occupies the intersection between entrepreneurship and information systems literature and its main contribution is to provide new insights into the eras of digital entrepreneurship from the past to the present and into the future

Correlation analysis of intracellular and secreted cytokines via the generalized integrated mean fluorescence intensity

The immune response in humans is usually assessed using immunogenicity assays to provide biomarkers as correlates of protection (CoP). Flow cytometry is the assay of choice to measure intracellular cytokine staining (ICS) of cell-mediated immune (CMI) biomarkers. For CMI analysis, the integrated mean fluorescence intensity (iMFI) was introduced as a metric to represent the total functional CMI response as a CoP. iMFI is computed by multiplying the relative frequency (percent positive) of cells expressing a particular cytokine with the MFI of that population, and correlates better with protection in challenge models than either the percentage or the MFI of the cytokine-positive population. While determination of the iMFI as a CoP can readily be accomplished in animal models that allow challenge/protection experiments, this is not feasible in humans for ethical reasons. As a first step toward extending the iMFI concept to humans, we investigated the correlation of the iMFI derived from a human innate immune response ICS assay with functional cytokine release into the culture supeRNAtant, as innate cytokines need to be released to have a functional impact. Next, we developed a quantitatively more correlative mathematical approach for calculating the functional response of cytokine-producing cells by incorporating the assignment of different weights to the magnitude (frequency of cytokine-positive cells) and the quality (the MFI) of the observed innate immune response. We refer to this model as generalized iMFI. © 2010 Interantional Society for Advancement of Cytometry