15 research outputs found
Dysregulated placental microRNAs in Early and Late onset Preeclampsia
Copyright © 2017. Published by Elsevier Ltd.INTRODUCTION: To determine the miRNA expression profile in placentas complicated by Preeclampsia (PE) and compare it to uncomplicated pregnancies. METHODS: Sixteen placentas from women with PE, [11 with early onset PE (EOPE) and 5 with late onset PE (LOPE)], as well as 8 placentas from uncomplicated pregnancies were analyzed using miRNA microarrays. For statistical analyses the MATLAB® simulation environment was applied. The over-expression of miR-518a-5p was verified using Quantitative Real-Time Polymerase Chain Reaction. RESULTS: Forty four miRNAs were found dysregulated in PE complicated placentas. Statistical analysis revealed that miR-431, miR-518a-5p and miR-124* were over-expressed in EOPE complicated placentas as compared to controls, whereas miR-544 and miR-3942 were down-regulated in EOPE. When comparing the miRNA expression profile in cases with PE and PE-growth restricted fetuses (FGR), miR-431 and miR-518a-5p were found over-expressed in pregnancies complicated by FGR. DISCUSSION: Since specific miRNAs can differentiate EOPE and LOPE from uncomplicated placentas, they may be considered as putative PE-specific biomarkers. MiR-518a-5p emerged as a potential diagnostic indicator for EOPE cases as well as for PE-FGR complicated placentas, indicating a potential link to the severity of the disease.Peer reviewe
Urine proteomic studies in preeclampsia
Preeclampsia (PE) is a multisystem disorder of pregnancy that develops
after 20 wk of gestation in previously normotensive women and
complicates 5-8% of pregnancies. This rapidly progressive syndrome is
usually diagnosed when the mother develops hypertension and proteinuria.
The only effective treatment is delivery of the baby although early
low-dose aspirin has been shown to significantly reduce the risk for PE.
Recent advances in proteomic methods of protein separation,
identification, and quantitation may allow for the identification of
proteins and peptides that could facilitate early detection of disease,
improve assessment of prognosis, and allow closer monitoring of women at
risk for PE. This review summarizes all currently available markers for
prediction and diagnosis of PE and presents urine proteomic studies
performed for the identification of novel biomarkers
First Trimester Maternal Plasma Aberrant miRNA Expression Associated with Spontaneous Preterm Birth
Spontaneous Preterm Delivery (sPTD) is one of the leading causes of perinatal mortality and morbidity worldwide. The present case–control study aims to detect miRNAs differentially expressed in the first trimester maternal plasma with the view to identify predictive biomarkers for sPTD, between 320/7 and 366/7 weeks, that will allow for timely interventions for this serious pregnancy complication. Small RNA sequencing (small RNA-seq) of five samples from women with a subsequent sPTD and their matched controls revealed significant down-regulation of miR-23b-5p and miR-125a-3p in sPTD cases compared to controls, whereas miR-4732-5p was significantly overexpressed. Results were confirmed by qRT-PCR in an independent cohort of 29 sPTD cases and 29 controls. Statistical analysis demonstrated that miR-125a is a promising early predictor for sPTL (AUC: 0.895; 95% CI: 0.814-0.972; p < 0.001), independent of the confounding factors tested, providing a useful basis for the development of a novel non-invasive predictive test to assist clinicians in estimating patient-specific risk
Proteomic analysis of amniotic fluid in pregnancies with Down syndrome
Proteomic analysis is widely used for the detection of diagnostic markers. In the present study amniotic fluid supernatants?(AFS) from pregnancies with Down syndrome?(DS) fetuses and from chromosomally normal fetuses in the 17th?week of gestation were analyzed by 2-DE. Gel comparison revealed significant differences in the two groups. Spots with different expression levels were excised and proteins were identified by MALDI-MS and nano-ESI-MS/MS. Splicing factor arginine/serine-rich?4 (SFRS4; Q08170) was present only in AFS from DS fetuses and completely absent in the control group. Quantitative differences were detected for alpha-1-microglobulin (AMBP; P02760), collagen alpha?1?(I) chain (CO1A1; P02452), collagen alpha?1?(III) chain (CO3A1; P02461), collagen alpha?1?(V) chain?d (CO5A1; P20908), and basement membrane-specific heparin sulfate proteoglycan core protein (PGBM; P98160). These proteins were increased in cases with DS, whereas protein?IBP-1 (P08833) was decreased by 40% compared with chromosomally normal fetuses. Four proteins, CO1A1, CO3A1, CO5A1, and PGBM, appeared as fragments. As differentially expressed proteins were present in all pregnancies with DS tested, they may represent useful potential markers for prenatal diagnosis. However, for protein biomarkers to be of any clinical utility, systematic analysis of the maternal serum should be conducted
Proteomic analysis of amniotic fluid in pregnancies with Down syndrome
Proteomic analysis is widely used for the detection of diagnostic
markers. in the present study amniotic fluid supernatants (AFS) from
pregnancies with Down syndrome (DS) fetuses and from chromosomally
normal fetuses in the 17th week of gestation were analyzed by 2-DE. Gel
comparison revealed significant differences in the two groups. Spots
with different expression levels were excised and proteins were
identified by MALDI-MS and nano-ESI-MS/MS. Splicing factor
arginine/serine-rich 4 (SFRS4; Q08170) was present only in AFS from DS
fetuses and completely absent in the control group. Quantitative
differences were detected for alpha-1-microglobulin (AMBP; P02760),
collagen alpha 1 (1) chain (CO1A1; P02452), collagen alpha 1 (III) chain
(CO3A1; P02461), collagen alpha 1 (V) chain d (CO5A1; P20908), and
basement membrane-specific heparin sulfate proteoglycan core protein
(PGBM; P98160). These proteins were increased in cases with DS, whereas
protein IBP-1 (P08833) was decreased by 40% compared with chromosomally
normal fetuses. Four proteins, CO1A1, CO3A1, CO5A1, and PGBM, appeared
as fragments. As differentially expressed proteins were present in all
pregnancies with DS tested, they may represent useful potential markers
for prenatal diagnosis. However, for protein biomarkers to be of any
clinical utility, systematic analysis of the maternal serum should be
conducted
Proteomic analysis of amniotic fluid in pregnancies with Klinefelter syndrome foetuses
Klinefelter syndrome is a sex chromosomal abnormality (47, XXY
karyotype), occuring approximately in 1 in 1000 male live births In the
present study proteomic analysis was performed in twelve 2nd trimester
amniotic fluid samples, eight coming from pregnancies with normal males
and four with Klinefelter syndrome foetuses, as shown by routine
prenatal cytogenetic analysis Samples were analysed by 2-DE, coupled
with MALDI-TOF-MS analysis. Three proteins (Ceruloplasmin,
Alpha-1-antitrypsin and Zinc-alpha-2-glycoprotein) were found to be
up-regulated in samples obtained from pregnancies with Klinefelter
syndrome foetuses, whereas four proteins (Apolipoprotein A-I, Plasma
retinal-binding protein, Gelsolin, and Vitamin D-binding protein) were
down regulated when compared to proteins detected in samples from normal
foetuses The differential expression of Ceruloplasmin, Apolipoprotein
A-I and Plasma retinol-binding protein was further confirmed by
immunoblotting. Since these proteins are likely to cross the placenta
barrier and be detected in maternal plasma they could be used as
biomarkers for the non-invasive prenatal diagnosis of Klinefelter
syndrome (C) 2009 Elsevier B V All rights reserve