Attachment accuracy of a novel prototype robotic rotary and investigation of two management strategies for incomplete milked quarters

Throughout 2009 and 2010, FutureDairy (Camden, NSW, Australia) was involved in testing a novel prototype robotic rotary (RR). The commercial version RR is expected to be capable of carrying out 90 milkings per hour. To achieve the high throughput the rotary rotates the cow to the cup attachment robot and then around the platform in a stop–start fashion. The robot does not remain with the cow during the entire milking process. When not all teat cups are attached during a milking session there is an opportunity for cows to be sent back to the waiting yard for a second milking attempt. The study presented here was designed to test whether or not the extension of the interval to a second milking attempt improved milking success of incompletely milked cows. It was expected that with an increased milking interval between the two subsequent milkings the changes to the udder conformation could positively affect the attachment success at the second attempt. The 1 h milking interval treatment (1 h) simulated cows being drafted directly back to the pre-milking waiting yard, whilst the 3 h milking interval treatment (3 h) was designed to simulate cows being drafted back after accessing post-milking supplementary feed on a feedpad. The results presented in this manuscript showed no significant difference between the frequencies of successful attachment in the second attempt between the 1 h and 3 h treatments indicating that a reasonable level of flexibility exists with management of incompletely milked cows and dairy layout designs. Milk production level affected the probability of success at second attempt, which was about 7.5 times higher in cows with an average milk production level greater than 19.3 kg than those with less than 10.8 kg. When looking at the total proportion of cows successfully milked after two attempts, it was found that successful milking was more likely in multiparous cows compared to primiparous cows. Highlights • We studied management options for incompletely milked cows on a robotic rotary. • Firstly cows returned to waiting yard with approx. 1 h interval between attempts. • Secondly cows returned to waiting yard via feed pad – approx. 3 h between attempts. • No difference found between 1 and 3 h intervals on attachment success. • Level of flexibility exists with management of incompletely milked cows. Keywords: Success-rate; Pasture based; Automatic milking system; Robotic rotary; DairyDairy Australia, DeLaval, Department of Primary Industries NSW and The University of Sydney

German evidence-based guidelines for the treatment of Psoriasis vulgaris (short version)

Psoriasis vulgaris is a common and chronic inflammatory skin disease which has the potential to significantly reduce the quality of life in severely affected patients. The incidence of psoriasis in Western industrialized countries ranges from 1.5 to 2%. Despite the large variety of treatment options available, patient surveys have revealed insufficient satisfaction with the efficacy of available treatments and a high rate of medication non-compliance. To optimize the treatment of psoriasis in Germany, the Deutsche Dermatologische Gesellschaft and the Berufsverband Deutscher Dermatologen (BVDD) have initiated a project to develop evidence-based guidelines for the management of psoriasis. The guidelines focus on induction therapy in cases of mild, moderate, and severe plaque-type psoriasis in adults. The short version of the guidelines reported here consist of a series of therapeutic recommendations that are based on a systematic literature search and subsequent discussion with experts in the field; they have been approved by a team of dermatology experts. In addition to the therapeutic recommendations provided in this short version, the full version of the guidelines includes information on contraindications, adverse events, drug interactions, practicality, and costs as well as detailed information on how best to apply the treatments described (for full version, please see Nast et al., JDDG, Suppl 2:S1–S126, 2006; or http://www.psoriasis-leitlinie.de)

Increased expression of CD80, CD86 and CD70 on T cells from HIV-infected individuals upon activation in vitro: regulation by CD4+ T cells

T cells express CD28 and CD27 which transduce co-stimulatory signals after interaction with their ligands on antigen-presenting cells (APC). These ligands, CD80, CD86 and CD70, are also expressed to some extent on activated T cells. Here, we show that in human immunodeficiency virus (HIV)-infected individuals, CD28 and CD27 expression is decreased on CD8+ T cells. On the other hand, T cell stimulation in vitro induced high CD80, CD86 and CD70 expression on T cells from HIV-infected individuals. It appeared that an inverted CD4:CD8 T cell ratio could explain this enhanced expression of co-stimulatory ligands. Indeed, high expression levels of CD80, CD86 and CD70 were found on activated CD8+ T cells from HIV- individuals cultured in the absence of CD4+ T cells. Addition of CD4+ T cells prevented this up-regulation. However, in HIV-infected individuals, addition of excess autologous or healthy control CD4+ T cells did not completely counteract up-regulation of co-stimulatory ligand expression on CD8+ T cells. Thus, to some extent, CD8+ T cells in HIV-infected individuals appeared to be refractory to CD4+ T cell-mediated regulation of ligand expression in vitro. Activated T cells from HIV-infected individuals and activated CD8+ T cells from healthy controls were able to act as accessory cells in CD3-induced T cell proliferation, which was dependent on cell-cell contact. Thus, we showed that T cells from HIV-infected individuals express enhanced levels of co-stimulatory ligands upon activation, which provides them with accessory cell properties. Enhanced stimulatory potential of these nonprofessional APC may contribute to persistently high levels of immune activation in HIV infection related to disease progressio