33 research outputs found
Production of tropane alkaloids in Hyoscyamus niger (black henbane) hairy roots grown in bubble-column and spray bioreactors
Hairy root cultures of Hyoscyamus niger were cultivated in shake-flasks, a bubble-column bioreactor and a hybrid bubble-column/spray bioreactor and evaluated for alkaloid production. The latter gave the highest anisodamine content (0.67 mg/g dry wt) whereas scopolamine, hyoscyamine and cuscohygrine concentrations were highest in the bubble-column reactor (5.3, 1.6 and 26.5 mg/g dry wt, respectively). Both bioreactors gave similar productivities of scopolamine (1 and 0.98 mg/l day) and cuscohygrine (5 and 5.4 mg/l day), but anisodamine productivity was 3.5-fold higher in the hybrid bioreactor (HB) (0.02 and 0.07 mg/l day, respectively). Elicitation with methyl jasmonate increased scopolamine productivity by 146 % in roots grown in the HB whereas their permeabilization with DMSO caused 4-, 5-, 25- and 28-fold increase in scopolamine, hyoscyamine, anisodamine and cuscohygrine concentrations in the growth medium. In situ extraction with Amberlite XAD-2 doubled scopolamine productivity in the hybrid reactor after 50 days
Accumulation of dibenzocyclooctadiene lignans in agar cultures and in stationary and agitated liquid cultures of Schisandra chinensis (Turcz.) Baill
Schisandra chinensis plant in vitro cultures were maintained on Murashige and Skoog (MS) medium supplemented with 3 mg/l 6-benzyladenine (BA) and 1 mg/l 1-naphthaleneacetic acid (NAA) in an agar system and also in two different liquid systems: stationary and agitated. Liquid cultures were grown in batch (30 and 60 days) and fed-batch modes. In the methanolic extracts from lyophilized biomasses and in the media, quantification of fourteen dibenzocyclooctadiene lignans identified based on co-chromatography with authentic standards using high-performance liquid chromatography with diode array detection (HPLC-DAD) and/or liquid chromatography with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI-MS) methods. For comparison purposes, phytochemical analyses were performed of lignans in the leaves and fruits of the parent plant. The main lignans detected in the biomass extracts from all the tested systems were schisandrin (max. 65.62 mg/100 g dry weight (DW)), angeloyl-/tigloylgomisin Q (max. 49.73 mg/100 g DW), deoxyschisandrin (max. 43.65 mg/100 g DW), and gomisin A (max. 34.36 mg/100 g DW). The highest total amounts of lignans in the two tested stationary systems were found in extracts from the biomass harvested after 30 days of batch cultivation: 237.86 mg/100 g DW and 274.65 mg/100 g DW, respectively. In the agitated culture, the total content reached a maximum value of 244.80 mg/100 g DW after 60 days of the fed-batch mode of cultivation. The lignans were not detected in the media. This is the first report which documents the potential usefulness of S. chinensis shoot cultures cultivated in liquid systems for practical purposes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00253-015-7230-9) contains supplementary material, which is available to authorized users
Bioreactor type affects the accumulation of phenolic acids and flavonoids in microshoot cultures of Schisandra chinensis (Turcz.) Baill
Microshoots of the East Asian medicinal plant species Schisandra chinensis (Chinese magnolia vine) were grown in bioreactors
characterized by different construction and cultivation mode. The tested systems included two continuous immersion
systems-a cone-type bioreactor (CNB) and a cylindric tube bioreactor (CTB), a nutrient sprinkle bioreactor (NSB), and two
temporary immersion systems (TIS)-RITA® and Plantform. Microshoots were grown for 30 and 60 days in the MS medium
enriched with 1 mg NAA and 3 mg BA. The accumulation of two groups of phenolic compounds: phenolic acids and
flavonoids in the bioreactor-grown S. chinensis biomass, was evaluated for the first time. In the microshoot extracts, seven
phenolic acids: chlorogenic, gallic, p-hydroxybenzoic, protocatechuic, syringic, salicylic and vanillic, and three flavonoids:
kaempferol, quercitrin and rutoside, were identified. The highest total amount of phenolic acids (46.68 mg 100 DW)
was recorded in the biomass maintained in the CNB for 30 days. The highest total content of flavonoids (29.02 mg 100
DW) was found in the microshoots maintained in the NSB for 30 days. The predominant metabolites in all the tested systems
were: gallic acid (up to 10.01 mg 100 DW), protocatechuic acid (maximal concentration 16.30 mg 100 DW), and
quercitrin (highest content 21.00 mg 100 DW)
Schisandra henryi a species with high medicinal potential and pharmacological activities of dibenzocyclooctadiene lignans
Schisandra henryi C.B. Clarke (family Schisandraceae), is a species endemic to China Yunnan Province. S. henryi is a little known plant in Europe and the United States. Few studies on the chemical composition of S. henryi, mainly by scientists from Chinese institutions, showed the presence of compounds belonging to lignans, triterpenoids and nortriprenoids.
Recently, research by the team of the Department of Pharmaceutical Botany of the Jagiellonian University Medical College has highlighted the great potential of this species. Phytochemical, biological and biotechnological researches are conducted. The analysis of the chemical composition showed the presence of compounds from the group of dibenzylcyclooctadiene, aryltetraline and dibenzylbutane lignans as well as polyphenols - phenolic acids and flavonoids. An innovative aspect of the scientific work is in particular biotechnological research focusing on proposing biomass from in vitro cultures of S. henryi as a source for obtaining extracts and biologically active compounds, alternative to the hard-to-reach material collected from natural sites.
The aim of the study was to review the scientific literature on research on S. henryi, with particular emphasis on its botanical, ecological and chemical characteristics, and biological properties of extracts and isolated compounds. In addition, the potential of biotechnological research conducted on in vitro cultures of S. henryi focusing on understanding their metabolic profile was signaled.
The paper also presents a detailed characterization of dibenzocyclotadiene lignans specific for the Schisandraceae family. They are the subject of numerous scientific studies that confirm their valuable pharmacological properties. Particularly noteworthy are their hepatoprotective and hepatoregenerating properties, as well as: anti-inflammatory, neuroprotective, anticancer, antiviral, antioxidant, cardioprotective, antiosteoporotic and supporting the treatment of intestinal dysfunction