The Effect Infrared and Ultraviolet Radiation in the Development of the Cells in the Porous Permeable Titanium Nickel Based Alloy Scaffold

The respond of the cells to electromagnetic radiation depends on many factors, including microenvironment. Different cells exhibit different behavior when exposed to low-intensity radiation employed in the research. The effect of infrared (IR) and ultraviolet (UV) radiation leads to the reliable change in the number of viable cells. Viable cells of Ehrlich tumor, spleen and bone marrow of C57BL/6 mice placed in a porous permeable TiNi-based alloy scaffold were studied. The level of the radiation effect was determined for different types of cells. A comparative analysis of the viable cells cultured in the porous permeable TiNi scaffold was performed

Evaluation of Porous TiNi-based Alloy as a Scaffold for Liver Tissue Engineering

Complex analysis of development of cells of liver – hepatocytes in the biocompatible scaffold, fabricated of porous-permeable TiNi-based alloy has been performed. Specific stages of development of cellular population in pores of scaffold are displayed in the present investigation. The stepwise characteristic of developing of hepatocytes in porous-permeable structure from TiNi-based alloy is demonstrated. Nanoporous surface of pores in incubator permit cells to be reliably fixed and produce growth to cellular colonies, and porous-permeable structure of scaffold allows penetrating to nutrients from environment during all time of investigation in vivo. Examination on CCl4-induced hepatitis has displayed effective antitoxic activity of population of hepatocytes in scaffold from TiNi-based alloy and their prolonged effect

Study of Correlation of Structural Features in the Porous TiNi-based Materials Obtained by Sintering with the Integration Process of Bone Marrow Cell Populations

The structural features of porous-permeable TiNi-based materials obtained by sintering in depending on the temperature have been studied. It is shown that a material obtained at the sintering temperature of T2=1250 °C and time t=40 min has an optimal degree of sintering and relates to finely porous materials with the porosity of 55 %. A positive dynamics of development of bone marrow cells in the TiNi-based sintered material was proved. It is noted that the finely porous macrostructure with developed rough surface of pore walls provides favorable conditions for development of cell populations. When the 21 day of cultivation a dense formed tissue on basis of bone marrow cells observes in the material pores

Features of Cells Integration on TiNi-based Porous Scaffold

Developing of standard population of cells — line fibroblasts 3T3 in porous structure of incubator having fixed composition, porousness and pore size distribution has been studied. The volume of scaffold space was shown to determine the potential cell division and its function. The characteristic integration of fibroblasts in porous incubators from TiNi-based alloy having different volumes was analyzed. The relation of colonization by cells and growth of tissues in porous samples from titanium nickel depending on depth of penetration is determined