Two-group Differences in Methylation Levels amongAD, aMCI and NC Groups.

<p>Methylation levels obtained using HumanMethylation450 BeadChip were compared between NC, aMCI or AD group (n = 4) and one other group. The numbers of loci having significant differences are indicated.</p

Comparisons between individual background characteristics and <i>COASY</i> promoter region DNA methylation levels.

<p>Comparisons between individual background characteristics and <i>COASY</i> promoter region DNA methylation levels.</p

Comparison of Methylation Levels in NC, aMCI and AD groups.

<p>For <i>COASY</i> gene promoter region: DNA methylation levels in the 3 groups by MS-HRM (A), Correlation between measurement results from MS-HRM and Illumina Infinium HD Methylation Assay (B), receiver operating characteristic (ROC) curve for diagnosis of aMCI and AD (C). For SPINT1 gene promoter region: DNA methylation levels in the 3 groups by MS-HRM (D), Correlation between measurement results from MS-HRM and Illumina Infinium HD Methylation Assay (E), receiver operating characteristic (ROC) curve for diagnosis of aMCI and AD (F). Red horizontal lines are means, error bars indicate S.E.M. ****<i>P</i> < 0.0001; one-way ANOVA with Scheffe’s test.</p

Comparisons between individual background characteristics and <i>SPINT1</i> promoter region DNA methylation levels.

<p>Comparisons between individual background characteristics and <i>SPINT1</i> promoter region DNA methylation levels.</p

Subject characteristics (mean ± S.E.M.)

<p>Subject characteristics (mean ± S.E.M.)</p

Part of Sequences of <i>COASY</i> and <i>SPINT1</i> Promoter Regions.

<p>The open box area indicates the source sequence of Target id: cg01756799 in the <i>COASY</i> promoter region (A) and that of Target id: cg09898695 in the <i>SPINT1</i> (B) promoter region. The gray areas indicate the loci of primers.</p

Part of Sequence <i>NCAPH2</i>/<i>LMF2</i> Promoter Region from Pyrosequencing.

<p>The gray area indicates the source sequence of the Target id: cg25152348. The open box indicates the target sequence of pyrosequencing.</p