2 research outputs found
Kinetic Switching of the Concentration/Separation Behavior of Microdroplets
This
work demonstrates that the solute concentration inside 100
micrometer-sized aqueous microdroplets can be controlled by adjusting
the time required for the aqueous nanometer-sized droplets (nanodroplet)
or reverse micelles to pass over the surface of the microdroplet.
The kinetics of molecular transport between the microdroplets and
the nanodroplets was investigated by utilizing a microdroplet array,
and on the basis of these results, a control over the concentration
selectivity of the contents of the microdroplet was achieved. This
method is operationally simple and can be potentially applied as a
pretreatment method for microanalytical systems that require high-density
microdroplet arrays. This method can also be utilized for parallel
small sample analyses such as single cell analysis
Distribution and Adsorption of Ionic Species into a Liposome Membrane and Their Dependence upon the Species and Concentration of a Coexisting Counterion
The
distribution of ions into a bilayer lipid membrane (BLM) and
their adsorption on the BLM are investigated by extracting a hydrophobic
cation, rhodamine 6G (R6G<sup>+</sup>), into a liposome through the
dialysis membrane method. R6G<sup>+</sup> distribution mainly depends
upon the concentration of the coexisting anion and its species (Cl<sup>–</sup>, Br<sup>–</sup>, BF<sub>4</sub><sup>–</sup>, ClO<sub>4</sub><sup>–</sup>, and picrate). On the other
hand, R6G<sup>+</sup> adsorption on the BLM surface follows the Langmuir
adsorption model and is independent of the coexisting anion in the
aqueous phase. We propose an extraction model of ionic species into
the BLM, to explain the dependence of extraction of ionic species
upon the coexisting anion. In this model, an ion is distributed with
a coexisting counterion into the BLM and then forms an ion pair in
the BLM. Here, the ion adsorption equilibrium on the BLM surface is
independent of the species and concentration of the coexisting counterion
under the same ionic strength. On the basis of this model, we estimate
the distribution constant of R6G<sup>+</sup> and anion (<i>K</i><sub>D</sub>), the ion-pair formation constant in the BLM (<i>K</i><sub>ip</sub>), and the R6G<sup>+</sup> adsorption constant
on the BLM surface (<i>K</i><sub>ad</sub>). Even for an
ultrathin membrane system, such as a BLM, R6G<sup>+</sup> is distributed
with a coexisting counterion and the distribution equilibrium of the
ionic species at the water–BLM interface is analyzable similar
to that at the water–organic solvent interface