29 research outputs found
On inconsistency of experimental data on primary nuclei spectra with sea level muon intensity measurements
For the first time a complete set of the most recent direct data on primary
cosmic ray spectra is used as input into calculations of muon flux at sea level
in wide energy range GeV. Computations have been performed
with the CORSIKA/QGSJET and CORSIKA/VENUS codes. The comparison of the obtained
muon intensity with the data of muon experiments shows, that measurements of
primary nuclei spectra conform to sea level muon data only up to several tens
of GeV and result in essential deficit of muons at higher energies. As it
follows from our examination, uncertainties in muon flux measurements and in
the description of nuclear cascades development are not suitable to explain
this contradiction, and the only remaining factor, leading to this situation,
is underestimation of primary light nuclei fluxes. We have considered
systematic effects, that may distort the results of the primary cosmic ray
measurements with the application of the emulsion chambers. We suggest, that
re-examination of these measurements is required with the employment of
different hadronic interaction models. Also, in our point of view, it is
necessary to perform estimates of possible influence of the fact, that sizable
fraction of events, identified as protons, actually are antiprotons. Study of
these cosmic ray component begins to attract much attention, but today nothing
definite is known for the energies GeV. In any case, to realize whether
the mentioned, or some other reasons are the sources of disagreement of the
data on primaries with the data on muons, the indicated effects should be
thoroughly analyzed
HEX expression and localization in normal mammary gland and breast carcinoma
BACKGROUND: The homeobox gene HEX is expressed in several cell types during different phases of animal development. It encodes for a protein localized in both the nucleus and the cytoplasm. During early mouse development, HEX is expressed in the primitive endoderm of blastocyst. Later, HEX is expressed in developing thyroid, liver, lung, as well as in haematopoietic progenitors and endothelial cells. Absence of nuclear expression has been observed during neoplastic transformation of the thyroid follicular cells. Aim of the present study was to evaluate the localization and the function of the protein HEX in normal and tumoral breast tissues and in breast cancer cell lines. METHODS: HEX expression and nuclear localization were investigated by immunohistochemistry in normal and cancerous breast tissue, as well as in breast cancer cell lines. HEX mRNA levels were evaluated by real-time PCR. Effects of HEX expression on Sodium Iodide Symporter (NIS) gene promoter activity was investigated by HeLa cell transfection. RESULTS: In normal breast HEX was detected both in the nucleus and in the cytoplasm. In both ductal and lobular breast carcinomas, a great reduction of nuclear HEX was observed. In several cells from normal breast tissue as well as in MCF-7 and T47D cell line, HEX was observed in the nucleolus. MCF-7 treatment with all-trans retinoic acid enhanced HEX expression and induced a diffuse nuclear localization. Enhanced HEX expression and diffuse nuclear localization were also obtained when MCF-7 cells were treated with inhibitors of histone deacetylases such as sodium butyrate and trichostatin A. With respect to normal non-lactating breast, the amount of nuclear HEX was greatly increased in lactating tissue. Transfection experiments demonstrated that HEX is able to up-regulate the activity of NIS promoter. CONCLUSION: Our data indicate that localization of HEX is regulated in epithelial breast cells. Since modification of localization occurs during lactation and tumorigenesis, we suggest that HEX may play a role in differentiation of the epithelial breast cell
Synthesis of Highly Substituted Adamantanones from Bicyclo[3.3.1]nonanes
Trifluoromethanesulfonic acid and other electrophiles promote formation of the adamantanone core from the readily accessible 1,5-dimethyl-3,7-dimethylenebicyclo[3.3.1]nonan-9-one 2. Because adamantyl cation 3 can be trapped by a range of nucleophiles, including aromatic and heteroaromatic rings, alcohol, nitriles, and halides, access to a wide variety of functionality at the newly formed tertiary position is provided