Supplementary Material for: The Ameliorating Effect of Lactobacillus gasseri OLL2716 on Functional Dyspepsia in Helicobacter pylori-Uninfected Individuals: A Randomized Controlled Study

<p><b><i>Background/Aims:</i></b> Probiotics appear to improve <i>Helicobacter pylori</i>-associated dyspepsia via an inhibitory effect on <i>H. pylori;</i> however, uncertainty exists regarding their effects in <i>H. pylori</i>-uninfected individuals. We evaluated the efficacy of <i>Lactobacillus gasseri</i> OLL2716 (<i>L. gasseri</i> OLL2716) on <i>H. pylori</i>-uninfected individuals with functional dyspepsia (FD). <b><i>Methods:</i></b> A double-blind, parallel-group, placebo-controlled, randomized, controlled trial was performed. Participants were randomly assigned to ingest <i>L. gasseri</i> OLL2716-containing yogurt (<i>L. gasseri</i> OLL2716 group) or <i>L. gasseri</i> OLL2716-free yogurt (placebo group) for 12 weeks. Participants completed questionnaires that dealt with a global assessment as well as symptom severity. The per-protocol (PP) population was evaluated for efficacy in accordance with a plan prepared beforehand. <b><i>Results:</i></b> Randomization was performed on 116 individuals; the PP population consisted of 106 individuals (mean age 42.8 ± 9.0). The impressions regarding the overall effect on gastric symptoms were more positive in the <i>L. gasseri</i> OLL2716 group compared to that in the placebo group (statistical trend; <i>p</i> = 0.073). The elimination rate for major FD symptoms was 17.3 and 35.3% in the placebo and <i>L. gasseri</i> OLL2716 groups respectively (<i>p</i> = 0.048). <b><i>Conclusion:</i></b><i>L. gasseri</i> OLL2716 has beneficial effects on FD without <i>H. pylori</i> involvement.</p

Supplementary Material for: A case of cystic retroperitoneal dedifferentiated liposarcoma diagnosed by percutaneous image-guided biopsy

Dedifferentiated liposarcoma was initially defined as a tumor containing differentiated liposarcoma and distinct regions of nonlipogenic spindle cell or pleomorphic sarcoma. Retroperitoneal liposarcomas feature a characteristic appearance with a predominantly fatty component, and cystic liposarcomas are rare. We describe a case of retroperitoneal dedifferentiated liposarcoma predominantly consisting of multilocular cysts. A 77-year-old man previously visited a doctor because an echo scan unexpectedly revealed an abdominal tumor. Contrast computed tomography (CT) disclosed a large multilocular cystic tumor spanning from the left upper abdomen to the retroperitoneum, and poorly marginated soft tissue structures were present around the abdominal aorta, inferior vena cava, pancreas, mesentery, and left kidney. CT also revealed a right lung mass. The soft tissue structures in the retroperitoneal cystic tumor and right lung mass were strongly enhanced on 2-deoxy-2-[fluorine-18] fluoro-D-glucose positron emission tomography, suggesting a malignant retroperitoneal tumor and lung metastasis. CT-guided percutaneous biopsy targeting the left perirenal soft tissue structure was performed, and the tumor was diagnosed as dedifferentiated liposarcoma. Lung metastasis was present, and the retroperitoneal tumor surrounded multiple organs. Therefore, the tumor was not suitable for surgical resection, but it was indicated for chemotherapy based on multidisciplinary discussion. We experienced a case of retroperitoneal cystic de-differentiated liposarcoma diagnosed by percutaneous image-guided biopsy and treated appropriately based on the pathological diagnosis