9 research outputs found
Green synthesis of novel biocomposites from treated cellulosic fibers and recycled bio-plastic polylactic acid
This study investigated mechanical properties of biocomposites developed from recycled polylactic acid
(PLA) from packaging industry and treated cellulosic fibers from pulp and paper solid waste. Microwave
and enzymatic treatments were used for extraction and surface modification of hydrophilic cellulosic
fibers. Enzymatic treatment was specifically performed for activation of hydroxyl groups and improvement
of adhesion between matrix and fibers including controlling the length of cellulosic fibers with size
reduction of around 50% (142 and 127 mm for primary and mixed biosolids, respectively) as compared to
microwave treatment. Microwave treatment produced cellulosic fibers of 293 and 341 mm, for primary
and mixed biosolids, respectively. Mechanical properties of biocomposites with 2% (w/w) of treated
cellulosic fibers (Young's Modulus 887.83 MPa with tensile strain at breakpoint of 7.22%, tensile stress at
yield 41.35 MPa) was enhanced in comparison to the recycled PLA (Young's Modulus
644.47 ± 30.086 MPa with tensile strain at breakpoint of 6.01 ± 0.83%, tensile stress at yield of
29.49 ± 3.64 MPa). Scanning electron microscopy revealed size reduction of cellulosic fibers. X-ray
diffraction and Fourier transform infrared spectroscopy confirmed strong mechanical properties of novel
biocomposites.The authors are sincerely thankful to the Natural Sciences and
Engineering Research Council of Canada (Discovery Grant 355254
and NSERC CRD Grant), and CRIBIQ for financial support. We would
like to thank Mr. R. Fortin and Colin Jacob Vaillancourt from Gaudreau
Environment for providing rPLA samples. Likewise, the
support of Ozymes Inc. is equally appreciated for valuable comments
during the experimental planning from industrial perspective.
Financial assistance by the âFonds de recherche du Quebec-
Nature et technologies (FRQNT)â and INRS-ETE has been thankfully
acknowledged by K Hegde.info:eu-repo/semantics/publishedVersio
Biochemische und molekularbiologische Charakterisierung von Alkoholdehydrogenasen und einer Oxygenase aus Rhodococcus Spezies
New feruloyl esterases to access phenolic acids from grass biomass
In the Sorangium cellulosum strain So ce56 genome, two putative esterase-encoding genes (loci sce1896 and sce8927) were cloned, expressed in Escherichia coli, and the resulting enzymes (designated ScFAE1 and ScFAE2) were used to assess the possible release of ferulic acid (FA) from triticale and wheat brans, and an aqueous fraction of steam-exploded wheat straw. The two polypeptides, sharing only 30% sequence identity, exhibit a typical catalytic Ser-Asp-His triad, a characteristic of \u3b1/\u3b2-hydrolase fold proteins. Both ScFAE1 (35 kDa) and ScFAE2 (34 kDa) were purified to apparent homogeneity and comparison of their kinetic parameters indicated an apparent higher affinity of ScFAE2 than ScFAE1 towards the various feruloyl substrates. This property was reflected by the observation that ScFAE2 was capable of yielding up to 85% of FA from destarched triticale bran. In the steam-exploded wheat sample, more than 85% yield of FA or p-coumaric acid was also effected by ScFAE2 without the decomposition of valuable chemical such as furfural. The two cloned FAEs represent the first of myxobacterial origin to be characterized and they are classified as new members of the type D family of FAEs.Peer reviewed: YesNRC publication: Ye
Structural analysis of Bacillus pumilus phenolic acid decarboxylase, a lipocalin-fold enzyme
Acta Crystallographica Section F: Structural Biology and Crystallization Communicationsis a rapid all-electronic journal, which provides a home for short communications onthe crystallization and structure of biological macromolecules. It includes four categoriesof publication: protein structure communications; nucleic acid structure communications;structural genomics communications; and crystallization communications. Structuresdetermined through structural genomics initiatives or from iterative studies suchas those used in the pharmaceutical industry are particularly welcomed. Section F isessential for all those interested in structural biology including molecular biologists, biochemists,crystallization specialists, structural biologists, biophysicists, pharmacologistsand other life scientists.Acta Crystallographica Section F: Structural Biology and Crystallization Communications est une revue enti\ue8rement \ue9lectronique \ue0 parution rapide renfermant de courtes communications sur la cristallisation et la structure de macromol\ue9cules biologiques. Elle se divise en quatre cat\ue9gories de communications, soit la structure des prot\ue9ines, la structure des acides nucl\ue9iques, la g\ue9nomique structurale et la cristallisation. Les structures d\ue9termin\ue9es par des initiatives en g\ue9nomique structurale ou \ue0 partir d\u2019\ue9tudes it\ue9ratives, comme celles employ\ue9es dans l\u2019industrie pharmaceutique, sont particuli\ue8rement bien accueillies. Cette revue constitue un incontournable pour tous ceux et celles qui s\u2019int\ue9ressent \ue0 la biologie structurale, soit les biologistes mol\ue9culaires, les biochimistes, les sp\ue9cialistes de la cristallisation, les biologistes structuraux, les biophysiciens, les pharmacologues et autres chercheurs en sciences de la vie.Peer reviewed: YesNRC publication: Ye
Thermostable feruloyl esterase for the bioproduction of ferulic acid from triticale bran
A putative \u3b1/\u3b2 hydrolase fold-encoding gene (locus tag TTE1809) from the genome of Thermoanaerobacter tengcongensis was cloned and expressed in Escherichia coli as a possible source of thermostable feruloyl esterase (FAE) for the production of antioxidant phenolic acids from biomass. Designated as TtFAE, the 33-kDa protein was purified to apparent homogeneity. The lipase-like sequence characteristics of TtFAE and its substrate specificity towards methyl ferulate, methyl sinapate, and methyl p-coumarate classify it as a new member of the type A FAEs. At 75\ub0C, the enzyme retained at least 95% of its original activity for over 80 min; at 80\ub0C, its half-life was found to be 50 min, rendering TtFAE a highly thermostable protein. Under different hydrolytic conditions, ferulic acid (FA) was shown to be released from feruloylated oligosaccharides prepared from triticale bran. An estimated recovery of 68 mg FA/100 g triticale bran was demonstrated by a 30% release of the total FA from triticale bran within a 5-h incubation period. Both the oxygen radical absorbing capacity values of the feruloylated oligosaccharides and free FA were also determined. Overall, this work introduces a new bacterial member to the growing family of plant cell wall degrading FAEs that at present is largely of fungal origin, and it benchmarks the bioproduction of FA from triticale bran.Peer reviewed: YesNRC publication: Ye
Practical asymmetric enzymatic reduction through discovery of a dehydrogenase-compatible biphasic reaction media
Gröger H, Hummel W, Buchholz S, et al. Practical asymmetric enzymatic reduction through discovery of a dehydrogenase-compatible biphasic reaction media. Organic Letters. 2003;5(2):173-176.[reaction: see text] An enzyme-compatible biphasic reaction media for the asymmetric biocatalytic reduction of ketones with in situ cofactor regeneration has been developed. In this biphasic reaction media, which is advantageous for reactions at higher substrate concentrations, both enzymes (alcohol dehydrogenase and FDH from Candida boidinii) remain stable. The reductions with poorly water-soluble ketones were carried out at substrate concentrations of 10-200 mM, and the optically active (S)-alcohols were formed with moderate to good conversions and with up to >99% ee