Anthocyanini of petunia controls pigment synthesis, vacuolar pH, and seed coat development by genetically distinct mechanisms

ANTHOCYANIN1 (AN1) of petunia is a transcription factor of the basic helix-loop-helix (bHLH) family that is required for the synthesis of anthocyanin pigments. Here, we show that AN1 controls additional aspects of cell differentiation: the acidification of vacuoles in petal cells, and the size and morphology of cells in the seed coat epidermis. We identified an1 alleles, formerly known as ph6, that sustain anthocyanin synthesis but not vacuolar acidification and seed coat morphogenesis. These alleles express truncated proteins lacking the C-terminal half of AN1, including the bHLH domain, at an ∼30-fold higher level than wild-type AN1. An allelic series in which one, two, or three amino acids were inserted into the bHLH domain indicated that this domain is required for both anthocyanin synthesis and vacuolar acidification. These findings show that AN1 controls more aspects of epidermal cell differentiation than previously thought through partially separable domains

Evolution and development of virtual inflorescences.

The architecture of inflorescences diverged during the evolution of distinct plant families by mechanisms that remain unknown. Using computer modeling, Przemyslaw Prusinkiewicz and colleagues established a single model for the development of distinct inflorescences. Selection restricts inflorescence evolution to high fitness paths that vary with climate and other factors that influence reproductive success - explaining why some evolutionary transitions are more likely than others. This model presents an important framework for future plant 'evo-devo' research. © 2007 Elsevier Ltd. All rights reserved

How genes paint flowers.

Mutant analyses have given insight into the various parameters that contribute to flower colour and pattern, which is so important for pollination. One important factor is the accumulation of orange, red and purple anthocyanin pigments in the cell vacuole patterns arise by cell-specific expression of combinations of regulatory proteins. The overall colour perceived is also influenced by vacuolar pH, co-pigmentation and the shape of the petal cells. Although understanding of the biochemistry and genetics of anthocyanin and flavonol biosynthesis is well developed, this is not the case for pH and cellshape control

Transgenes and protein localization: myths and legends

Fluorescent protein (FP) fusions are frequently used to localize and follow the movement of proteins in living cells. However, a consensus is missing about the experimental design and controls that guarantee the reliability of the results. Here, we discuss possible artifacts and try to navigate through the many methods, preferences, and assumptions that surround protein localization in plants that make it difficult to design a universal approach to achieve reliable results. © 2013 Elsevier Ltd