Sample information.

<p>Sample information.</p

PCoA plot representing the distances between samples, expressed by the Bray-Curtis metric.

<p>Each point represents a different sample, while the colored circles, triangles, and squares represent BU, non-BU, and Healthy, respectively.</p

Phylum taxonomy level classifications.

<p>Bar plot showing the relative proportions of the phyla within all the samples.</p

Viability of <i>M. ulcerans</i> (ITM 030216, ITM 980912, ITM 5114, and ITM 842) during infection of <i>A. polyphaga</i> at MOI 1.

<p>Viability of <i>M. ulcerans</i> (ITM 030216, ITM 980912, ITM 5114, and ITM 842) during infection of <i>A. polyphaga</i> at MOI 1.</p

Overview of intracellular and extracellular mycobacteria isolated from different habitats in BU endemic and non-endemic areas.

<p>Samples from which either the intracellular and/or extracellular cultures were contaminated, were discarded from this table and analyses. In the first and fourth column the number of samples from which we cultivated intracellular mycobacteria is given divided by the sample size. The same is presented in the second and fifth column, but with extracellular mycobacteria. In the third and sixth column, the relative isolation frequency of intracellular mycobacteria is given, calculated as the number of samples from which we cultivated intracellular mycobacteria divided by the total number of samples with mycobacteria cultures.</p

Light microscopic picture of <i>A. polyphaga</i> 3 hours after infection with <i>M. ulcerans</i> ITM 030216 (A), ITM 980912 (B), ITM 5114 (C), and ITM 842 (D).

<p>Light microscopic picture of <i>A. polyphaga</i> 3 hours after infection with <i>M. ulcerans</i> ITM 030216 (A), ITM 980912 (B), ITM 5114 (C), and ITM 842 (D).</p

Identified mycobacteria species isolated from intracellular and extracellular sources.

*<p>Based on 821 to 837 bp of the 16S rRNA-gene.</p

C_T values of samples and amoeba cultures positive for IS<i>2404</i> real-time PCR.

<p>ND = Not detected.</p>*<p>Based on 340 bp of the internal transcribed spacer, including the 5.8S rRNA gene.</p

Map of the study area [<b>63</b>].

<p>Map of the study area <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001764#pntd.0001764-Vandelannoote1" target="_blank">[<b>63</b>]</a>.</p

Isolation of FLA per type of specimen and sampling site.

<p>Isolation of FLA per type of specimen and sampling site.</p