4 research outputs found
Low circulating concentrations of citrulline and FGF19 predict chronic cholestasis and poor survival in adult patients with chronic intestinal failure: development of a Model for End-Stage Intestinal Failure (MESIF risk score)
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205171.pdf (publisher's version ) (Open Access)BACKGROUND: Patients with chronic intestinal failure (CIF) often develop cholestatic liver injury, which may lead to liver failure and need for organ transplantation. OBJECTIVES: The aim of this study was to investigate whether citrulline (CIT) and the enterokine fibroblast growth factor 19 (FGF19) are associated with chronic cholestasis and survival in adult CIF patients, and to develop a risk score to predict their survival. METHODS: We studied 135 adult CIF patients on intravenous supplementation (>3 mo). Associations of plasma CIT and FGF19 with chronic cholestasis and survival were estimated by logistic and Cox regression models. A predictive risk score was developed and validated internally. RESULTS: Patients with chronic cholestasis (17%) had a reduced 5-y survival rate compared with patients without chronic cholestasis (38% and 62%, respectively). In multivariable analysis, low FGF19, low CIT, and female sex were associated with chronic cholestasis. Patients with low rather than high CIT or FGF19 also had reduced 5-y survival rates (29% compared with 69%; 54% compared with 66%, respectively). Risk factors identified in multivariable analysis of survival were low FGF19 (HR: 3.4), low CIT (HR: 3.3), and number of intravenous infusions per week (HR: 1.4). These 3 predictors were incorporated in a risk model of survival termed Model for End-Stage Intestinal Failure (MESIF) (C-statistic 0.78). The 5-y survival rates for patients with MESIF scores of 0 to 40 (n = 13) were 80%, 58%, and 14%, respectively. CONCLUSIONS: CIT and FGF19 predict chronic cholestasis and survival in this cohort of adult CIF patients, and the derived MESIF score is associated with their survival. Pending external validation, the MESIF score may help to identify patients for closer clinical monitoring or earlier referral to intestinal transplantation centers
Elevated plasma levels of FGF19 is associated with repression of bile salt synthesis in patients with obstructive cholestasis
Bile Salt and FGF19 Signaling in the Early Phase of Human Liver Regeneration
The involvement of bile saltâfibroblast growth factor 19 (FGF19) signaling in human liver regeneration (LR) is not well studied. Therefore, we studied aspects of bile saltâFGF19 signaling shortly after liver resection in patients. We compared plasma bile salt and FGF19 levels in arterial, portal and hepatic venous blood, calculated venousâarterial differences (ÎVA), and determined hepatic transcript levels on two intraâoperative time points: before ( 2â3Â hours) liver resection (i.e., following surgery). Postoperative bile salt and FGF19 levels were assessed on days 1, 2, and 3. LR was studied by computed tomography (CT)âliver volumetry. Following surgery, the liver, arterial, and portal bile salt levels were elevated (PÂ <Â 0.05). Furthermore, an increased amount of bile salts was released in portal blood and extracted by the remnant liver (PÂ <Â 0.05). Postoperatively, bile salt levels were elevated from day 1 onward (PÂ <Â 0.001). For FGF19, intraâoperative or postoperative changes of ÎVA or plasma levels were not observed. The bile saltâhomeostatic regulator farnesoid X receptor (FXR) was markedly upâregulated following surgery (PÂ <Â 0.001). Cellâcycle reâentry priming factors (interleukin 6 [ILâ6], signal transducer and activator of transcription 3 [STAT3], and cJUN) were upâregulated following surgery and were positively correlated with FXR expression (PÂ <Â 0.05). Postoperative hyperbilirubinemia was preceded by postsurgery low FXR and high Na+/Taurocholate cotransporting polypeptide (NTCP) expression in the remnant liver coupled with higher liver bile salt content (PÂ <Â 0.05). Finally, bile salt levels on postoperative day 1 were an independent predictor of LR (PÂ <Â 0.05). Conclusion: Systemic, portal, and liver bile salt levels are rapidly elevated after liver resection. Postoperative bile salts were positively associated with liver volume gain. In the studied time frame, FGF19 levels remained unaltered, suggesting that FGF19 plays a minor role in human LR. These findings indicate a more relevant role of bile salts in human LR
Bile Salt and FGF19 Signaling in the Early Phase of Human Liver Regeneration
The involvement of bile saltâfibroblast growth factor 19 (FGF19) signaling in human liver regeneration (LR) is not well studied. Therefore, we studied aspects of bile saltâFGF19 signaling shortly after liver resection in patients. We compared plasma bile salt and FGF19 levels in arterial, portal and hepatic venous blood, calculated venousâarterial differences (ÎVA), and determined hepatic transcript levels on two intraâoperative time points: before ( 2â3Â hours) liver resection (i.e., following surgery). Postoperative bile salt and FGF19 levels were assessed on days 1, 2, and 3. LR was studied by computed tomography (CT)âliver volumetry. Following surgery, the liver, arterial, and portal bile salt levels were elevated (PÂ <Â 0.05). Furthermore, an increased amount of bile salts was released in portal blood and extracted by the remnant liver (PÂ <Â 0.05). Postoperatively, bile salt levels were elevated from day 1 onward (PÂ <Â 0.001). For FGF19, intraâoperative or postoperative changes of ÎVA or plasma levels were not observed. The bile saltâhomeostatic regulator farnesoid X receptor (FXR) was markedly upâregulated following surgery (PÂ <Â 0.001). Cellâcycle reâentry priming factors (interleukin 6 [ILâ6], signal transducer and activator of transcription 3 [STAT3], and cJUN) were upâregulated following surgery and were positively correlated with FXR expression (PÂ <Â 0.05). Postoperative hyperbilirubinemia was preceded by postsurgery low FXR and high Na+/Taurocholate cotransporting polypeptide (NTCP) expression in the remnant liver coupled with higher liver bile salt content (PÂ <Â 0.05). Finally, bile salt levels on postoperative day 1 were an independent predictor of LR (PÂ <Â 0.05). Conclusion: Systemic, portal, and liver bile salt levels are rapidly elevated after liver resection. Postoperative bile salts were positively associated with liver volume gain. In the studied time frame, FGF19 levels remained unaltered, suggesting that FGF19 plays a minor role in human LR. These findings indicate a more relevant role of bile salts in human LR