Genetic and phenotypic variation of Fusarium proliferatum isolates from different host species

Fusarium proliferatum (Matsushima) Nirenberg is a common pathogen infecting numerous crop plants and occurring in various climatic zones. It produces large amounts of fumonisins, a group of polyketide-derived mycotoxins. Fumonisin biosynthesis is determined by the presence and activity of the FUM cluster, several co-regulated genes with a common expression pattern. In the present work, we analyzed 38 F. proliferatum isolates from different host plant species, demonstrating host-specific polymorphisms in partial sequences of the key FUM1 gene (encoding polyketide synthase). We also studied growth rates across different temperatures and sample origin and tried to establish the relationships between DNA sequence polymorphism and toxigenic potential. Phylogenetic analysis was conducted based on FUM1 and tef-1α sequences for all isolates. The results indicated the greatest variations of both toxigenic potential and growth patterns found across the wide selection of isolates derived from maize. Fumonisin production for maize isolates ranged from 3.74 to 4,500 μg/g of fumonisin B1. The most efficient producer isolates obtained from other host plants were only able to synthesize 1,820–2,419 μg/g of this metabolite. A weak negative rank correlation between fumonisin content and isolate growth rates was observed. All garlic-derived isolates formed a distinct group on a FUM1-based dendrogram. A second clade consisted of tropical and sub-tropical strains (isolated from pineapple and date palm). Interestingly, isolates with the fastest growth patterns were also grouped together and included both isolates originating from rice. The sequence of the FUM1 gene was found to be useful in revealing the intraspecific polymorphism, which is, to some extent, specifically correlated with the host plant

Selection of thermophysical properties of heat storage wall

The paper discusses a method of the multilayered heat storage wall. Initial data: walls are multilayered, heat conductivity process is considered being one-dimensional, climatic factors and power supplied are periodic functions of time. In the paper mathematical and numerical models are presented. A mathematical model is constituted of a system of differential equations which describe heat conduction in material layers, equation of heat balance and boundary conditions system. Solving equations with regard to unknown property which has been recognized as a crucial parameter in the task one can obtain a real numeric value of the parameter. The paper is illustrated by the nomograms of calculated accumulating layer thickness and the scheme of an algorithm designed to calculate the thickness of accumulating layer. Frist Published Online: 30 Jul 201

Betriebsfestigkeit und Berechnung der Lebensdauer mit einer allgemeineren Darstellung der Wöhlerlinie

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Wölbkrafttorsion und Querkraftschubspannungen in einem Balken bei FE-Diskretisierung seines Querschnitts

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Lineare Viskoelastizität in differentieller Darstellung

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Berechnung dünner elastischer Platten mit finiten Elementen bei näherungsweiser Befriedigung der Kompatbilitätsbedingung

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Analysis of the possibilities to achieve the low energy residential buildings standards

The article applies the global costs calculation method to the energy assessment of selected solutions for the technical equipping of buildings, dependant on their purpose and function. Representative archetypes, the life span of which were assumed to be 30-years, were used to estimate the performance of the minimum requirements according to the building regulations valid in 2013, current general standards, as well as low energy building and passive house standards. The subjects of the analysis were three residential buildings, for which the variability of the cladding and ventilation needs was assumed. In all analysed cases the application of four systems of energy supply to the building was also assumed. The optimal level of primary energy index value, dependant on the source of heat, was established by means of the global cost method. This level was then compared with the requirements presently valid in Poland. The analysis also allowed for the determination of the growth in investment costs, that must be considered in order to achieve the energy needed for heating and ventilation at the level of 40 kWh/m2/a or 15 kWh/m2/a. The analysis presents a comparison of the subsidized loans for energy-efficient houses offered by the Polish Government. First published online: 23 Nov 201

FEM-Lösung des Problems der St.-Venantschen Torsion mit Hilfe der Wölbfunktion

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Structural solutions impact on thermal energy efficiency of heating surface at an open space

This paper presents studies on thermal energy efficiency of heating surface at an open space according to structural solutions and climatic conditions. Numerical simulation research was conducted to assess three different types of heating surfaces at an open space over chosen period of time in real weather conditions. Performance parameters such as surface temperature, supply temperature and efficiency of heating surface relative to constructional designs and model of control strategy used were analysed. The number, thickness and type of material layers beneath ground level were modified. The distance between heating pipes and their diameters were kept constant. The carried out analyses show that the used solutions can lead to significant differences in the performance and consequently in the energy efficiency of the heating system for open spaces

FEM-Software für Kleinrechner

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