Influence Of Corrosion On Lipopolysaccharide Affinity For Two Different Titanium Materials

Statement of problem Titanium is subject to corrosion in the oral cavity, which could contribute to periimplantitis. However, the effect of corrosion on the lipopolysaccharide affinity for titanium remains unknown. Purpose This study evaluated the role of corrosion (in artificial saliva at pHs 3, 6.5, and 9) on the lipopolysaccharide (LPS) affinity for commercially pure titanium (cp-Ti) and Ti-6Al-4V alloy. Material and methods Seventy-two titanium disks were anodically polarized in a controlled environment (n=9). Control specimens were not corroded. Deionized water with different concentrations of LPS (1.5, 15, and 150 μg/mL) were used to treat the disks for 24 hours to investigate LPS adherence (n=3). Then specimens were immersed in LPS-free water to evaluate LPS elution at 24, 48, and 72 hours. Data were analyzed by the 2-way, 3-way, and 3-way repeated measures ANOVA, t tests, and the Tukey honestly significant difference (HSD) tests (α=.05). Results A greater corrosion rate of cp-Ti and Ti-6Al-4V alloy and a higher LPS adherence to titanium surfaces (P<.05) were noted at acidic pH. The LPS affinity was higher for the Ti-6Al-4V alloy than for cp-Ti (P<.05). More LPS was eluted from titanium surfaces after a 24-hour interval. Conclusion Lipopolysaccharide affinity for cp-Ti and Ti-6Al-4V alloy is influenced by the corrosion process.1106462470Nakagawa, M., Matsuya, S., Udoh, K., Effects of fluoride and dissolved oxygen concentrations on the corrosion behavior of pure titanium and titanium alloys (2002) Dent Mater J, 21, pp. 83-92Gil, F.J., Canedo, R., Padros, A., Baneres, M.V., Arano, J.M., Fretting corrosion behaviour of ball-and-socket joint on dental implants with different prosthodontic alloys (2003) Biomed Mater Eng, 13, pp. 27-34Nakagawa, M., Matsuya, S., Shiraishi, T., Ohta, M., Effect of fluoride concentration and pH on corrosion behavior of titanium for dental use (1999) J Dent Res, 78, pp. 1568-1572Nikolopoulou, F., Saliva and dental implants (2006) Implant Dent, 15, pp. 372-376Vieira, A.C., Ribeiro, A.R., Rocha, L.A., Celis, J.P., Influence of pH and corrosion inhibitors on the tribocorrosion of titanium in artificial saliva (2006) Wear, 261, pp. 994-1001Azzi, M., Szpunar, J.A., Tribo-electrochemical technique for studying tribocorrosion behavior of biomaterials (2007) Biomol Eng, 24, pp. 443-446De Assis, S.L., Wolynec, S., Costa, I., Corrosion characterization of titanium alloys by electrochemical techniques (2006) Electrochim Acta, 51, pp. 1815-1819Mabilleau, G., Bourdon, S., Joly-Guillou, M.L., Filmon, R., Basle, M.F., Chappard, D., Influence of fluoride, hydrogen peroxide and lactic acid on the corrosion resistance of commercially pure titanium (2006) Acta Biomater, 2, pp. 121-129Souza, M.E., Lima, L., Lima, C.R., Zavaglia, C.A., Freire, C.M., Effects of pH on the electrochemical behaviour of titanium alloys for implant applications (2009) J Mater Sci Mater Med, 20, pp. 549-552Huang, H.H., Effect of fluoride and albumin concentration on the corrosion behavior of Ti-6Al-4V alloy (2003) Biomaterials, 24, pp. 275-282Correa, C.B., Pires, J.R., Fernandes-Filho, R.B., Sartori, R., Vaz, L.G., Fatigue and fluoride corrosion on Streptococcus mutans adherence to titanium-based implant/component surfaces (2009) J Prosthodont, 18, pp. 382-387Joska, L., Fojt, J., Corrosion behaviour of titanium after short-term exposure to an acidic environment containing fluoride ions (2010) J Mater Sci Mater Med, 21, pp. 481-488Quirynen, M., De Soete, M., Van Steenberghe, D., Infectious risks for oral implants: A review of the literature (2002) Clin Oral Implants Res, 13, pp. 1-19Morgan, T.D., Wilson, M., The effects of surface roughness and type of denture acrylic on biofilm formation by Streptococcus oralis in a constant depth film fermentor (2001) J Appl Microbiol, 91, pp. 47-53Bollen, C.M., Papaioanno, W., Van Eldere, J., Schepers, E., Quirynen, M., Van Steenberghe, D., The influence of abutment surface roughness on plaque accumulation and peri-implant mucositis (1996) Clin Oral Implants Res, 7, pp. 201-211Quirynen, M., Bollen, C.M., Willems, G., Van Steenberghe, D., Comparison of surface characteristics of six commercially pure titanium abutments (1994) Int J Oral Maxillofac Implants, 9, pp. 71-76Simon, B.I., Goldman, H.M., Ruben, M.P., Baker, E., The role of endotoxin in periodontal disease. I. A reproducible, quantitative method for determining the amount of endotoxin in human gingival exudate (1969) J Periodontol, 40, pp. 695-701Wilson, M., Biological activities of lipopolysaccharides from oral bacteria and their relevance to the pathogenesis of chronic periodontitis (1995) Sci Prog, 78 (PART 1), pp. 19-34Quirynen, M., Vogels, R., Peeters, W., Van Steenberghe, D., Naert, I., Haffajee, A., Dynamics of initial subgingival colonization of 'pristine' peri-implant pockets (2006) Clin Oral Implants Res, 17, pp. 25-37Giannelli, M., Bani, D., Tani, A., Pini, A., Margheri, M., Zecchi-Orlandini, S., In vitro evaluation of the effects of low-intensity Nd:YAG laser irradiation on the inflammatory reaction elicited by bacterial lipopolysaccharide adherent to titanium dental implants (2009) J Periodontol, 80, pp. 977-984Nelson, S.K., Knoernschild, K.L., Robinson, F.G., Schuster, G.S., Lipopolysaccharide affinity for titanium implant biomaterials (1997) J Prosthet Dent, 77, pp. 76-82Knoernschild, K.L., Tompkins, G.R., Schuster, G.S., Lefebvre, C.A., Russell, C.M., Effect of treatment concentration on lipopolysaccharide affinity for two alloys (1997) Dent Mater, 13, pp. 111-117Knoernschild, K.L., Bacon, W.L., Fischman, G.S., Campbell, S.D., Effect of pH on endotoxin affinity for metal-ceramic alloys (2001) J Prosthet Dent, 86, pp. 644-649Knoernschild, K.L., Lefebvre, C.A., Schuster, G.S., Payant, L.M., Gagnon, F.M., Endotoxin adherence to and elution from two casting alloys (1994) Int J Prosthodont, 7, pp. 22-29Knoernschild, K.L., Tompkins, G.R., Lefebvre, C.A., Schuster, G.S., Porphyromonas gingivalis lipopolysaccharide affinity for two casting alloys (1995) J Prosthet Dent, 74, pp. 33-38Gagnon, F., Knoernschild, K.L., Payant, L., Tompkins, G.R., Litaker, M.S., Schuster, G.S., Endotoxin affinity for provisional restorative resins (1994) J Prosthodont, 3, pp. 228-236Knoernschild, K.L., Tompkins, G.R., Lefebvre, C.A., Griffiths, L.L., Schuster, G.S., Effect of pH on Porphyromonas gingivalis endotoxin affinity for resins (1996) Int J Prosthodont, 9, pp. 239-247Robinson, F.G., Knoernschild, K.L., Sterrett, J.D., Tompkins, G.R., Porphyromonas gingivalis endotoxin affinity for dental ceramics (1996) J Prosthet Dent, 75, pp. 217-227Barao, V.A., Mathew, M.T., Assuncao, W.G., Yuan, J.C., Wimmer, M.A., Sukotjo, C., The role of lipopolysaccharide on the electrochemical behavior of titanium (2011) J Dent Res, 90, pp. 613-618Mathew, M.T., Barao, V.A., Yuan, J.C., Assuncao, W.G., Sukotjo, C., Wimmer, M.A., What is the role of lipopolysaccharide on the tribocorrosive behavior of titanium? (2012) J Mech Behav Biomed Mater, 8, pp. 71-85Barao, V.A., Mathew, M.T., Assuncao, W.G., Yuan, J.C., Wimmer, M.A., Sukotjo, C., Stability of cp-Ti and Ti-6Al-4V alloy for dental implants as a function of saliva pH - An electrochemical study (2012) Clin Oral Implants Res, 23, pp. 1055-1062(2009), http://www.astm.org/Standard/index.shtml, American Society for Testing and Materials. ASTM-G61-G86. Standard Test Method for Conducting Cyclic Potentiodynamic Polarization Measurements for Localized Corrosion Susceptibility of Iron-, Nickel-, or Cobalt-Based Alloys. West Conshohocken, Pa: ASTM(2004), http://www.astm.org/Standard/index.shtml, American Society for Testing and Materials. ASTM-G31-G72. Standard practice for laboratory immersion corrosion testing of metals. West Conshohocken, Pa: ASTMLiu, I.H., Lee, T.M., Chang, C.Y., Liu, C.K., Effect of load deflection on corrosion behavior of NiTi wire (2007) J Dent Res, 86, pp. 539-543Baboian, R., (2005) Corrosion Tests and Standards: Application and Interpretation, pp. 107-130. , 2nd ed. ASTM International West Conshohocke