Early detection of disease program: Evaluation of the cellular immune response

Surfaces of normal, cultured, and mitogen-stimulated mouse lymphoid cells were examined by scanning electron microscopy (SEM). Lymphocytes with smooth, highly villous and intermediate surfaces were observed in cell suspensions from both spleens and thymuses of normal mice and from spleens of congenitally athymic (nude) mice. Several strain-specific surface features were noted, including the spine-like appearance of microvilli on C57B1/6 lymphocytes. Although thymus cell suspensions contained somewhat more smooth cells than did spleen cell preparations, lymphocyte derivation could not be inferred from SEM examination. Studies of cells stimulated with mitogenic agents for thymus-derived lymphocytes (concanavalin A) or for bone marrow-derived lymphocytes (lipopolysaccharide) suggested that, in the mouse, development of a complex villous surface is a general concomitant of lymphocyte activation and transformation

Spectra and positions of galactic gamma-ray sources

The UCSD/MIT Hard X-Ray and Low Energy Gamma-Ray Experiment aboard HEAO-1 scanned the galactic center region during three epochs in 1977 and 1978 from 13 to 180 keV. The results are presented from the scanning epoch of 1978 September. Twenty-two known 2 to 10 keV source positions were necessary for an acceptable fit to the data. The spectra of the 16 strongest, least confused sources are all consistent with power laws with photon spectral indices ranging from 2.1 to 7.2. Acceptable fits to thermal bremsstrahlung models are also possible for most sources. No one source in this survey can be extrapolated to higher energy to match the intensity of the gamma-ray continuum as measured by HEAO-1 large field of view detectors, which implies that the continuum is a composite of contributions from a number of sources

Genomic delineation of zoonotic origins of Clostridium difficile

Clostridium difficile is toxin-producing antimicrobial resistant (AMR) enteropathogen historically associated with diarrhea and pseudomembranous colitis in hospitalized patients. In recent years, there have been dramatic increases in the incidence and severity of C. difficile infection (CDI), and associated morbidity and mortality, in both healthcare and community settings. C. difficile is an ancient and diverse species that displays a sympatric lifestyle, establishing itself in a range of ecological niches external to the healthcare system. These sources/reservoirs include food, water, soil, and over a dozen animal species, in particular, livestock such as pigs and cattle. In a manner analogous to human infection, excessive antimicrobial exposure, particularly to cephalosporins, is driving the expansion of C. difficile in livestock populations worldwide. Subsequent spore contamination of meat, vegetables grown in soil containing animal feces, agricultural by-products such as compost and manure, and the environment in general (households, lawns, and public spaces) is contributing to a persistent community source/reservoir of C. difficile and the insidious rise of CDI in the community. The whole-genome sequencing era continues to redefine our view of this complex pathogen. The application of high-resolution microbial genomics in a One Health framework (encompassing clinical, veterinary, and environment derived datasets) is the optimal paradigm for advancing our understanding of CDI in humans and animals. This approach has begun to yield critical insights into the genetic diversity, evolution, AMR, and zoonotic potential of C. difficile. In Europe, North America, and Australia, microevolutionary analysis of the C. difficile core genome shows strains common to humans and animals (livestock or companion animals) do not form distinct populations but share a recent evolutionary history. Moreover, for C. difficile sequence type 11 and PCR ribotypes 078 and 014, major lineages of One Health importance, this approach has substantiated inter-species clonal transmission between animals and humans. These findings indicate either a zoonosis or anthroponosis. Moreover, they challenge the existing paradigm and the long-held misconception that CDI is primarily a healthcare-associated infection. In this article, evolutionary, and zoonotic aspects of CDI are discussed, including the anthropomorphic factors that contribute to the spread of C. difficile from the farm to the community

A comparative analysis of rawinsonde and NIMBUS 6 and TIROS N satellite profile data

Comparisons are made between rawinsonde and satellite profiles in seven areas for a wide range of surface and weather conditions. Variables considered include temperature, dewpoint temperature, thickness, precipitable water, lapse rate of temperature, stability, geopotential height, mixing ratio, wind direction, wind speed, and kinematic parameters, including vorticity and the advection of vorticity and temperature. In addition, comparisons are made in the form of cross sections and synoptic fields for selected variables. Sounding data from the NIMBUS 6 and TIROS N satellites were used. Geostrophic wind computed from smoothed geopotential heights provided large scale flow patterns that agreed well with the rawinsonde wind fields. Surface wind patterns as well as magnitudes computed by use of the log law to extrapolate wind to a height of 10 m agreed with observations. Results of this study demonstrate rather conclusively that satellite profile data can be used to determine characteristics of large scale systems but that small scale features, such as frontal zones, cannot yet be resolved

Kinetic pinning and biological antifreezes

Biological antifreezes protect cold-water organisms from freezing. An example are the antifreeze proteins (AFPs) that attach to the surface of ice crystals and arrest growth. The mechanism for growth arrest has not been heretofore understood in a quantitative way. We present a complete theory based on a kinetic model. We use the `stones on a pillow' picture. Our theory of the suppression of the freezing point as a function of the concentration of the AFP is quantitatively accurate. It gives a correct description of the dependence of the freezing point suppression on the geometry of the protein, and might lead to advances in design of synthetic AFPs.Comment: 4 pages, 4 figure

Fast light, slow light, and phase singularities: a connection to generalized weak values

We demonstrate that Aharonov-Albert-Vaidman (AAV) weak values have a direct relationship with the response function of a system, and have a much wider range of applicability in both the classical and quantum domains than previously thought. Using this idea, we have built an optical system, based on a birefringent photonic crystal, with an infinite number of weak values. In this system, the propagation speed of a polarized light pulse displays both superluminal and slow light behavior with a sharp transition between the two regimes. We show that this system's response possesses two-dimensional, vortex-antivortex phase singularities. Important consequences for optical signal processing are discussed.Comment: 9 pages, 4 figures, accepted in Physical Review Letters (2003

Distribution of chirality in the quantum walk: Markov process and entanglement

The asymptotic behavior of the quantum walk on the line is investigated focusing on the probability distribution of chirality independently of position. The long-time limit of this distribution is shown to exist and to depend on the initial conditions, and it also determines the asymptotic value of the entanglement between the coin and the position. It is shown that for given asymptotic values of both the entanglement and the chirality distribution it is possible to find the corresponding initial conditions within a particular class of spatially extended Gaussian distributions. Moreover it is shown that the entanglement also measures the degree of Markovian randomness of the distribution of chirality.Comment: 5 pages, 3 figures, It was accepted in Physcial Review

Cytometric analysis, genetic manipulation and antibiotic selection of the snail embryonic cell line Bge from Biomphalaria glabrata, the intermediate host of Schistosoma mansoni.

The invertebrate cell line, Bge, from embryos of the snail Biomphalaria glabrata, remains to date the only established cell line from any species of the Phylum Mollusca. Since its establishment in 1976 by Eder Hansen, few studies have focused on profiling its cytometrics, growth characteristics or sensitivity to xenobiotics. Bge cells are reputed to be challenging to propagate and maintain. Therefore, even though this cell line is a noteworthy resource, it has not been studied widely. With growing interest in functional genomics, including genetic transformation, to elucidate molecular aspects of the snail intermediate hosts responsible for transmission of schistosomiasis, and aiming to enhance the convenience of maintenance of this molluscan cell line, we deployed the xCELLigene real time approach to study Bge cells. Doubling times for three isolates of Bge, termed CB, SL and UK, were longer than for mammalian cell lines - longer than 40 h in complete Bge medium supplemented with 7% fetal bovine serum at 25 °C, ranging from ∼42 h to ∼157 h when 40,000 cells were seeded. To assess the potential of the cells for genetic transformation, antibiotic selection was explored. Bge cells were sensitive to the aminonucleoside antibiotic puromycin (from Streptomyces alboniger) from 5 μg/ml to 200 ng/ml, displaying a half maximal inhibitory concentration (IC50) of ∼1.91 μg/ml. Sensitivity to puromycin, and a relatively quick kill time (<48 h in 5 μg/ml) facilitated use of this antibiotic, together with the cognate resistance gene (puromycin N-acetyl-transferase) for selection of Bge cells transformed with the PAC gene (puroR). Bge cells transfected with a plasmid encoding puroR were partially rescued when cultured in the presence of 5 μg/ml of puromycin. These findings pave the way for the development of functional genomic tools applied to the host-parasite interaction during schistosomiasis and neglected tropical trematodiases at large

Complete genome assemblies of three highly prevalent, toxigenic clostridioides difficile strains causing health care-associated infections in Australia

Clostridioides difficile infection (CDI) is the leading cause of life-threatening health care-related gastrointestinal illness worldwide. Phylogenetically appropriate closed reference genomes are essential for studies of C. difficile transmission and evolution. Here, we provide high-quality complete hybrid genome assemblies for the three most prevalent C. difficile strains causing CDI in Australia