Construir lo colectivo en la Reserva Los Quebrachitos, Unquillo

Trabajo final de gradoLa Reserva Natural, Hídrica y Recreativa "Los Quebrachitos), ocupa hoy en la provincia de Córdoba y en particular en el corredor ambiental, social y económico de Sierras Chicas, un lugar significativo como ícono de resistencia ante las lógicas de mercado que se imponen sobre los intereses colectivos y el ambiente. La reserva está enmarcada en la especulación del valor del paisaje lo cual, por un lado, atenta contra su identidad y, por otro, genera un escenario enmarcado por situaciones problemáticas y conflictivas como: escasas políticas estatáles de cuidado y protección del área protegida; expansión de la fontera urbana, minera y agro exportadora sobre el bosque nativo; crecimiento poblacional del área urbanizada por extensión; privatización del acceso a los espaccios comunes de la reserva; deforestación, pérdida de flora y fauna nativa, introducción de especies exóticas; impermeabilización del suelo con secuelas como inundaciones, incendios, sequías. Por ello, sostenemos que se produce una interface entre situaciones naturales y antrópicas, y dentro de este marco, una incetidumbre por el derecho a un hábitat digno en términos ambientales, sociales y culturales para lograr un equilibrio sostenible. La propuesta de trabajo buscó interpretar algunas de las miradas que existen sobre la confuguración del territorio (vecinos, organizaciones e instituciones barriales), generar procesos de aprendizaje dialógicos y reforzar vínculos entre las partes involucradas a partir del diseño de un plan de acción y diseño participativo de los espacios de uso común a efectos de empoderar a los actores de la reserva. Creemos urgente y necesario comprometernos con este tipo de problemáticas territoriales desde la Universidad, aportando herramientas técnicas y creando nuevos canales de discusión dentro de las organizaciones. Además, que el conocimiento académico abandone el hermetismo de las aulas para entrar para entrar en diálogo con los saberes populares y pasemos, por fin, dela idea a la acción en los complejos escenarios de lo real al poner el cuerpo a las dinámicas cambiantes del territori

Kinder- und Jugendöffentlichkeit: Entstehung, Implikationen und Rahmenbedingungen einer kindheitswissenschaftlichen Kategorie

Thema des Beitrags ist das auf den Kinderrechten basierende Konzept der Kinderöffentlichkeit und was darunter verstanden werden kann. Der Aufsatz präsentiert Beispiele, wie über Kinder, mit Kindern sowie von Kindern und Jugendlichen Öffentlichkeit hergestellt wird. Damit verbunden begründet der Text, warum und wie Kinder ihre Bedürfnisse und ihre Meinungen öffentlich äußern können, sodass diese auch berücksichtigt werden. Sodann findet auch die Rolle von Erwachsenen Berücksichtigung, wenn es gilt, Kinder zu unterstützen, ohne sie zu bevormunden. Räume, Zeiten und soziale Lebenslagen lassen sich als zentrale Einflussfaktoren für Kinder- und Jugendöffentlichkeit ausmachen. Schließlich geht es darum, mit (kinder-)politikwissenschaftlicher Perspektive gesellschaftspolitische Rahmenbedingungen für Kinder- und Jugendöffentlichkeit zu erkunden.The article deals with Children’s rights-based concepts of youth publicity. Chosen examples of publicity about children, with children and by children can show different meanings of youth publicity. Accordingly the text asks why and how children shall express their best interests publicly so that they are realy heard. Thus the role of adults must be considered supporting children in processes of participation without tutelage. Public areas, spare times and social living conditions are determinant for children and youth publicity. Finally the socio-political circumstances of children and youth publicity must be examined in perspective of children policy

Site directed Mutagenesis of Benzophenonesynthase of Hypericum androsaemum

In dieser Arbeit wurden Untersuchungen des Reaktionsablaufs zu dem Enzym Benzophenonsynthase (BPS) durchgeführt. Dieses Enzym akzeptiert als Startersubstrat Benzoyl-CoA und verlängert dieses mit drei C2-Einheiten aus Malonyl-CoA zu einem Tetraketid. Nach anschließender Zyklisierung sowie einer Claisen-Kondensation entsteht das Hauptprodukt 2,4,6-Trihydroxybenzophenon. Die BPS wurde in E. coli exprimiert und mit Hilfe von molekularbiologischen Methoden eine Reihe von Punktmutationen eingeführt. Durch Punktmutation gelang es eine Enzymmutante zu erstellen, die eine veränderte Substrat- und Produktspezifität aufweist. Sie wurde so verändert, dass an der Aminosäurensequenzposition 135 ein Threonin gegen ein Leucin ausgetauscht wurde. Die Produktspezifitätsänderung zeigt sich dadurch, dass diese Mutante nach Inkubation mit Benzoyl-CoA und Kettenverlängerung mit Malonyl-CoA nicht mehr das Tetraketid Benzophenon bildet, sondern nur noch ein Triketid, welches zum Phenylpyron laktonisiert wird. Zusätzlich gelang es durch diese Mutation die Substratspezifität so zu ändern, dass für das Substrat 3-Hydroxybenzoyl-CoA, welches zuvor hohe Aktivität mit dem Wild-Typ aufwies, keine Aktivität mehr gezeigt werden konnte. Durch Homologiemodellierung auf der Basis der Röntgenstruktur der verwandten Chalkonsynthase aus Medicago sativa konnten Rückschlüsse auf den nun veränderten Reaktionsablauf im aktiven Zentrum des Enzyms sowohl im Wild-Typ als auch in der Mutante gezogen werden. Im Strukturmodell der Mutante T135L wurde eine zusätzliche Bindetasche identifiziert, die in der Lage ist, das entstehende Triketid mit hoher Affinität zu binden und so die weitere Kettenverlängerung zu verhindern. Durch anschließende kinetische Charakterisierung zeigte sich, dass sowohl die Benzophenonsynthase als Wild-Typ als auch ihre Mutante vergleichbare katalytische Effizienzen besitzen und somit gezeigt werden konnte, dass eine Benzophenonsynthase in eine Phenylpyronsynthase umgewandelt werden kann.In this work the reaction mechanism of the enzyme benzophenone synthase (BPS) has been investigated. This enzyme accepts Benzoyl-CoA as the main starting substrate which is elongated with three C2 units from malonyl-CoA to form a tetraketide. After a subsequent cyclization and claisen condensation the main product 2,4,6-trihydroxybenzophenone is formed. The BPS was expressed in E. coli and a series of point mutations was introduced by molecular biology techniques. By taking this approach a mutation of the enzyme with different substrate und product specificity was created, replacing threonine by leucin in position 135. The mutant shows different product specificity: the tetraketide related benzophenone was no longer formed after incubation with benzoyl-CoA and chain elongation with malonyl-CoA but a triketide that leads to the formation of phenylpyrone after a lactonization step. In addition to that the mutant showed no measurable activity with 3-hydroxybenzoyl-CoA, which was an efficient substrate for the wild-type enzyme. Based on the x-ray crystal structure of the related chalcone synthase from Medicago sativa a homology model was derived to draw conclusions on the altered reaction mechanism in the catalytic centre of the mutant relative to the wild- type. An additional binding pocket is identified in the catalytic centre of the mutant T135L. It binds the intermediate triketide with high affinity and prevents further elongation steps. The subsequent characterization of the kinetic parameters proves that wild-type and mutant T135L to have similar catalytic efficiency and illustrates the ability of turning a benzophenone synthase into a phenylpyrone synthase by a single point mutation

»Saving Capitalism from itself«? Entstehung und Entwicklung sozialdemokratischer Wohlfahrtsstaatlichkeit

»Saving Capitalism from itself«? Entstehung und Entwicklung sozialdemokratischer Wohlfahrtsstaatlichkei

Using the Sirocco File System for high-bandwidth checkpoints.

The Sirocco File System, a file system for exascale under active development, is designed to allow the storage software to maximize quality of service through increased flexibility and local decision-making. By allowing the storage system to manage a range of storage targets that have varying speeds and capacities, the system can increase the speed and surety of storage to the application. We instrument CTH to use a group of RAM-based Sirocco storage servers allocated within the job as a high-performance storage tier to accept checkpoints, allowing computation to potentially continue asynchronously of checkpoint migration to slower, more permanent storage. The result is a 10-60x speedup in constructing and moving checkpoint data from the compute nodes. This demonstration of early Sirocco functionality shows a significant benefit for a real I/O workload, checkpointing, in a real application, CTH. By running Sirocco storage servers within a job as RAM-only stores, CTH was able to store checkpoints 10-60x faster than storing to PanFS, allowing the job to continue computing sooner. While this prototype did not include automatic data migration, the checkpoint was available to be pushed or pulled to disk-based storage as needed after the compute nodes continued computing. Future developments include the ability to dynamically spawn Sirocco nodes to absorb checkpoints, expanding this mechanism to other fast tiers of storage like flash memory, and sharing of dynamic Sirocco nodes between multiple jobs as needed

Extending the Posix I/O Interface: A Parallel File System Perspective.

The POSIX interface does not lend itself well to enabling good performance for high-end applications. Extensions are needed in the POSIX I/O interface so that high-concurrency HPC applications running on top of parallel file systems perform well. This paper presents the rationale, design, and evaluation of a reference implementation of a subset of the POSIX I/O interfaces on a widely used parallel file system (PVFS) on clusters. Experimental results on a set of micro-benchmarks confirm that the extensions to the POSIX interface greatly improve scalability and performance

Effects of Anacetrapib in Patients with Atherosclerotic Vascular Disease

BACKGROUND: Patients with atherosclerotic vascular disease remain at high risk for cardiovascular events despite effective statin-based treatment of low-density lipoprotein (LDL) cholesterol levels. The inhibition of cholesteryl ester transfer protein (CETP) by anacetrapib reduces LDL cholesterol levels and increases high-density lipoprotein (HDL) cholesterol levels. However, trials of other CETP inhibitors have shown neutral or adverse effects on cardiovascular outcomes. METHODS: We conducted a randomized, double-blind, placebo-controlled trial involving 30,449 adults with atherosclerotic vascular disease who were receiving intensive atorvastatin therapy and who had a mean LDL cholesterol level of 61 mg per deciliter (1.58 mmol per liter), a mean non-HDL cholesterol level of 92 mg per deciliter (2.38 mmol per liter), and a mean HDL cholesterol level of 40 mg per deciliter (1.03 mmol per liter). The patients were assigned to receive either 100 mg of anacetrapib once daily (15,225 patients) or matching placebo (15,224 patients). The primary outcome was the first major coronary event, a composite of coronary death, myocardial infarction, or coronary revascularization. RESULTS: During the median follow-up period of 4.1 years, the primary outcome occurred in significantly fewer patients in the anacetrapib group than in the placebo group (1640 of 15,225 patients [10.8%] vs. 1803 of 15,224 patients [11.8%]; rate ratio, 0.91; 95% confidence interval, 0.85 to 0.97; P=0.004). The relative difference in risk was similar across multiple prespecified subgroups. At the trial midpoint, the mean level of HDL cholesterol was higher by 43 mg per deciliter (1.12 mmol per liter) in the anacetrapib group than in the placebo group (a relative difference of 104%), and the mean level of non-HDL cholesterol was lower by 17 mg per deciliter (0.44 mmol per liter), a relative difference of -18%. There were no significant between-group differences in the risk of death, cancer, or other serious adverse events. CONCLUSIONS: Among patients with atherosclerotic vascular disease who were receiving intensive statin therapy, the use of anacetrapib resulted in a lower incidence of major coronary events than the use of placebo. (Funded by Merck and others; Current Controlled Trials number, ISRCTN48678192 ; ClinicalTrials.gov number, NCT01252953 ; and EudraCT number, 2010-023467-18 .)

The Role of Lean and Agile Supply Attributes in the Performance of VAS in Distribution Centres: An Exploratory Study

Background: The integration of different customised value added services (VAS) in warehouse logistics is an increasing trend. This fact requires logistics managers to identify the most appropriate strategies to meet VAS customer demands and to achieve a superior performance over their competitors. However, as VAS are heterogeneous in nature, the customer demand of these services can vary accordingly. Therefore, distribution centres need to develop those supply attributes, which enable them to respond to each particular VAS customer demand. In this context, the choice between lean and agile supply attributes, which are two contradictory concepts, comes especially into focus. The role of such concepts has been broadly introduced in the context of supply chain performance in the Fisher’s framework (1997) and the following models from different researchers. However, the issue of VAS performance in distribution centres under the perspectives of the concepts of lean and agile supply attributes was not considered in the previous warehouse logistics research. Research Aims: This study explored the topic in question from the perspective of distribution centres. The purpose of this study was to gain a greater insight of VAS applications, and particularly, to understand the contributions of lean and agile supply attributes in the performance of VAS in distribution centres. In particular, the study aimed to reframe the meaning of applications of VAS from the perspectives of logistics service providers, to identify the supply attributes necessary to meet VAS customer demands and to understand how managers of distribution centres align their operational strategies with the VAS performance. Methodology: As the research purpose was explorative in their nature, the empirical process in this study was underpinned by the perspectives of the phenomenological interpretivist paradigm. Thus, a qualitative case study of six distribution centres (six companies) in Germany dealing with VAS was carried out. The data was collected by semi-structured interviews, built on the triangulation of sources and use of quota sampling technique. In doing so, the head manager of distribution centre, the operational manager and the customer manager from each distribution centre were included in the research participant circle. The following analysis of the obtained data was based on the principles of Grounded Analysis method that implied an examination of the data by open, axial and selective coding procedures. Results: The main conclusion of this study is that the nature of customer demands of such VAS can vary quite a lot depending on the particular business cases and therefore, the concepts of lean and agile supply attributes have a crucial meaning in the context of VAS performance in distribution centres. This became especially true as, some distribution centres associate VAS performance with the lean concepts of “standards” and “productivity”, while the others link performance of VAS with the agile concepts of “flexibility” and “customer responsiveness”. In addition, the concept of “quality” has a special significance in the context of VAS performance regardless of the nature of customer demand. However, as shown by the analysis of empirical data, concepts of “quality” as well as “customer responsiveness” are not adequately considered in distribution centres. Furthermore, the research findings indicated that the “material-related” supplementary activities, which logistics service providers physically perform in the operations of their distribution centres in order to gain financial benefits, are the dominant model of VAS in the modern warehouse logistics. Moreover, the applications of such VAS with more agile nature of customer demand can bring higher financial benefit and different non-financial positive effects, but at the same time can lead to higher warehouse complexity. Implications: This study addressed the research gaps by providing a greater understanding of contributions of lean and agile supply attributes in the performance of VAS in distribution centres. In this respect, this study indicated that the perspectives of Fisher’s framework (1997) have high relevance in the particular context of VAS performance in modern warehouse logistics. Moreover, the study identified the significance of different concepts related to the VAS warehouse business such as “lack of non-physical VAS concepts”, “peculiarity of non TPL DCs”, “issue of quality” and “deficit of customer responsiveness measurements”, which need to be further explored by future research