40 research outputs found
Olefin Autoxidation in Flow
Handling hazardous multiphase reactions
in flow brings not only
safety advantages but also significantly improved performance, due
to better mass transfer characteristics. In this paper, we present
a continuous microreactor setup, capable of performing olefin autoxidations
with O<sub>2</sub>, under solvent-free and catalyst-free conditions.
Owing to the transparent reactor design, consumption of O<sub>2</sub> can be visually followed and exhaustion of the gas bubbles marks
a clear end point along the channel length coordinate. Tracking the
position of this end point enables measuring effective rate constants.
The developed system was calibrated using the well-studied β-pinene
substrate, and was subsequently applied to the synthetically interesting
transformation of (+)-valencene to (+)-nootkatone. For the latter,
a space-time yield was obtained that is at least 3 orders of magnitude
larger than that realized with established biotechnology approaches
Mass Transport and Reactions in the Tube-in-Tube Reactor
The
tube-in-tube reactor is a convenient method for implementing
gas/liquid reactions on the microscale, in which pressurized gas permeates
through a Teflon AF-2400 membrane and reacts with substrates in liquid
phase. Here we present the first quantitative models for analytically
and numerically computing gas and substrate concentration profiles
within the tube-in-tube reactor. The model accurately predicts mass
transfer performance in good agreement with experimental measurement.
The scaling behavior and reaction limitations of the tube-in-tube
reactor are predicted by modeling and compared with gas/liquid micro-
and minireactors. The presented model yields new insights into the
scalability and applicability of the tube-in-tube reactor
Microfluidic Continuous Seeded Crystallization: Extraction of Growth Kinetics and Impact of Impurity on Morphology
We describe a continuous microfluidic
system for seeded crystallization
of small organic molecules, such as active pharmaceuticals, and demonstrate
integration with in situ detection tools for determining the size
and polymorphic form of the crystals. This integrated device is used
to extract growth kinetics, as a screening platform for process parameter
effects and optimization, and to gain insight into the fundamentals
of the crystallization process. In addition, the microfluidic system
also allows studies of additive effects on the crystal habit. The
method is demonstrated with growth kinetics for α-, β-,
and γ-forms of glycine along with the effects upon the morphology
of adding glutamic acid and methionine
A pH-Sensitive Laser-Induced Fluorescence Technique To Monitor Mass Transfer in Multiphase Flows in Microfluidic Devices
We present a pH-sensitive laser-induced fluorescence
(LIF) technique
to investigate mass transfer in reactive flows. As a fluorescent dye,
we used 5-(and-6)-carboxy SNARF-1, which, when excited with a pulsed
Nd:YAG laser at 532 nm, provides good sensitivity in the range 4 ≤
pH ≤ 12. For validation, we first applied the dye to single-phase
reactive flows by investigating the neutralization of sodium hydroxide
with hydrochloric acid. Comparison to the classical passive mixing
case showed that this dye was able to capture the reaction progress
and to quantify the mass transport. Next, we investigated the absorption
of CO<sub>2</sub> in an alkaline solution using gas–liquid
flow and found that the LIF technique is able to quantify the local
mass-transfer rate in microfluidic systems. Results for different
microchannel geometries highlight the strong connection between local
mass transfer and secondary flow structures in gas–liquid Taylor
flow
An Automated Continuous-Flow Platform for the Estimation of Multistep Reaction Kinetics
Automated continuous flow systems coupled with online
analysis
and feedback have been previously demonstrated to model and optimize
chemical syntheses with little <i>a priori</i> reaction
information. However, these methods have yet to address the challenge
of modeling and optimizing for product yield or selectivity in a multistep
reaction network, where low selectivity toward desired product formation
can be encountered. Here we demonstrate an automated system capable
of rapidly estimating accurate kinetic parameters for a given reaction
network using maximum likelihood estimation and a <i>D</i>-optimal design of experiments. The network studied is the series–parallel
nucleophilic aromatic substitution of morpholine onto 2,4-dichloropyrimidine.
To improve the precision of the estimated parameters, we demonstrate
the use of the automated platform first in optimization of the yield
of the less kinetically favorable 2-substituted product. Then, upon
isolation of the intermediates, we use the automated system with maximum <i>a posteriori</i> estimation to minimize uncertainties in the
network parameters. From considering the steps of the reaction network
in isolation, the kinetic parameter uncertainties are reduced by 50%,
with less than 5 g of the dichloropyrimidine substrate consumed over
all experiments. We conclude that isolating pathways in the multistep
reaction network is important to minimizing uncertainty for low sensitivity
rate parameters
A Clock Reaction Based on Molybdenum Blue
Clock reactions are rare kinetic
phenomena, so far limited mostly
to systems with ionic oxoacids and oxoanions in water. We report a
new clock reaction in cyclohexanol that forms molybdenum blue from
a noncharged, yellow molybdenum complex as precursor, in the presence
of hydrogen peroxide. Interestingly, the concomitant color change
is reversible, enabling multiple clock cycles to be executed consecutively.
The kinetics of the clock reaction were experimentally characterized,
and by adding insights from quantum chemical calculations, a plausible
reaction mechanism was postulated. Key elementary reaction steps comprise
sigmatropic rearrangements with five-membered or bicyclo[3.1.0] transition
states. Importantly, numerical kinetic modeling demonstrated the mechanism’s
ability to reproduce the experimental findings. It also revealed that
clock behavior is intimately connected to the sudden exhaustion of
hydrogen peroxide. Due to the stoichiometric coproduction of ketone,
the reaction bears potential for application in alcohol oxidation
catalysis
Facile Soft-Templated Synthesis of High-Surface Area and Highly Porous Carbon Nitrides
Mesoporous
carbon nitride is synthesized in a one-pot approach
using different nonionic surfactants (Pluronic F-127, Pluronic P-123,
and Triton X-100) and a melamine cyanurate hydrogen-bonded complex
using just water as the solvent. We obtain three-dimensional assembled
nanostructures from low-dimensional carbon nitride sheets by taking
advantage of supramolecular assembly of melamine and cyanuric acid,
moderate interactions between the surfactant and precursors, structure
directing effects of the surfactants, and the good thermal stability
of the melamine cyanurate sheets formed around the micelles. Different
morphologies, including sheetlike, hollow spherical, and tubular or
highly porous networks, result depending upon the synthesis approach
and the surfactant/precursor ratio. Pseudoternary phase diagrams map
the composition of the starting solution to the resultant carbon nitride
morphology. Increasing the amount of surfactant leads to a higher
carbon residue (C/N ∼ 1) and large BET surface areas (≤300
m<sup>2</sup>/g). Further tuning of the synthesis parameters as well
as addition of HCl produces uniformly porous nanostructures with a
high porosity (up to 0.8 cm<sup>3</sup>/g), a high surface area (>200
m<sup>2</sup>/g), and yet a stoichiometric C/N ratio (∼0.75).
The synthesized high-surface area carbon nitrides show improved light
absorption and enhanced photocatalytic activity in a rhodamine B dye
degradation reaction under visible light irradiation compared to those
of bulk melamine-derived carbon nitride
Characterization and Modeling of the Operating Curves of Membrane Microseparators
The
membrane microseparator is a milliliter-scale flow chemistry
module that continuously separates a biphasic flow through a PTFE
microporous membrane. It has found a wide range of applications in
the continuous manufacturing of active pharmaceutical ingredients
and fine chemicals, especially those involving multiple synthetic
steps. Yet, the accurate prediction and control of the pressure balance
needed for successful phase separations is technically challenging.
In this article, we present systematic modeling of the operating ranges
of the membrane microseparator. We characterize the retention and
breakthrough phenomena of the device and develop two new analytic
models for retention and breakthrough by taking into consideration
the tortuosity factor and pore size distribution. The new models are
shown to be better predictors of the experimental results than the
original theoretical models based on the simple Young–Laplace
equation and the straight-channel Hagen–Poiseuille equation
Multiphase Oscillatory Flow Strategy for <i>in Situ</i> Measurement and Screening of Partition Coefficients
Taking advantage of the difference
between the surface energies
of aqueous and organic solvents on a Teflon substrate, a fully automated
small-scale strategy is developed on the basis of gas-driven oscillatory
motion of a biphasic slug for high-throughput <i>in situ</i> measurement and screening of partition coefficients of organic substances
between aqueous and organic phases. The developed oscillatory flow
strategy enables single partition coefficient data point measurement
within 8 min (including the sample preparation time) which is 360
times faster than the conventional “shake-flask” method,
while using less than a 30 μL volume of the two phases and 9
nmol of the target organic substance. The developed multiphase strategy
is validated using a conventional shake-flask technique. Finally,
the developed strategy is extended to include automated screening
of partition coefficients at physiological temperature
High Throughput Synthesis of Uniform Biocompatible Polymer Beads with High Quantum Dot Loading Using Microfluidic Jet-Mode Breakup
Uniform polymer microbeads with highly
loaded quantum dots (QDs)
are produced using high-throughput coherent jet breakup of a biocompatible
poly(ethylene glycol) diacrylate (PEGDA) prepolymer resin, followed
by in-line photopolymerization. A spiraling and gradually widening
channel enables maximum absorption of radiated UV light for the in-line
photopolymerization without coalescence and clogging issues. Although
the dripping mode in general provides superior uniformity to the jet
mode, our nozzle design with tapered geometry brings controlled jet
breakup leading to 3% of uniform particle size distribution, comparable
to dripping-mode performance. We achieve a maximum production rate
of 2.32 kHz, 38 times faster than the dripping mode, at a same polymer
flow rate. In addition, the jet-mode scheme provides better versatility
with 3 times wider range of size control as well as the compatibility
with viscous fluids that could cause pressure buildup in the microsystem.
As a demonstration, a QD-doped prepolymer resin is introduced to create
uniform biocompatible polymer beads with 10 wt % CdSe/ZnSe QD loading.
In spite of this high loading, the resulting polymer beads exhibits
narrow bandwidth of 28 nm to be used for the ultrasensitive bioimaging,
optical coding, and sensing sufficiently with single bead