Heat-map with all selected VOCs from the five different MAP strains as well as from the media a) quantitative data b) normalized data; Cultures: 1=DSM 44133, 2=JIII-386, 3-5=JII-2421, JII-3197, JII-0817.

<p>Heat-map with all selected VOCs from the five different MAP strains as well as from the media a) quantitative data b) normalized data; Cultures: 1=DSM 44133, 2=JIII-386, 3-5=JII-2421, JII-3197, JII-0817.</p

Effect of density of bacterial growth on emitted amounts of VOCs.

<div><p><b>1: DSM 44133 (laboratory reference strain), 2: JIII-386 (sheep strain), 3-5: JII-2421, JII-3197, JII-0817 (field strains)</b>. </p> <p>a) 2-ethylfuran b) 2-methylfuran c) 2-pentylfuran d) ethyl acetate e) dimethyldisulfide f) 1-methyl-1-H-pyrrole.</p></div

Principal component analysis on VOCs emitted from 5 different MAP strains and the blank media. 1: DSM 44133 (laboratory reference strain), 2: JIII-386 (sheep strain), 3-5: JII-2421, JII-3197, JII-0817 (field strains).

<p>Principal component analysis on VOCs emitted from 5 different MAP strains and the blank media. 1: DSM 44133 (laboratory reference strain), 2: JIII-386 (sheep strain), 3-5: JII-2421, JII-3197, JII-0817 (field strains).</p

Chromatograms of selected ion traces (m/z 43 & m/z 81) from the headspace of the reference strain DSM 44133 (dilution of bacteria of 10^-2; red chromatograms) and blank media (blue chromatograms). Retention times of marker substances are described in table 2.

<p>Chromatograms of selected ion traces (m/z 43 & m/z 81) from the headspace of the reference strain DSM 44133 (dilution of bacteria of 10^-2; red chromatograms) and blank media (blue chromatograms). Retention times of marker substances are described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0076868#pone-0076868-t002" target="_blank">table 2</a>.</p

Experimental set up for alveolar breath sampling in goats.

<p>A—sampling mask; B—CO₂-sensor; C—Needle Trap Device (NTD); D—CO₂ triggered flow valve; E—Capnogard for time resolved CO₂-monitoring; F—sampling box; dashed arrows represent air flows; continuous arrows represent electronic signals.</p

Retention times, regression coefficients, detection limits and concentration ranges of selected VOC marker substances from breath (a) and feces (b).

<p>R²—coefficient of determination;LOD—limit of detection;LOQ—limit of quantification</p><p>* potential marker substances from a former in vitro study [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123980#pone.0123980.ref009" target="_blank">9</a>]</p><p>^† values lower than LOD</p><p>Retention times, regression coefficients, detection limits and concentration ranges of selected VOC marker substances from breath (a) and feces (b).</p

Mann-Whitney-U-tests for analyses from headspace over feces.

<p>*—p-value from t-test</p><p>Mann-Whitney-U-tests for analyses from headspace over feces.</p

Concentration of 1-propanol in breath gas- and room air-samples.

<p>Concentration of 1-propanol in breath gas- and room air-samples.</p

PCA-scatterplots based on VOC-analysis of breath.

<p>PCA (a) was done for all substances (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123980#pone.0123980.t002" target="_blank">Table 2</a>) having different concentrations in inoculated and non-inoculated animals. The 3D-scatterplot (b) is derived from the same PCA with respect to weeks after inoculation on the third axes. The loading plot referring to this PCA analysis is given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123980#pone.0123980.s004" target="_blank">S4 Fig</a>. Blue dots represent the non-inoculated group. Red dots represent the inoculated group. PC-01 explains 31% and PC-03 explains 15% of variance.</p

VOC-concentration ranges and differences in relation to MAP-specific antibody levels and interferon-γ response after stimulation with JPPD.

<p>p-values from Mann-Whitney-U-tests</p><p>^† value lower than limit of detection</p><p>* p-values from t-tests</p><p>VOC-concentration ranges and differences in relation to MAP-specific antibody levels and interferon-γ response after stimulation with JPPD.</p