ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation

Two side-by-side papers report that the transcription elongation factor ELOF1 drives transcription-coupled repair and prevents replication stress.Cells employ transcription-coupled repair (TCR) to eliminate transcription-blocking DNA lesions. DNA damage-induced binding of the TCR-specific repair factor CSB to RNA polymerase II (RNAPII) triggers RNAPII ubiquitylation of a single lysine (K1268) by the CRL4(CSA) ubiquitin ligase. How CRL4(CSA) is specifically directed towards K1268 is unknown. Here, we identify ELOF1 as the missing link that facilitates RNAPII ubiquitylation, a key signal for the assembly of downstream repair factors. This function requires its constitutive interaction with RNAPII close to K1268, revealing ELOF1 as a specificity factor that binds and positions CRL4(CSA) for optimal RNAPII ubiquitylation. Drug-genetic interaction screening also revealed a CSB-independent pathway in which ELOF1 prevents R-loops in active genes and protects cells against DNA replication stress. Our study offers key insights into the molecular mechanisms of TCR and provides a genetic framework of the interplay between transcriptional stress responses and DNA replication.Cancer Signaling networks and Molecular Therapeutic

Figuring out what they feel: exposure to eudaimonic narrative fiction is related to mentalizing ability

Being exposed to narrative fiction may provide us with practice in dealing with social interactions and thereby enhance our ability to engage in mentalizing (understanding other people’s mental states). The current study uses a confirmatory Bayesian approach to assess the relationship between mentalizing and both the self-reported frequency of exposure to narrative fiction across media (books, films, and TV series) and the particular types of fiction that are consumed (eudaimonic vs. hedonic). This study focuses on this relationship in children and adolescents, because they are still developing their social abilities. Exposure to narrative fiction may thus be particularly important in providing input on how to interpret other people’s mental states for this age group. In our study, we find no evidence for a simple relationship between overall frequency of narrative fiction exposure and mentalizing ability in this age group. However, exposure to eudaimonic narrative fiction is consistently positively related to mentalizing and, for some media types and aspects of mentalizing, more strongly so than exposure to hedonic narrative fiction. No evidence was obtained to suggest that there are any differential effects related to the medium of the narrative fiction exposure (written vs. visual).Theoretical and Experimental Linguistic