Aquaporins in the Colon as a New Therapeutic Target in Diarrhea and Constipation

Aquaporins (AQPs) play important roles in the water transport system in the human body. There are currently 13 types of AQP, AQP0 through AQP12, which are expressed in various organs. Many members of the AQP family are expressed in the intestinal tract. AQP3 is predominantly expressed in the colon, ultimately controlling the water transport. Recently, it was clarified that several laxatives exhibit a laxative effect by changing the AQP3 expression level in the colon. In addition, it was revealed that morphine causes severe constipation by increasing the AQP3 expression level in the colon. These findings have shown that AQP3 is one of the most important functional molecules in water transport in the colon. This review will focus on the physiological and pathological roles of AQP3 in the colon, and discuss clinical applications of colon AQP3

Primary Gastric T Cell Lymphoma with Epstein-Barr Virus Infection.

Primary gastric T cell malignant lymphomas are very rare. Here we report a case of primary gastric T cell lymphoma infected with Epstein-Barr virus (EBV). Histologic and immunohistologic features, positive for CD45RO/UCHL1 and negative for CD20/L26, showed this tumor to be a diffuse, large cell type, malignant lymphoma with a T cell phenotype. Genotypic analysis by the South-ern blot method, demonstrating T cell receptor gene rearrangement and a germ-line configuration of the immunoglobulin gene, also supported the T cell origin of this tumor. EBV DNA was detected by polymerase chain reaction and in situ hybridization against EBER1 demonstrated that EBV was located in the lymphoma cells. This is the first Japanese case report of primary gastric malig-nant lymphoma of T cell origin infected with EBV

Antibodies against nephronectin ameliorate anti‐type II collagen‐induced arthritis in mice

The extracellular matrix protein nephronectin (Npnt) is known to be critical for kidney development, but its function in inflammatory diseases is unknown. Here, we developed a new enzyme‐linked immunosorbent assay system to detect Npnt in various autoimmune diseases, which revealed that plasma Npnt levels are increased in various mouse autoimmune models. We also report that antibodies against the α8β1 integrin‐binding region of Npnt protect mice from anti‐type II collagen‐induced arthritis, suggesting that Npnt may be a potential therapeutic target molecule for the prevention of autoimmune arthritis

Relationship between Aging-Related Skin Dryness and Aquaporins

Skin function deteriorates with aging, and the dermal water content decreases. In this study, we have analyzed the mechanism of aging-related skin dryness focusing on aquaporins (AQPs), which are the water channels. Mice aged 3 and 20 months were designated as young and aged mice, respectively, to be used in the experiments. No differences were observed in transepidermal water loss between the young mice and aged mice. However, the dermal water content in aged mice was significantly lower than that in young mice, thus showing skin dryness. The expression of AQP1, AQP3, AQP4, AQP7, and AQP9 was observed in the skin. All the mRNA expression levels of these AQPs were significantly lower in aged mice. For AQP3, which was expressed dominantly in the skin, the protein level was lower in aged mice than in young mice. The results of the study showed that the expression level of AQPs in the skin decreased with aging, suggesting the possibility that this was one of the causes of skin dryness. New targets for the prevention and treatment of aging-related skin dryness are expected to be proposed when the substance that increases the expression of AQP3 is found