Rapid prototyping of electrochemical lateral flow devices: stencilled electrodes

A straightforward and very cost effective method is proposed to prototype electrodes using pressure sensitive adhesives (PSA) and a simple cutting technique. Two cutting methods, namely blade cutting and CO2 laser ablation, are compared and their respective merits are discussed. The proposed method consists of turning the protective liner on the adhesive into a stencil to apply screen-printing pastes. After the electrodes have been printed, the liner is removed and the PSA can be used as a backing material for standard lateral flow membranes. We present the fabrication of band electrodes down to 250 μm wide, and their characterization using microscopy techniques and cyclic voltammetry. The prototyping approach presented here facilitates the development of new electrochemical devices even if very limited fabrication resources are available. Here we demonstrate the fabrication of a simple lateral-flow device capable of determining glucose in blood. The prototyping approach presented here is highly suitable for the development of novel electroanalytical tools.This work has been funded by the Spanish Ministry of Economy through the DADDi2 project (Grant TEC2013-48506). MK acknowledges funding through the Beatriu de Pinós program (BP-DGR-2013), supported by the Secretary for Universities and Research of the Ministry of Economy and Knowledge of the Government of Catalonia and the Cofund programme of the Marie Curie Actions of the 7th R&D Framework Programme of the European Union. We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI)Peer reviewe

Electrochromic biosensor

[ES] Biosensor electrocrómico. La presente invención se refiere a un biosensor formado por un ánodo de un material conductor que contiene al menos una enzima inmovilizada sobre su superficie y un mediador redox y un cátodo de un material transparente que contiene una molécula electrocrómica que cambia de color cuando cambia su estado de oxidación. Entre ánodo y cátodo se deposita un medio conductor de iones que puede estar en forma de gel o de membrana permeable. Este biosensor se activa automáticamente cuando se deposita una muestra que contenga el analito cuya oxidación/reducción enzimática genera un flujo de electrones desde el ánodo al cátodo que provoca el cambio de color del material electrocrómico y que da información inmediata al usuario de la presencia de dicho analito en la muestra.[EN] The invention relates to a biosensor formed by an anode of a conductive material which contains at least one enzyme immobilised on the surface thereof and a redox mediator, and a cathode of a transparent material which contains an electrochromic molecule that changes colour when its state of oxidation changes. An ion-conductive medium in the form of a permeable membrane or gel is deposited between the anode and the cathode. This biosensor is activated automatically by the deposition of a sample containing an analyte generating an electron flow from the anode to the cathode by the enzymatic reduction/oxidation thereof, which causes the change in colour of the electrochromic material and provides immediate information to the user about the presence of said analyte in the sample.Peer reviewedConsejo Superior de Investigaciones Científicas (España)A1 Solicitud de patente con informe sobre el estado de la técnic

Electrochromic biosensor

[ES] Biosensor electrocrómico. La presente invención se refiere a un biosensor formado por un ánodo de un material conductor que contiene al menos una enzima inmovilizada sobre su superficie y un mediador redox y un cátodo de un material transparente que contiene una molécula electrocrómica que cambia de color cuando cambia su estado de oxidación. Entre ánodo y cátodo se deposita un medio conductor de iones que puede estar en forma de gel o de membrana permeable. Este biosensor se activa automáticamente cuando se deposita una muestra que contenga el analito cuya oxidación/reducción enzimática genera un flujo de electrones desde el ánodo al cátodo que provoca el cambio de color del material electrocrómico y que da información inmediata al usuario de la presencia de dicho analito en la muestra.[EN] The invention relates to a biosensor formed by an anode of a conductive material which contains at least one enzyme immobilised on the surface thereof and a redox mediator, and a cathode of a transparent material which contains an electrochromic molecule that changes colour when its state of oxidation changes. An ion-conductive medium in the form of a permeable membrane or gel is deposited between the anode and the cathode. This biosensor is activated automatically by the deposition of a sample containing an analyte generating an electron flow from the anode to the cathode by the enzymatic reduction/oxidation thereof, which causes the change in colour of the electrochromic material and provides immediate information to the user about the presence of said analyte in the sample.Peer reviewedConsejo Superior de Investigaciones Científicas (España)B1 Patente sin examen previ

Biosensor electrocrómico

[EN] The invention relates to a biosensor formed by an anode of a conductive material which contains at least one enzyme immobilised on the surface thereof and a redox mediator, and a cathode of a transparent material which contains an electrochromic molecule that changes colour when its state of oxidation changes. An ion-conductive medium in the form of a permeable membrane or gel is deposited between the anode and the cathode. This biosensor is activated automatically by the deposition of a sample containing an analyte generating an electron flow from the anode to the cathode by the enzymatic reduction/oxidation thereof, which causes the change in colour of the electrochromic material and provides immediate information to the user about the presence of said analyte in the sample.[ES] Biosensor electrocrómico. La presente invención se refiere a un biosensor formado por un ánodo de un material conductor que contiene al menos una enzima inmovilizada sobre su superficie y un mediador redox y un cátodo de un material transparente que contiene una molécula electrocrómica que cambia de color cuando cambia su estado de oxidación. Entre ánodo y cátodo se deposita un medio conductor de iones que puede estar en forma de gel o de membrana permeable. Este biosensor se activa automáticamente cuando se deposita una muestra que contenga el analito cuya oxidación/reducción enzimática genera un flujo de electrones desde el ánodo al cátodo que provoca el cambio de color del material electrocrómico y que da información inmediata al usuario de la presencia de dicho analito en la muestra.Peer reviewedConsejo Superior de Investigaciones Científicas (España)A1 Solicitud de patente con informe sobre el estado de la técnic