Evaluation of the Effects of Honey on Acute-Phase Deep Burn Wounds

This study aimed to clarify the effects of honey on acute-phase deep burn wounds. Two deep burn wounds were created on mice which were divided into four groups: no treatment, silver sulfadiazine, manuka honey, and Japanese acacia honey. Wound sizes were calculated as expanded wound areas and sampled 30 minutes and 1–4 days after wounding for histological observation. The wound sections were subjected to hematoxylin and eosin and immunohistological staining to detect necrotic cells, apoptotic cells, neutrophils, and macrophages. The no treatment group formed a scar. The redness around the wound edges in the silver sulfadiazine group was the most intense. All groups exhibited increased wound areas after wounding. The proportions of necrotic cells and the numbers of neutrophils in the manuka and acacia honey groups were lower than those in the no treatment and silver sulfadiazine groups until day 3; however, there were no significant differences between all groups on day 4. These results show that honey treatment on deep burn wounds cannot prevent wound progression. Moreover, comparing our observations with those of Jackson, there are some differences between humans and animals in this regard, and the zone of hyperemia and its surrounding area fall into necrosis, which contributes to burn wound progression

Evaluation of the Effects of Honey on Acute-Phase Deep Burn Wounds

This study aimed to clarify the effects of honey on acute-phase deep burn wounds. Two deep burn wounds were created on mice which were divided into four groups: no treatment, silver sulfadiazine, manuka honey, and Japanese acacia honey. Wound sizes were calculated as expanded wound areas and sampled 30 minutes and 1–4 days after wounding for histological observation. The wound sections were subjected to hematoxylin and eosin and immunohistological staining to detect necrotic cells, apoptotic cells, neutrophils, and macrophages. The no treatment group formed a scar. The redness around the wound edges in the silver sulfadiazine group was the most intense. All groups exhibited increased wound areas after wounding. The proportions of necrotic cells and the numbers of neutrophils in the manuka and acacia honey groups were lower than those in the no treatment and silver sulfadiazine groups until day 3; however, there were no significant differences between all groups on day 4. These results show that honey treatment on deep burn wounds cannot prevent wound progression. Moreover, comparing our observations with those of Jackson, there are some differences between humans and animals in this regard, and the zone of hyperemia and its surrounding area fall into necrosis, which contributes to burn wound progression

Effects of Three Types of Japanese Honey on Full-Thickness Wound in Mice

Although many previous studies reported that honey promotes wound healing, no study has examined the effects of Japanese honey. The aim of this study was to investigate the effects of three types of Japanese honey, Acacia, Buckwheat flour, and Chinese milk vetch honey, on wound healing in comparison with hydrocolloid dressing. Circular full-thickness skin wounds were produced on male mice. Japanese honey or hydrocolloid dressing was applied daily to the mice for 14 days. The ratio of wound area for the hydrocolloid dressing group increased initially in the inflammatory and early proliferative phases and then decreased rapidly to heal with scarring. However, the ratios of wound area for the Japanese honey groups decreased in the inflammatory phase, increased in the proliferative phase, and decreased in the proliferative phase, and some wounds were not completely covered with new epithelium. These findings indicate that using Japanese honey alone has limited benefit, but since it reduces wound size in the inflammatory phase, it is possible to apply a combined treatment in which Japanese honey is applied only in the inflammatory phase, followed by hydrocolloid dressing from the proliferative phase, which would effectively contract the wound

マウス皮膚における創周囲皮膚の清潔が創傷治癒過程に及ぼす影響

金沢大学医薬保健研究域保健学系［背景］褥瘡治癒には局所環境の整えが重要であり, 創洗浄だけでなく創周囲皮膚の清潔ケアも提唱されている. 目的：創周囲皮膚に対しどのような清潔ケアが治癒過程を促進するか, マウスを用いて検討した. ［方法］マウスに全層創傷を作製し創周囲を弱酸性の液体石鹸(以下「皮膚洗浄剤」)で洗浄, 生理食塩水(以下「生食」)洗浄, ポビドンヨード消毒, ケアなしの4群に分けてケア後, 毎日創周囲に黄色ブドウ球菌を塗付し, 創傷被覆材で覆った. 肉眼的所見, 治癒期間, 細菌叢, 創周囲角質水分量を測定し, 組織所見の変化と合わせて4群を比較した. ［結果］表皮化の速度, 肉眼的変化, 治癒時の創周囲角質水分量, 平均治癒期間, 組織学的皮膚構造変化の点で, 皮膚洗浄剤群が最も治癒促進していた. ついでポビドンヨード群, 生食群, ケアなし群の順であった. ［結語］皮膚洗浄剤による創周囲皮膚の清潔が, 創傷治癒を促進させる最も有効な局所ケア方法であることが示唆された.For wound healing, managing the wound and its environs is crucial. It is unclear, however, whether cleansing the skin surrounding wounds promotes wound healing. Thus, the aim of this study is to examine wound healing under four different care regimens of full- thickness skin wounds on mice：1. cleansing the skin surrounding wounds with detergent, 2. saline, 3. povidone-iodine, or 4. no care. Four full-thickness skin wounds were made on backs of mice. The above-described care regimens were performed, then Staphylococcus aureus was applied to the skin surrounding the wounds, and the wounds and the surrounding skin were covered with hydrocolloid dressings. These interventions were performed every day. The period of wound healing was 8 + 0.8 days in the detergent group, 8.8 + 1.5 days in the saline group, 9 + 0.8 days in the povidone-iodine group, and 10.3 + 0.96 days in the no care group. There was a significant difference between the detergent and the no care groups. In histological findings, reepithelization of the detergent group was achieved 7 days after wounding, and fibrous granulation tissue of the no care group on day 14 was the slowest to develop. These findings indicate that to cleanse the skin surrounding wounds with detergent is the most effective care in promoting wound healing, and detergent care can be effective for the care of pressure ulcers contaminated with bacteria.日本褥瘡学会の許可を得て登録_2021.9.2